Journal: Biotechnology and bioengineering

Article Title: A novel aryl acylamidase from Nocardia farcinica hydrolyses polyamide.

doi: 10.1002/bit.22139

Figure Lengend Snippet: Figure 1. Native gel electrophoresis of the polyamidase from Nocardia farcinica after size exclusion chromatography (fraction 20 and 21). Protein bands were stained with silver. [Color figure can be seen in the online version of this article, available at www.interscience.wiley.com.]

Article Snippet: This strain was identified as N. farcinica at DSMZ culture collection (German Collection of Microorganisms and Cell Cultures) using standard methods including 16S rRNA comparison, morphology, physiology, fatty acid pattern and the length of mycolic acids and is deposited under the number 05-253.

Techniques: Nucleic Acid Electrophoresis, Size-exclusion Chromatography, Staining

Journal: Biotechnology and bioengineering

Article Title: A novel aryl acylamidase from Nocardia farcinica hydrolyses polyamide.

doi: 10.1002/bit.22139

Figure Lengend Snippet: Figure 2. Sequence alignment of the 6-aminohexanoate-cyclic dimer hydrolase (EI) from Arthrobacter sp. KI72 (formerly Flavobacterium; BAA05090), the peptide amidase (Pam) from Stenotrophomonas maltophilia (CAC93616), with a putative amidase from Nocardia farcinica (YP_119043). The catalytic triad characteristic for amidase signature enzymes is framed, highly conserved regions are marked in gray and peptide fragments of the polyamidase from Nocardia farcinica (this study) matching with the putative amidase from Nocardia farcinica (YP_119043) are underlined. GenBank accession numbers are given in brackets.

Article Snippet: This strain was identified as N. farcinica at DSMZ culture collection (German Collection of Microorganisms and Cell Cultures) using standard methods including 16S rRNA comparison, morphology, physiology, fatty acid pattern and the length of mycolic acids and is deposited under the number 05-253.

Techniques: Sequencing

Journal: Biotechnology and bioengineering

Article Title: A novel aryl acylamidase from Nocardia farcinica hydrolyses polyamide.

doi: 10.1002/bit.22139

Figure Lengend Snippet: Figure 3. Phylogenetic tree based on amino acid sequences of the polyamidase from Nocardia farcinica (fragments as identified by MS) and other highly homologous bacterial amidases as well as amidases with substrate specificity for 6-aminohexanoate oligomers. The alignment was created using the Clustal W algorithm (Thompson et al., 1994). Tree calculation was based on a sequence distance method and utilized the Neighbor Joining (NJ) algorithm (Saitou and Nei, 1987). Accession numbers given refer to GenBank while Arthrobacter sp. was formerly named Flavobacterium. [Color figure can be seen in the online version of this article, available at www.interscience.wiley.com.]

Article Snippet: This strain was identified as N. farcinica at DSMZ culture collection (German Collection of Microorganisms and Cell Cultures) using standard methods including 16S rRNA comparison, morphology, physiology, fatty acid pattern and the length of mycolic acids and is deposited under the number 05-253.

Techniques: Sequencing

Journal: Biotechnology and bioengineering

Article Title: A novel aryl acylamidase from Nocardia farcinica hydrolyses polyamide.

doi: 10.1002/bit.22139

Figure Lengend Snippet: Figure 4. Increase of rising height of polyamide 6 fabrics after treatment with a polyamidase from N. farcinica compared to controls containing enzyme inhibitor and compared to the effect of silicone based finisher (‘‘chemical’’). Data represent the average of three measurements.

Article Snippet: This strain was identified as N. farcinica at DSMZ culture collection (German Collection of Microorganisms and Cell Cultures) using standard methods including 16S rRNA comparison, morphology, physiology, fatty acid pattern and the length of mycolic acids and is deposited under the number 05-253.

Techniques: