43-166P Search Results


phalloidin  (Thermo Fisher)
90
Thermo Fisher phalloidin
Vangl2 deletion in the uterine epithelium contribute to aberrant implantation and decidualization. a Day 5 implantation sites (blue bands) in Vangl2 f/f and Vangl2 f/f Ltf Cre/+ females and arrows indicate weak blue bands. b Histology of day 5 implantation sites in Vangl2 f/f and Vangl2 f/f Ltf Cre/+ mice. * embryo. Scale bar: 200 μm. c In situ hybridization of Ptgs2 and Bmp2 in Vangl2 f/f and Vangl2 f/f Ltf Cre/+ implantation sites on day 5. Scale bar: 200 μm. Arrowheads indicate the location of embryos. d 3D reconstruction of Scrib and <t>Phalloidin</t> localization at the apical surface of the epithelium by IF in Vangl2 f/f and Vangl2 f/f Ltf Cre/+ uterine luminal epithelium (LE) on day 4. Scale bar: 10 μm. e Cell shape analysis of uterine epithelial cells using SEGGA software. f , g Quantification of cell eccentricity and the long-to-short axis ratio in Vangl2 f/f and Vangl2 f/f Ltf Cre epithelial cells (Mean ± s.e.m. is derived from the indicated number of samples and analyzed by Student’s t- test, *** p < 0.001). h IF of Caspase3 and CK8 in day 6 implantation sites of Vangl2 f/f and Vangl2 f/f Ltf Cre/+ mice. Scale bar: 200 μm. i IF of CDH1 and CTNNB1 in day 6 implantation sites of Vangl2 f/f and Vangl2 f/f Ltf Cre/+ mice. Scale bar: 200 μm. All images are representative of three independent experiments
Phalloidin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/phalloidin/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
phalloidin - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
tbc1d25 crispr activation plasmid  (Santa Cruz Biotechnology)
N/A
CRISPR Cas9 KO Plasmids consists of TBC1D25 specific 20 nt guide RNA sequences derived from the GeCKO v2 library For CRISPR gene knockout gRNA sequences direct the Cas9 protein to induce a site specific double
   Buy from Supplier
otoa nm 001161683 human over expression lysate  (OriGene)
N/A
OTOA HEK293T cell transient overexpression lysate as WB positive control
   Buy from Supplier
gpr139 double nickase plasmid  (Santa Cruz Biotechnology)
N/A
CRISPR Cas9 KO Plasmids consists of GPR139 specific 20 nt guide RNA sequences derived from the GeCKO v2 library For CRISPR gene knockout gRNA sequences direct the Cas9 protein to induce a site specific double
   Buy from Supplier
β3gn tl1 hdr plasmid  (Santa Cruz Biotechnology)
N/A
CRISPR Cas9 KO Plasmids consists of β3Gn TL1 specific 20 nt guide RNA sequences derived from the GeCKO v2 library For CRISPR gene knockout gRNA sequences direct the Cas9 protein to induce a site specific
   Buy from Supplier
(2s)-2-amino-2-(1h-pyrrol-3-yl)ethan-1-ol  (Biosynth Carbosynth)
N/A
   Buy from Supplier
2,5-dibromo-3-methoxypyrazine  (Biosynth Carbosynth)
N/A
   Buy from Supplier
dennd2a hdr plasmid  (Santa Cruz Biotechnology)
N/A
CRISPR Cas9 KO Plasmids consists of DENND2A specific 20 nt guide RNA sequences derived from the GeCKO v2 library For CRISPR gene knockout gRNA sequences direct the Cas9 protein to induce a site specific double
   Buy from Supplier
boc-arg(mtr)-oh  (Biosynth Carbosynth)
N/A
   Buy from Supplier
slc35c2 cytosection  (OriGene)
N/A
Transient overexpression of SLC35C2 NM 173073 in HEK293T cells paraffin embedded 4 um sections controls for ICC IHC staining 25 slides per pack
   Buy from Supplier
kctd11 antibody  (Abbexa)
N/A
KCTD11 Antibody is a Goat Polyclonal antibody against KCTD11
   Buy from Supplier
i tagt plasmid 43166  (Addgene inc)
N/A
Standard format Plasmid sent in bacteria as agar stab
   Buy from Supplier

