Journal:

Article Title: Unconventional Genomic Organization in the Alpha Subgroup of the Proteobacteria

doi:

Figure Lengend Snippet: Strain designations, number and sizes of replicons, and estimated genome sizes for organisms belonging to the family Rhizobiaceae

Article Snippet: Lanes: 1, Schizosaccharomyces pombe ; 2, Rhodobacter capsulatus ATCC 11166; 3, P. myrsinacearum ATCC 43590 T ; 4, O. anthropi ATCC 49188 T ; 5, O. anthropi LMG 3301; 6, M. dimorpha ATCC 4279 T ; 7, Rhizobium meliloti 2011; 8, A. tumefaciens C58; 9, H. wingei .

Techniques:

Journal: iScience

Article Title: Lattice-patterned collagen fibers and their dynamics in axolotl skin regeneration

doi: 10.1016/j.isci.2022.104524

Figure Lengend Snippet:

Article Snippet: Rabbit monoclonal anti-mCherry (E5D8F) , Cell Signaling Technology , Cat#43590; RRID: AB_2799246.

Techniques: Staining, Recombinant, Software

Journal: Journal of Fungi

Article Title: Differentiation of Basidiobolus spp. Isolates: RFLP of a Diagnostic PCR Amplicon Matches Sequence-Based Classification and Growth Temperature Preferences

doi: 10.3390/jof7020110

Figure Lengend Snippet: Origin of Basidiobolus isolates used in the present study and GenBank accession numbers of the submitted 18S rRNA (SSU) and partial 28S rRNA (LSU, D1/D2 domain) gene sequences.

Article Snippet: Bm DSM3120 , SSU: MW127176 , , , Culture collection DSMZ, Germany , Type strain.

Techniques:

Journal: Oncotarget

Article Title: Dimerization of EGFR and HER2 induces breast cancer cell motility through STAT1-dependent ACTA2 induction

doi: 10.18632/oncotarget.10843

Figure Lengend Snippet: A . The levels of p-HER2 (pT877), t-HER2, and t-EGFR expression were analyzed by western blots. ACTA2 and STAT1 mRNA expression were analyzed by real-time PCR. EGFR and HER2 expression were analyzed by confocal microscopy. B . ACTA2 and STAT1 expression patterns were analyzed by cDNA microarray analysis. C . Heatmap of ACTA2 and STAT1 expression in tumors from breast cancer patients generated using R statistical software. D . ACTA2, p-STAT1 (pS727), t-STAT1, and β-actin expression analyzed by western blots. ACTA2 and STAT1 mRNA expression were analyzed by real-time PCR. ACTA2 expression was analyzed by confocal microscopy. Results are representative of three independent experiments. Values are means ± SEM. * p < 0.05, ** p < 0.01 vs. Vec. Vec; empty vector.

Article Snippet: Empty and ACTA2 shRNA were from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Techniques: Expressing, Western Blot, Real-time Polymerase Chain Reaction, Confocal Microscopy, Microarray, Generated, Software, Plasmid Preparation

Journal: Oncotarget

Article Title: Dimerization of EGFR and HER2 induces breast cancer cell motility through STAT1-dependent ACTA2 induction

doi: 10.18632/oncotarget.10843

Figure Lengend Snippet: A . HEK293 cells were transiently transfected with vec or CA-HER2 for 48 h. B-D . HER2-overexpressing MDA-MB231, SKBR3, and BT474 cells were transiently transfected with scrambled or siHER2 for 48 h. The levels of ACTA2, p-HER2 (pT877), t-HER2, p-STAT1 (pS727), t-STAT1, and β-actin were analyzed by western blots. ACTA2 mRNA was analyzed by real-time PCR. Results are representative of three independent experiments. Values are means ± SEM. * p < 0.05, ** p < 0.01 vs. Vec or scrambled. Vec; empty vector.

