Journal: Antimicrobial Agents and Chemotherapy

Article Title: Intra- and Extracellular Activities of Dicloxacillin against Staphylococcus aureus In Vivo and In Vitro ▿

doi: 10.1128/AAC.01400-09

Figure Lengend Snippet: In vitro intra- and extracellular regression data for the activity of DCX against S. aureus in concentration-kill studies (24-h drug exposure protocol)

Article Snippet: The initial intracellular inocula were (6.36 ± 4.41) × 10 5 CFU/ml for strain ATCC 25923 and (2.88 ± 1.72) × 10 5 CFU/ml for strain E19977 ( n = 6).

Techniques: In Vitro, Activity Assay, Concentration Assay

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Intra- and Extracellular Activities of Dicloxacillin against Staphylococcus aureus In Vivo and In Vitro ▿

doi: 10.1128/AAC.01400-09

Figure Lengend Snippet: In vitro time-kill studies of DCX against S. aureus at different fixed extracellular concentrations. (Left panels) Activity against the extracellular form of the bacteria measured in broth; (right panels) activity against the intracellular form of the bacteria measured in THP-1 macrophages. The activity was measured against two different MSSA strains (upper panels, ATCC 25923 [MIC = 0.125 mg/liter]; lower panels, E19977 [MIC = 0.5 mg/liter]). The graphs show the activity as changes in the numbers of CFU (Δ log CFU; means ± standard deviations; n = 3). The change in the numbers of CFU is measured as the numbers of CFU/ml in the extracellular studies and the numbers of CFU/mg cell protein in the intracellular studies.

Article Snippet: The initial intracellular inocula were (6.36 ± 4.41) × 10 5 CFU/ml for strain ATCC 25923 and (2.88 ± 1.72) × 10 5 CFU/ml for strain E19977 ( n = 6).

Techniques: In Vitro, Activity Assay, Bacteria

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Intra- and Extracellular Activities of Dicloxacillin against Staphylococcus aureus In Vivo and In Vitro ▿

doi: 10.1128/AAC.01400-09

Figure Lengend Snippet: In vitro dose-response curves of DCX against extracellular (measured in broth) and intracellular (measured in THP-1 cells) S. aureus (strains ATCC 25923 and E19977). The graphs show the activity as changes in the numbers of CFU (Δ log CFU; means ± standard deviations; n = 3). The change in the numbers of CFU is measured as the numbers of CFU per ml broth in the extracellular studies and as the numbers of CFU per mg cell protein in the intracellular studies. The initial inocula for the extracellular study (time zero) were (1.78 ± 0.34) × 106 CFU/ml for strain ATCC 25923 and (1.23 ± 0.25) × 106 CFU/ml for strain E19977 (n = 3). The initial (postphagocytosis) inocula for the intracellular study (time zero) were (7.14 ± 2.59) × 106 CFU/mg protein for strain ATCC 25923 and (9.69 ± 1.23) × 106 CFU/mg protein for strain E19977 (n = 3). The sigmoidal function was used for fitting (see Table ​Table22 for goodness-of-fit and regression parameters). (A) Comparison of the intra- and extracellular activities against each strain versus the extracellular concentration (mg/liter); (B) comparison of the extracellular and intracellular activities against the two strains versus equipotent extracellular concentrations (multiples of the MICs).

Article Snippet: The initial intracellular inocula were (6.36 ± 4.41) × 10 5 CFU/ml for strain ATCC 25923 and (2.88 ± 1.72) × 10 5 CFU/ml for strain E19977 ( n = 6).

Techniques: In Vitro, Activity Assay, Comparison, Concentration Assay

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Intra- and Extracellular Activities of Dicloxacillin against Staphylococcus aureus In Vivo and In Vitro ▿

doi: 10.1128/AAC.01400-09

Figure Lengend Snippet: In vivo dose-response curves after single doses of dicloxacillin against S. aureus (strains ATCC 25923 and E19977) measured in a mouse peritonitis model (4-h drug exposure). The graphs displays (i) the total activity (gray circles and solid line), (ii) the activity considered to be extracellular (open squares and dashed line), and (iii) the activity considered to be intracellular (closed triangles and dotted line). The activity is displayed as changes in the numbers of CFU (Δ log CFU), with each datum point corresponding to one animal. The initial total inocula were (9.80 ± 4.85) × 105 CFU/ml for strain ATCC 25923 and (6.26 ± 2.87) × 105 CFU/ml for strain E19977 (n = 6). The initial extracellular inocula were (4.26 ± 3.03) × 104 CFU/ml for strain ATCC 25923 and (3.03 ± 0.85) × 104 CFU/ml for strain E19977 (n = 6). The initial intracellular inocula were (6.36 ± 4.41) × 105 CFU/ml for strain ATCC 25923 and (2.88 ± 1.72) × 105 CFU/ml for strain E19977 (n = 6). The sigmoidal function was used for fitting (see Table ​Table44 for the goodness-of-fit and regression parameters).

Article Snippet: The initial intracellular inocula were (6.36 ± 4.41) × 10 5 CFU/ml for strain ATCC 25923 and (2.88 ± 1.72) × 10 5 CFU/ml for strain E19977 ( n = 6).

Techniques: In Vivo, Activity Assay

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Intra- and Extracellular Activities of Dicloxacillin against Staphylococcus aureus In Vivo and In Vitro ▿

doi: 10.1128/AAC.01400-09

Figure Lengend Snippet: In vivo intra- and extracellular regression data for dose-kill studies of activity of DCX against S. aureus (4-h treatment protocol)

Article Snippet: The initial intracellular inocula were (6.36 ± 4.41) × 10 5 CFU/ml for strain ATCC 25923 and (2.88 ± 1.72) × 10 5 CFU/ml for strain E19977 ( n = 6).

Techniques: In Vivo, Activity Assay

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Intra- and Extracellular Activities of Dicloxacillin against Staphylococcus aureus In Vivo and In Vitro ▿

doi: 10.1128/AAC.01400-09

Figure Lengend Snippet: Relationship between fTMIC, fAUC/MIC, fCmax/MIC, and the total 24-h dose for the 24-h accumulated amount of free drug and the efficacy of DCX against MSSA strain E19977, as measured in the mouse peritonitis model (24 h of drug exposure). See Table ​Table11 for the dosing regimens applied. The graphs display (i) the total activity (left panels, gray circles), (ii) the activity considered to be against extracellular bacteria (middle panels, open squares), and (iii) the activity considered to be against intracellular bacteria (right panels, closed triangles). The activity is displayed as changes in the numbers of CFU (Δ log CFU), with each datum point corresponding to one animal. The initial total, extracellular, and intracellular inocula were (5.61 ± 0.15) × 105, (2.12 ± 1.51) × 104, (2.86 ± 2.81) × 105 CFU/ml, respectively (n = 8). The sigmoidal function was used, and R2 represents the goodness of fit.

Article Snippet: The initial intracellular inocula were (6.36 ± 4.41) × 10 5 CFU/ml for strain ATCC 25923 and (2.88 ± 1.72) × 10 5 CFU/ml for strain E19977 ( n = 6).

Techniques: Activity Assay, Bacteria