Image Search Results


Vangl2 deletion in the uterine epithelium contribute to aberrant implantation and decidualization. a Day 5 implantation sites (blue bands) in Vangl2 f/f and Vangl2 f/f Ltf Cre/+ females and arrows indicate weak blue bands. b Histology of day 5 implantation sites in Vangl2 f/f and Vangl2 f/f Ltf Cre/+ mice. * embryo. Scale bar: 200 μm. c In situ hybridization of Ptgs2 and Bmp2 in Vangl2 f/f and Vangl2 f/f Ltf Cre/+ implantation sites on day 5. Scale bar: 200 μm. Arrowheads indicate the location of embryos. d 3D reconstruction of Scrib and Phalloidin localization at the apical surface of the epithelium by IF in Vangl2 f/f and Vangl2 f/f Ltf Cre/+ uterine luminal epithelium (LE) on day 4. Scale bar: 10 μm. e Cell shape analysis of uterine epithelial cells using SEGGA software. f , g Quantification of cell eccentricity and the long-to-short axis ratio in Vangl2 f/f and Vangl2 f/f Ltf Cre epithelial cells (Mean ± s.e.m. is derived from the indicated number of samples and analyzed by Student’s t- test, *** p < 0.001). h IF of Caspase3 and CK8 in day 6 implantation sites of Vangl2 f/f and Vangl2 f/f Ltf Cre/+ mice. Scale bar: 200 μm. i IF of CDH1 and CTNNB1 in day 6 implantation sites of Vangl2 f/f and Vangl2 f/f Ltf Cre/+ mice. Scale bar: 200 μm. All images are representative of three independent experiments

Journal: Nature Communications

Article Title: Tridimensional visualization reveals direct communication between the embryo and glands critical for implantation

doi: 10.1038/s41467-018-03092-4

Figure Lengend Snippet: Vangl2 deletion in the uterine epithelium contribute to aberrant implantation and decidualization. a Day 5 implantation sites (blue bands) in Vangl2 f/f and Vangl2 f/f Ltf Cre/+ females and arrows indicate weak blue bands. b Histology of day 5 implantation sites in Vangl2 f/f and Vangl2 f/f Ltf Cre/+ mice. * embryo. Scale bar: 200 μm. c In situ hybridization of Ptgs2 and Bmp2 in Vangl2 f/f and Vangl2 f/f Ltf Cre/+ implantation sites on day 5. Scale bar: 200 μm. Arrowheads indicate the location of embryos. d 3D reconstruction of Scrib and Phalloidin localization at the apical surface of the epithelium by IF in Vangl2 f/f and Vangl2 f/f Ltf Cre/+ uterine luminal epithelium (LE) on day 4. Scale bar: 10 μm. e Cell shape analysis of uterine epithelial cells using SEGGA software. f , g Quantification of cell eccentricity and the long-to-short axis ratio in Vangl2 f/f and Vangl2 f/f Ltf Cre epithelial cells (Mean ± s.e.m. is derived from the indicated number of samples and analyzed by Student’s t- test, *** p < 0.001). h IF of Caspase3 and CK8 in day 6 implantation sites of Vangl2 f/f and Vangl2 f/f Ltf Cre/+ mice. Scale bar: 200 μm. i IF of CDH1 and CTNNB1 in day 6 implantation sites of Vangl2 f/f and Vangl2 f/f Ltf Cre/+ mice. Scale bar: 200 μm. All images are representative of three independent experiments

Article Snippet: IF for ESR1 (1:300, sc-542; Santa Cruz), CDH1 (1:300, sc-31020, Santa Cruz), Scribble (1:1000, H300, Santa Cruz), CTNNB1 (1:500, sc-1496, Santa Cruz), PGR (1:300, 8757; Cell Signaling technology), rabbit CDH1 (1:300, 3195s, Cell Signaling Technology), Vangl2 (1:10,000, custom made in JP Borg’s laboratory, INSERM), ZO-1 (1:300, 33–9100, Invitrogen), phalloidin (1:1000, 43166A, Thermo Scientific), FOXA2 (1:300, WRAB-FOXA2, Seven Hills Bioreagents), CK8 (1:1000, TROMA-1, Hybridoma Bank, Iowa), and Caspase 3 (1:300, 9661s, Cell Signaling technology) was performed using secondary antibodies conjugated with Cy-2, Cy-3, Alexa 488, or Alexa 594 (1:300, Jackson Immuno Research).

Techniques: In Situ Hybridization, Software, Derivative Assay