Article Snippet: Empty and ACTA2 shRNA were from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Techniques: Transfection, Western Blot, Real-time Polymerase Chain Reaction, Plasmid Preparation

Journal: Oncotarget

Article Title: Dimerization of EGFR and HER2 induces breast cancer cell motility through STAT1-dependent ACTA2 induction

doi: 10.18632/oncotarget.10843

Figure Lengend Snippet: A, B . After serum starvation for 24 h, HER2-overexpressing MDA-MB231 cells were treated with 10 μM piceatannol, AG490, WHI-P-154, or fludarabine, for 24 h. C . HER2-overexpressing MDA-MB231 cells were transiently transfected with scrambled or siSTAT1 for 48 h. D . HEK293 cells were transiently transfected with vec or STAT1 for 48 h. ACTA2, p-STAT1 (pS727), t-STAT1, and β-actin expression was analyzed by western blots. ACTA2 mRNA was analyzed by real-time PCR. Results are representative of three independent experiments. Values are means ± SEM. * p < 0.05, ** p < 0.01 vs. (-) control, scrambled, or Vec. Vec; empty vector.

Article Snippet: Empty and ACTA2 shRNA were from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Techniques: Transfection, Expressing, Western Blot, Real-time Polymerase Chain Reaction, Control, Plasmid Preparation

Journal: Oncotarget

Article Title: Dimerization of EGFR and HER2 induces breast cancer cell motility through STAT1-dependent ACTA2 induction

doi: 10.18632/oncotarget.10843

Figure Lengend Snippet: The clinical value of ACTA2, STAT1, and HER2 was analyzed by SurvExpress software ( http://bioinformatica.mty.itesm.mx/SurvExpress ). A . Kaplan-Meier curves (relapse-free survival). B . Heatmap. C . Box plots.

Article Snippet: Empty and ACTA2 shRNA were from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Techniques: Software

Journal: Oncotarget

Article Title: Dimerization of EGFR and HER2 induces breast cancer cell motility through STAT1-dependent ACTA2 induction

doi: 10.18632/oncotarget.10843

Figure Lengend Snippet: MDA-MB231 HER2 A-C . and 4T1 mammary carcinoma cells D-F . were stably transfected with vec or ACTA2 shRNA for 48 h and selected with puromycin. (A, D) Migrating ability of cells overexpressing scrambled or ACTA2 shRNA analyzed by wound healing assays. (B, E) Invasiveness of cells with vec or ACTA2 shRNA was analyzed by Boyden chamber assays. (C, F) ACTA2 and β-actin expression was analyzed by western blots. ACTA2 mRNA was analyzed by real-time PCR. Results are representative of three independent experiments. Values are means ± SEM. ** p < 0.01 vs. Vec. Vec; empty vector.

Article Snippet: Empty and ACTA2 shRNA were from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Techniques: Stable Transfection, Transfection, shRNA, Expressing, Western Blot, Real-time Polymerase Chain Reaction, Plasmid Preparation

Journal: Oncotarget

Article Title: Dimerization of EGFR and HER2 induces breast cancer cell motility through STAT1-dependent ACTA2 induction

doi: 10.18632/oncotarget.10843

Figure Lengend Snippet: Secondary mammary fat pads of mice were injected with 4T1 mammary carcinoma cells overexpressing vec or ACTA2 shRNA. A . After 24 days, lung tissues were removed, divided into vec (n = 5) or ACTA2-knockdown (n = 5) groups and analyzed for metastatic events. White arrow head, metastatic nodules. The number of metastatic nodules was counted in lung tissues. B . PCNA expression and tumor volume were measured to determine tumor growth. C . The levels of ACTA2 and β-actin expression were analyzed by western blots. ACTA2 mRNA was analyzed by real-time PCR. Values are means ± SEM. ** p < 0.01 vs. Vec.

Article Snippet: Empty and ACTA2 shRNA were from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Techniques: Injection, shRNA, Knockdown, Expressing, Western Blot, Real-time Polymerase Chain Reaction

Journal: Oncotarget

Article Title: Dimerization of EGFR and HER2 induces breast cancer cell motility through STAT1-dependent ACTA2 induction

doi: 10.18632/oncotarget.10843

Figure Lengend Snippet: Specific primer sequences for analysis of EGFR, HER2, ACTA2, STAT1, and β-actin mRNA expression

Article Snippet: Empty and ACTA2 shRNA were from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Techniques: