Journal: Oncotarget

Article Title: miRNA-141 attenuates UV-induced oxidative stress via activating Keap1-Nrf2 signaling in human retinal pigment epithelium cells and retinal ganglion cells

doi: 10.18632/oncotarget.14489

Figure Lengend Snippet: Listed protein and mRNA expressions in stable RPEs (A) and (B) and RGCs (C) and (D) with miR-141 (two lines, “L1/L2”) or scramble miRNA control (“miR-C”) were examined by Western blot assay ( A and C ) and RT-qPCR assay ( B and D ), respectively. Nuclear Nrf2 expression was also shown ( A and C , lower panels). Protein expressions of Nrf2, HO1 and NQO1 ( vs . β-actin) were quantified ( A and C ). For each assay, n=5. Experiments in this figure were repeated four times to insure consistency of results. * p <0.05 vs . “Parental” cells ( A – D ).

Article Snippet: The two non-overlapping lentiviral Nrf2 shRNAs, Nrf2 shRNA-A (sc-37030-V) and Nrf2 shRNA-B (sc-44332-V), along with lentiviral scramble control shRNA, were purchased from Santa Cruz Biotech (Santa Cruz); The lentiviral shRNA (10 μL/mL) was added directly to cultured cells for 36 hours.

Techniques: Control, Western Blot, Quantitative RT-PCR, Expressing

Journal: Oncotarget

Article Title: miRNA-141 attenuates UV-induced oxidative stress via activating Keap1-Nrf2 signaling in human retinal pigment epithelium cells and retinal ganglion cells

doi: 10.18632/oncotarget.14489

Figure Lengend Snippet: Expression of miRNA-141 (A) Keap1 mRNA (B) and listed proteins ( B ) in RPEs transfected with antagomiR-141 or non-sense control antagomiR (“antagomiR-C”) were shown. Cells were also subjected to UV radiation (30 mJ/cm 2 ) and cultured for applied time, and cell viability was tested by MTT assay (C) Cell apoptosis was tested by Histone DNA ELISA assay (D) . “Transfection” indicates transfection reagents only. Protein expressions of Keap1, Nrf2, HO1 and NQO1 ( vs . β-actin) were quantified ( A and C ). For each assay, n=5. Experiments in this figure were repeated three times to insure consistency of results. * p <0.05 vs . “antagomiR-C” cells.

Article Snippet: The two non-overlapping lentiviral Nrf2 shRNAs, Nrf2 shRNA-A (sc-37030-V) and Nrf2 shRNA-B (sc-44332-V), along with lentiviral scramble control shRNA, were purchased from Santa Cruz Biotech (Santa Cruz); The lentiviral shRNA (10 μL/mL) was added directly to cultured cells for 36 hours.

Techniques: Expressing, Transfection, Control, Cell Culture, MTT Assay, Enzyme-linked Immunosorbent Assay

Journal: Oncotarget

Article Title: miRNA-141 attenuates UV-induced oxidative stress via activating Keap1-Nrf2 signaling in human retinal pigment epithelium cells and retinal ganglion cells

doi: 10.18632/oncotarget.14489

Figure Lengend Snippet: miRNA-141-expressing RPEs (“L1/2”) were infected with lentiviral Nrf2 shRNA (“shNrf2-A/-B”, with non-overlapping sequence) or scramble control shRNA (“shSCR”), expression of Nrf2 mRNA (A) and (B) and listed proteins (C) and (D) were shown. Above cells were also irradiated with UV (30 mJ/cm 2 ) and cultured 24 hours, and cell viability was tested by MTT assay (E) and (F) . Nrf2 expression ( vs . loading Erk1/2) was quantified ( C and D ). For each assay, n=5. Experiments in this figure were repeated three times to insure consistency of results. * p <0.05 vs . “shSCR” cells.

Article Snippet: The two non-overlapping lentiviral Nrf2 shRNAs, Nrf2 shRNA-A (sc-37030-V) and Nrf2 shRNA-B (sc-44332-V), along with lentiviral scramble control shRNA, were purchased from Santa Cruz Biotech (Santa Cruz); The lentiviral shRNA (10 μL/mL) was added directly to cultured cells for 36 hours.

Techniques: Expressing, Infection, shRNA, Sequencing, Control, Irradiation, Cell Culture, MTT Assay