Journal: Frontiers in Microbiology

Article Title: Evaluation of physical and chemical isolation methods to extract and purify Campylobacter jejuni extracellular polymeric substances

doi: 10.3389/fmicb.2024.1488114

Figure Lengend Snippet: Analysis results for determining the presence of major extracellular matrix components in samples prepared using different methods for isolating the extracellular matrix of C. jejuni , including the estimation of total isolation time and assessment of isolation performance.

Article Snippet: The Dowex cation exchanger was prepared by adding 1 g of Dowex cation exchange resin (Supelco 44514, Merck, Germany) to 10 ml of extraction buffer, mixing well with an automatic pipette, and incubating for 15 min.

Techniques: Isolation, Agarose Gel Electrophoresis, Protein Concentration, Staining, Concentration Assay, Centrifugation

Journal: Frontiers in Microbiology

Article Title: Evaluation of physical and chemical isolation methods to extract and purify Campylobacter jejuni extracellular polymeric substances

doi: 10.3389/fmicb.2024.1488114

Figure Lengend Snippet: Polysaccharides in the ECM isolates of Campylobacter jejuni . (A) Polysaccharide content as revealed by the phenol-sulphuric acid method. The mean values and standard deviations of two measurements are shown. Dunnett’s multiple comparisons with the control cell lysate tested at significance levels of * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001. The ANOVA results were significant [ F (9, 3) = 60.98, p < 0.0001]. (B) SDS-PAGE analysis with periodic acid-Schiff staining. (C) SDS-PAGE analysis with Alcian blue staining. Samples were obtained by sodium chloride isolation (A), centrifugation (B), a procedure that yielded a supernatant of weakly bound extracellular matrix components (SV), heating in sodium carbonate (C), EDTA (D), sodium hydroxide (E), formaldehyde and sodium hydroxide (F), Dowex cation exchanger (G) and ether solution (H). Total cell lysate (CL) was also tested.

Article Snippet: The Dowex cation exchanger was prepared by adding 1 g of Dowex cation exchange resin (Supelco 44514, Merck, Germany) to 10 ml of extraction buffer, mixing well with an automatic pipette, and incubating for 15 min.

Techniques: Control, SDS Page, Staining, Isolation, Centrifugation

Journal: Frontiers in Microbiology

Article Title: Evaluation of physical and chemical isolation methods to extract and purify Campylobacter jejuni extracellular polymeric substances

doi: 10.3389/fmicb.2024.1488114

Figure Lengend Snippet: Proteins in the ECM isolates of Campylobacter jejuni . (A) Protein content as revealed by the DC Protein Assay. The mean values and standard deviations of three measurements are shown. Dunnett’s multiple comparisons with the control cell lysate tested at significance levels of p ** < 0.01 and **** p < 0.0001. The ANOVA results were significant [ F (7, 16) = 286.1, p < 0.0001]. (B) SDS-PAGE analysis with Coomassie Brilliant Blue staining. Samples were obtained by sodium chloride isolation (A), centrifugation (B), a procedure that yielded a supernatant of weakly bound extracellular matrix components (SV), heating in sodium carbonate (C), EDTA (D), sodium hydroxide (E), formaldehyde and sodium hydroxide (F), Dowex cation exchanger (G) and ether solution (H). Total cell lysate (CL) was also tested.

Article Snippet: The Dowex cation exchanger was prepared by adding 1 g of Dowex cation exchange resin (Supelco 44514, Merck, Germany) to 10 ml of extraction buffer, mixing well with an automatic pipette, and incubating for 15 min.

Techniques: DC Protein Assay, Control, SDS Page, Staining, Isolation, Centrifugation

Journal: Frontiers in Microbiology

Article Title: Evaluation of physical and chemical isolation methods to extract and purify Campylobacter jejuni extracellular polymeric substances

doi: 10.3389/fmicb.2024.1488114

Figure Lengend Snippet: Extracellular DNA in the ECM isolates of Campylobacter jejuni . A representative example of agarose gel electrophoresis. Samples were obtained by sodium chloride isolation (A), centrifugation (B), a procedure that yielded a supernatant of weakly bound extracellular matrix components (SV), heating in sodium carbonate (C), EDTA (D), sodium hydroxide (E), formaldehyde and sodium hydroxide (F), Dowex cation exchanger (G) and ether solution (H). Total cell lysate (CL) was also tested.

Article Snippet: The Dowex cation exchanger was prepared by adding 1 g of Dowex cation exchange resin (Supelco 44514, Merck, Germany) to 10 ml of extraction buffer, mixing well with an automatic pipette, and incubating for 15 min.

Techniques: Agarose Gel Electrophoresis, Isolation, Centrifugation