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Image Search Results
Journal: The AAPS Journal
Article Title: Development of Lipidoid Nanoparticles for siRNA Delivery to Neural Cells
doi: 10.1208/s12248-021-00653-2
Figure Lengend Snippet: C12-200 LNPs are cytocompatible with TRPV1-expressing breast cancer cell line panel ( a ), rat primary DRG neurons ( b ), and MDA-MB-231 cells ( c ). Cells were incubated with LNPs containing 50 nM siGAPDH ( a ), 50 nM siTRPV1 ( b ), and the indicated concentrations of siGFP-LNPs ( c ) for 4 h. Untreated cells and cells treated with PEI (50 μg/mL) were used as negative and positive controls, respectively. Cell viability was determined 24 h post-transfection using a CellTiter-Glo luminescence viability assay, and the data were normalized to untreated cells. Data represents mean + SD ( n = 6)
Article Snippet: Human cortical neuron cell line (HCN-2) (CRL-10742) and primary rat cortical neurons (RCN) (A10840-02) were purchased from
Techniques: Expressing, Incubation, Transfection, Viability Assay