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human atp7b sirna  (Santa Cruz Biotechnology)
90
Santa Cruz Biotechnology human atp7b sirna
Hepatic functions and <t>ATP7B</t> expression of SHED-Heps. ( a – e ) In vitro hepatic function assays of SHED-Heps. Culture of SHED-Heps and SHED and measuring of human albumin (hALB), glucose, triglyceride (TG), and urea in the conditioned medium are performed according to the Methods. ( a ) Xenobiotic activity of SHED-Heps and SHED via CYP3A4 is analyzed under dexamethasone stimulation (50 μM). ( b ) Low density lipoprotein (LDL) uptake and bile acid transport are analyzed by DiI-Ac-LDL ( c ) and cholyl-lysyl-fluorescein (CLF) ( d ) staining, respectively. ( e – g ) QRT-PCR shows the expression of <t>ATPase</t> <t>copper</t> <t>transporting</t> beta gene ( ATP7B ) in SHED and SHED-Heps. The results are shown as the ratio to the expression in SHED- Heps ( e ). Immunofluorescent staining demonstrates the expression of ATP7B in SHED and SHED- Heps. Control IgG, isotype-matched IgG staining. ( f ) QRT-PCR shows the effect of ATP7B siRNA treatment in SHED and SHED-Heps. The results are shown as the ratio to the expression of 18S ribosomal RNA ( 18S ). siATP7B, ATP7B siRNA pre-treatment; siCTRL, scrambled control siRNA pre-treatment ( g ) Immunofluorescent staining shows the effect of ATP7B. siRNA treatment in SHED and SHED-Heps. ( h ) ( a , b , e , g ) n = 3 for all groups. **P < 0.01, ***P < 0.005. Graph bars show the means ± SEM. ( c , d , f , h ) Nuclei are stained with DAPI. Bars = 30 μm ( c , d ), 20 μm ( f , h ).
Human Atp7b Sirna, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human atp7b sirna/product/Santa Cruz Biotechnology
Average 90 stars, based on 1 article reviews
human atp7b sirna - by Bioz Stars, 2026-02
90/100 stars
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control short hairpin sh rna plasmid  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology control short hairpin sh rna plasmid
Hepatic functions and <t>ATP7B</t> expression of SHED-Heps. ( a – e ) In vitro hepatic function assays of SHED-Heps. Culture of SHED-Heps and SHED and measuring of human albumin (hALB), glucose, triglyceride (TG), and urea in the conditioned medium are performed according to the Methods. ( a ) Xenobiotic activity of SHED-Heps and SHED via CYP3A4 is analyzed under dexamethasone stimulation (50 μM). ( b ) Low density lipoprotein (LDL) uptake and bile acid transport are analyzed by DiI-Ac-LDL ( c ) and cholyl-lysyl-fluorescein (CLF) ( d ) staining, respectively. ( e – g ) QRT-PCR shows the expression of <t>ATPase</t> <t>copper</t> <t>transporting</t> beta gene ( ATP7B ) in SHED and SHED-Heps. The results are shown as the ratio to the expression in SHED- Heps ( e ). Immunofluorescent staining demonstrates the expression of ATP7B in SHED and SHED- Heps. Control IgG, isotype-matched IgG staining. ( f ) QRT-PCR shows the effect of ATP7B siRNA treatment in SHED and SHED-Heps. The results are shown as the ratio to the expression of 18S ribosomal RNA ( 18S ). siATP7B, ATP7B siRNA pre-treatment; siCTRL, scrambled control siRNA pre-treatment ( g ) Immunofluorescent staining shows the effect of ATP7B. siRNA treatment in SHED and SHED-Heps. ( h ) ( a , b , e , g ) n = 3 for all groups. **P < 0.01, ***P < 0.005. Graph bars show the means ± SEM. ( c , d , f , h ) Nuclei are stained with DAPI. Bars = 30 μm ( c , d ), 20 μm ( f , h ).
Control Short Hairpin Sh Rna Plasmid, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/control short hairpin sh rna plasmid/product/Santa Cruz Biotechnology
Average 93 stars, based on 1 article reviews
control short hairpin sh rna plasmid - by Bioz Stars, 2026-02
93/100 stars
  Buy from Supplier

Image Search Results


Hepatic functions and ATP7B expression of SHED-Heps. ( a – e ) In vitro hepatic function assays of SHED-Heps. Culture of SHED-Heps and SHED and measuring of human albumin (hALB), glucose, triglyceride (TG), and urea in the conditioned medium are performed according to the Methods. ( a ) Xenobiotic activity of SHED-Heps and SHED via CYP3A4 is analyzed under dexamethasone stimulation (50 μM). ( b ) Low density lipoprotein (LDL) uptake and bile acid transport are analyzed by DiI-Ac-LDL ( c ) and cholyl-lysyl-fluorescein (CLF) ( d ) staining, respectively. ( e – g ) QRT-PCR shows the expression of ATPase copper transporting beta gene ( ATP7B ) in SHED and SHED-Heps. The results are shown as the ratio to the expression in SHED- Heps ( e ). Immunofluorescent staining demonstrates the expression of ATP7B in SHED and SHED- Heps. Control IgG, isotype-matched IgG staining. ( f ) QRT-PCR shows the effect of ATP7B siRNA treatment in SHED and SHED-Heps. The results are shown as the ratio to the expression of 18S ribosomal RNA ( 18S ). siATP7B, ATP7B siRNA pre-treatment; siCTRL, scrambled control siRNA pre-treatment ( g ) Immunofluorescent staining shows the effect of ATP7B. siRNA treatment in SHED and SHED-Heps. ( h ) ( a , b , e , g ) n = 3 for all groups. **P < 0.01, ***P < 0.005. Graph bars show the means ± SEM. ( c , d , f , h ) Nuclei are stained with DAPI. Bars = 30 μm ( c , d ), 20 μm ( f , h ).

Journal: Scientific Reports

Article Title: Therapeutic potential of hepatocyte-like-cells converted from stem cells from human exfoliated deciduous teeth in fulminant Wilson’s disease

doi: 10.1038/s41598-018-38275-y

Figure Lengend Snippet: Hepatic functions and ATP7B expression of SHED-Heps. ( a – e ) In vitro hepatic function assays of SHED-Heps. Culture of SHED-Heps and SHED and measuring of human albumin (hALB), glucose, triglyceride (TG), and urea in the conditioned medium are performed according to the Methods. ( a ) Xenobiotic activity of SHED-Heps and SHED via CYP3A4 is analyzed under dexamethasone stimulation (50 μM). ( b ) Low density lipoprotein (LDL) uptake and bile acid transport are analyzed by DiI-Ac-LDL ( c ) and cholyl-lysyl-fluorescein (CLF) ( d ) staining, respectively. ( e – g ) QRT-PCR shows the expression of ATPase copper transporting beta gene ( ATP7B ) in SHED and SHED-Heps. The results are shown as the ratio to the expression in SHED- Heps ( e ). Immunofluorescent staining demonstrates the expression of ATP7B in SHED and SHED- Heps. Control IgG, isotype-matched IgG staining. ( f ) QRT-PCR shows the effect of ATP7B siRNA treatment in SHED and SHED-Heps. The results are shown as the ratio to the expression of 18S ribosomal RNA ( 18S ). siATP7B, ATP7B siRNA pre-treatment; siCTRL, scrambled control siRNA pre-treatment ( g ) Immunofluorescent staining shows the effect of ATP7B. siRNA treatment in SHED and SHED-Heps. ( h ) ( a , b , e , g ) n = 3 for all groups. **P < 0.01, ***P < 0.005. Graph bars show the means ± SEM. ( c , d , f , h ) Nuclei are stained with DAPI. Bars = 30 μm ( c , d ), 20 μm ( f , h ).

Article Snippet: SHED and SHED-Heps were treated for 48 h with Lipofectamine RNAiMAX (Thermo Fisher Scientific) mixed with human ATP7B siRNA (20 nM; Santa Cruz Biotechnology, Santa Cruz, CA), human stanniocalcin 1 (STC1) siRNA (20 nM; Santa Cruz Biotechnology, Santa Cruz), and the corresponding control scrambled siRNA (Santa Cruz Biotechnology).

Techniques: Expressing, In Vitro, Activity Assay, Staining, Quantitative RT-PCR, Control

