Journal: bioRxiv

Article Title: Use of a human small airway epithelial cell line to study the interactions of Aspergillus fumigatus with pulmonary epithelial cells

doi: 10.1101/2023.04.18.537379

Figure Lengend Snippet: (A) Endocytosis of wild-type and Δ calA strains of A. fumigatus by A549 and HSAE cells. (B) Effects of anti-β1 integrin and anti-α5 integrin antibodies on the endocytosis of A. fumigatus Af293 by A549 and HSAE cells. (C) Western blots showing the deletion of integrin α5 (Int α5) in two different clones of A549 and HSAE cells. (D) Deletion of integrin α5 inhibits the endocytosis of A. fumigatus by A549 cells but not HSAE cells. (E) Effects of siRNA knockdown of E-cadherin on the endocytosis A. fumigatus Af293 by A549 and HSAE cells. (F) Effects of an anti-Dectin-1 antibody on the endocytosis of A. fumigatus Af293 by A549 and HSAE cells. Results in (A, B, D, E and F) are the mean ± SD of 3 independent experiments, each performed in triplicate; orgs/HPF, organisms per high-powered field; ns, not significant; ** P < 0.01, *** P < 0.001, **** P < 0.0001 by the unpaired Students t-test (A, E and F) and ANOVA with Dunnett’s test for multiple comparison (B and D).

Article Snippet: To knockdown E-cadherin, A549 and HSAE cells were transfected with E-cadherin siRNA (sc-35242; Santa Cruz Biotechnology), or random control siRNA (Qiagen) using Lipofectamine 2000 (Invitrogen) following the manufacturer’s instructions.

Techniques: Western Blot, Clone Assay, Knockdown, Comparison

Journal: bioRxiv

Article Title: Use of a human small airway epithelial cell line to study the interactions of Aspergillus fumigatus with pulmonary epithelial cells

doi: 10.1101/2023.04.18.537379

Figure Lengend Snippet: (A) Adherence of the indicated A. fumigatus strains to A549 and HSAE cells. (B) Effects of anti-β1 integrin and anti-α5 integrin antibodies on the adherence of A. fumigatus Af293 to A549 and HSAE cells. (C) Deletion of integrin α5 has no effect on the adherence of A. fumigatus Af293 to A549 and HSAE cells. (D) Effects of siRNA knockdown of E-cadherin on the adherence of A. fumigatus Af293 to A549 and HSAE cells. Top panels contain representative immunoblots of whole cell lysates showing siRNA knockdown of E-cadherin in A549 and HSAE cells. (E) Effects of anti-Dectin-1 antibody on the adherence of A. fumigatus Af293 to A549 and HSAE cells. Results are mean ± SD of 3 independent experiments, each performed in triplicate. orgs/HPF, organisms per high-powered field; ns, not significant; ** P < 0.01 by unpaired Students t-test (A, D and E) and ANOVA with Dunnett’s test for multiple comparison (B and C).

Article Snippet: To knockdown E-cadherin, A549 and HSAE cells were transfected with E-cadherin siRNA (sc-35242; Santa Cruz Biotechnology), or random control siRNA (Qiagen) using Lipofectamine 2000 (Invitrogen) following the manufacturer’s instructions.

Techniques: Knockdown, Western Blot, Comparison

Journal: Molecular cell

Article Title: Nucleus-translocated ACSS2 promotes the gene transcription for lysosomal biogenesis and autophagy

doi: 10.1016/j.molcel.2017.04.026

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: PX458 plasmid used for gene editing was obtained from Addgene ( Ran et al., 2013 ). pcDNA3.1-(HA) 2− TFE3 and pEGFP-C1-MITF plasmids are a gift of Nabeel Bardeesy from Harvard Medical School ( Perera et al., 2015 ). pEYFP-N1-TFEC plasmid is a gift of Richard Youle from National Institutes of Health ( Nezich et al., 2015 ).

Techniques: Transduction, Recombinant, Transfection, SYBR Green Assay, Chromatin Immunoprecipitation, Fractionation, Double Knockout, shRNA, Sequencing, Real-time Polymerase Chain Reaction, Plasmid Preparation, Software