Suppressive effects and integration of transplanted SHED-Heps on copper accumulated hepatic failure in fulminant LEC rats. SHED and SHED-Heps are transplanted in copper-overloaded LEC rats at 6 weeks of the age. ( a , b ) Histological assays of liver tissues at 10 weeks of the age. Representative images of liver tissues are analyzed by hematoxylin and eosin staining (HE). CV , central vein. ( a ) Copper accumulation is analyzed by Rhodanine staining. Yellow arrows, copper deposition. ( b , c ) Biochemical assay shows the copper contents in the liver tissues at 10 weeks of the age. ( d , e ) Post-transplant kinetics of DiR- labeled SHED and SHED-Hep cells in fulminant LEC rats after 2 weeks (2w) of SHED- and SHED- Hep-transplantation. In vivo tracing shows that DiR labeling is detected in the part of liver of rats. ( d ) Ex vivo tracing shows that DiR labeling is detected in liver and spleen, but not in lung and kidney, of rats. ( e , f , g ) Integration of transplanted SHED- and SHED-Heps in the liver tissues of fulminant LEC rats after 4 weeks of the transplantation. Immunohistochemial assay demonstrates the localization of human albumin (hALB) positive cells in the parenchyma of recipient liver tissues at 10 weeks of the age. Nuclei are stained with hematoxylin. ( f ) Double immunofluorescence shows that localization of human albumin (hALB, red) and human ATP7B (hATP7B, green) in the parenchymal cells of recipient liver tissues of SHED- and SHED-Hep-transplanted fulminant LEC rats. Nuclei are stained with DAPI. ( g ) ( a – g ) LEA, control LEA rats; LEC, non-transplanted fulminant LEC rats; SHED-T, SHED-transplanted fulminant LEC rats; SHED-Hep-T, SHED-Hep-transplanted fulminant LEC rats. ( a , b , f , g ) Bars = 50 μm ( a ), 100 μm ( b , f ), 30 μm ( g ). ( c ) n = 3 for all groups. Graph bars show the means ± SD. *P < 0.05 and ***P < 0.005.

Journal: Scientific Reports

Article Title: Therapeutic potential of hepatocyte-like-cells converted from stem cells from human exfoliated deciduous teeth in fulminant Wilson’s disease

doi: 10.1038/s41598-018-38275-y

Figure Lengend Snippet: Suppressive effects and integration of transplanted SHED-Heps on copper accumulated hepatic failure in fulminant LEC rats. SHED and SHED-Heps are transplanted in copper-overloaded LEC rats at 6 weeks of the age. ( a , b ) Histological assays of liver tissues at 10 weeks of the age. Representative images of liver tissues are analyzed by hematoxylin and eosin staining (HE). CV , central vein. ( a ) Copper accumulation is analyzed by Rhodanine staining. Yellow arrows, copper deposition. ( b , c ) Biochemical assay shows the copper contents in the liver tissues at 10 weeks of the age. ( d , e ) Post-transplant kinetics of DiR- labeled SHED and SHED-Hep cells in fulminant LEC rats after 2 weeks (2w) of SHED- and SHED- Hep-transplantation. In vivo tracing shows that DiR labeling is detected in the part of liver of rats. ( d ) Ex vivo tracing shows that DiR labeling is detected in liver and spleen, but not in lung and kidney, of rats. ( e , f , g ) Integration of transplanted SHED- and SHED-Heps in the liver tissues of fulminant LEC rats after 4 weeks of the transplantation. Immunohistochemial assay demonstrates the localization of human albumin (hALB) positive cells in the parenchyma of recipient liver tissues at 10 weeks of the age. Nuclei are stained with hematoxylin. ( f ) Double immunofluorescence shows that localization of human albumin (hALB, red) and human ATP7B (hATP7B, green) in the parenchymal cells of recipient liver tissues of SHED- and SHED-Hep-transplanted fulminant LEC rats. Nuclei are stained with DAPI. ( g ) ( a – g ) LEA, control LEA rats; LEC, non-transplanted fulminant LEC rats; SHED-T, SHED-transplanted fulminant LEC rats; SHED-Hep-T, SHED-Hep-transplanted fulminant LEC rats. ( a , b , f , g ) Bars = 50 μm ( a ), 100 μm ( b , f ), 30 μm ( g ). ( c ) n = 3 for all groups. Graph bars show the means ± SD. *P < 0.05 and ***P < 0.005.

Article Snippet: SHED and SHED-Heps were treated for 48 h with Lipofectamine RNAiMAX (Thermo Fisher Scientific) mixed with human ATP7B siRNA (20 nM; Santa Cruz Biotechnology, Santa Cruz, CA), human stanniocalcin 1 (STC1) siRNA (20 nM; Santa Cruz Biotechnology, Santa Cruz), and the corresponding control scrambled siRNA (Santa Cruz Biotechnology).

Techniques: Staining, Labeling, Transplantation Assay, In Vivo, Ex Vivo, Immunofluorescence, Control

In vitro copper metabolism of SHED-Heps. ( a – c ) In vitro copper metabolism assay in SHED-Heps and SHED via ATP7B. SHED, SHED-Heps, and ATP7B-knock-downed SHED-Heps are cultured for 6 h under CuSO 4 (600 μM) treatment and are subsequently cultured without CuSO 4 . Intracellular accumulated copper is measured at indicated time. Biochemical assay shows the intracellular copper contents in SHED and SHED-Heps ( a ), ATP7B- knock-downed SHED ( b ), and ATP7B-knock-downed SHED-Heps ( c ). ( d – f ) I n vitro survival assay in SHED and SHED-Heps via ATP7B under copper stimulation. SHED, SHED-Heps, and ATP7B-knock-downed SHED-Heps are cultured under the different stimulation of CuSO 4 (0, 125, 250, and 500 μM). The cell viability of the cells is measured after 3 days of the stimulation. Biochemical assay shows the viability of SHED and SHED-Heps ( d ), ATP7B-knock-downed SHED ( e ), and ATP7B- knock-downed SHED-Heps ( f ). ( g , h ) ATP7B-siRNA-treated SHED and ATP7B-siRNA-treated SHED-Heps are transplanted into fulminant LEC rats at 6 weeks of the age. The survival of LEC rats transplanted with ATP7B-siRNA-treated SHED (si ATP7B -SHED-T; ( g ), n = 5) and ATP7B-siRNA- treated SHED-Heps (si ATP7B -SHED-Hep-T; ( h ), n = 5) is assay by Kaplan-Meier curve. ( a – f) The results are shown as the ratio to the copper concentration at 0 h in each group. n = 3 for all groups. *P < 0.05 and ***P < 0.005. ns: no significance. Graph bars show the means ± SEM. ( a ) ††† P < 0.005 (vs. SHED at each tipe point). NS: no significance (vs. SHED at each tipe point). ( b , c , e , f ) siATP7B, ATP7B-siRNA treatment; siCTRL, control scrambled siRNA treatment. ( d ) ### P < 0.005 (vs. 0 h of each group). NS: no significance (vs. 0 h of each group). ( g , h ) CTRL-siRNA-SHED-Hep-T, fulminant LEC rats transplanted with control-siRNA-treated SHED (n = 5).

Journal: Scientific Reports

Article Title: Therapeutic potential of hepatocyte-like-cells converted from stem cells from human exfoliated deciduous teeth in fulminant Wilson’s disease

doi: 10.1038/s41598-018-38275-y

Figure Lengend Snippet: In vitro copper metabolism of SHED-Heps. ( a – c ) In vitro copper metabolism assay in SHED-Heps and SHED via ATP7B. SHED, SHED-Heps, and ATP7B-knock-downed SHED-Heps are cultured for 6 h under CuSO 4 (600 μM) treatment and are subsequently cultured without CuSO 4 . Intracellular accumulated copper is measured at indicated time. Biochemical assay shows the intracellular copper contents in SHED and SHED-Heps ( a ), ATP7B- knock-downed SHED ( b ), and ATP7B-knock-downed SHED-Heps ( c ). ( d – f ) I n vitro survival assay in SHED and SHED-Heps via ATP7B under copper stimulation. SHED, SHED-Heps, and ATP7B-knock-downed SHED-Heps are cultured under the different stimulation of CuSO 4 (0, 125, 250, and 500 μM). The cell viability of the cells is measured after 3 days of the stimulation. Biochemical assay shows the viability of SHED and SHED-Heps ( d ), ATP7B-knock-downed SHED ( e ), and ATP7B- knock-downed SHED-Heps ( f ). ( g , h ) ATP7B-siRNA-treated SHED and ATP7B-siRNA-treated SHED-Heps are transplanted into fulminant LEC rats at 6 weeks of the age. The survival of LEC rats transplanted with ATP7B-siRNA-treated SHED (si ATP7B -SHED-T; ( g ), n = 5) and ATP7B-siRNA- treated SHED-Heps (si ATP7B -SHED-Hep-T; ( h ), n = 5) is assay by Kaplan-Meier curve. ( a – f) The results are shown as the ratio to the copper concentration at 0 h in each group. n = 3 for all groups. *P < 0.05 and ***P < 0.005. ns: no significance. Graph bars show the means ± SEM. ( a ) ††† P < 0.005 (vs. SHED at each tipe point). NS: no significance (vs. SHED at each tipe point). ( b , c , e , f ) siATP7B, ATP7B-siRNA treatment; siCTRL, control scrambled siRNA treatment. ( d ) ### P < 0.005 (vs. 0 h of each group). NS: no significance (vs. 0 h of each group). ( g , h ) CTRL-siRNA-SHED-Hep-T, fulminant LEC rats transplanted with control-siRNA-treated SHED (n = 5).

Article Snippet: SHED and SHED-Heps were treated for 48 h with Lipofectamine RNAiMAX (Thermo Fisher Scientific) mixed with human ATP7B siRNA (20 nM; Santa Cruz Biotechnology, Santa Cruz, CA), human stanniocalcin 1 (STC1) siRNA (20 nM; Santa Cruz Biotechnology, Santa Cruz), and the corresponding control scrambled siRNA (Santa Cruz Biotechnology).

Techniques: In Vitro, Cell Culture, Clonogenic Cell Survival Assay, Concentration Assay, Control