Journal: Clinical cancer research : an official journal of the American Association for Cancer Research

Article Title: Reciprocal modifications of CLIC4 in tumor epithelium and stroma mark malignant progression of multiple human cancers.

doi: 10.1158/1078-0432.CCR-06-1562

Figure Lengend Snippet: Fig. 2. CLIC4 expression and subcellular localization are altered in human tumors. Human normal and matched tumor (roman numerals, tumor stage) tissue sections representing (A) esophagus, (B) kidney, and (C) colon from the tissue microarrays are immunostained with anti-CLIC4 antibody and visualized by bright-field microscopy under 40 magnification. Inset, lower magnification (10). Black arrows, CLIC4 localized to the nucleus in the normal tissues. Similar staining patterns of CLIC4 were found in multiple human tumor types. Representative immunohistochemical stainings.

Article Snippet: Immunohistochemistry, immunofluorescence, and tissue microarray staining Human tissue microarrays were obtained from multiple sources: TARP tissue arrays (National Cancer Institute), ‘‘matched’’ human tumor tissue arrays (Imgenex, San Diego, CA), Food and Drug Administration–standard normal and tumor tissue arrays (Biochain, Hayward, CA) and human cancer screen tissue arrays (Clinomics), prostate tumor arrays (Baylor Specialized Programs of Research Excellence), and human tissue arrays (Accumax) were used for CLIC4 expression and localization studies.

Techniques: Expressing, Microscopy, Staining, Immunohistochemical staining

Journal: BioMed Research International

Article Title: Propolis Modulates Fibronectin Expression in the Matrix of Thermal Injury

doi: 10.1155/2014/748101

Figure Lengend Snippet: Extractability of FN components isolated from wounds treated with NaCl, SSD, propolis, and propolis vehicle and reacting with reagents against fibronectin.

Article Snippet: The following antibodies were used: monoclonal mouse anti-human fibronectin antibody number 42042, purchased from QED Bioscience Inc., San Diego, California, USA, and goat anti-rabbit immunoglobulin G number A5420, conjugated with horseradish peroxidase, obtained from Sigma-Aldrich, Germany.

Techniques: Isolation

Journal: BioMed Research International

Article Title: Propolis Modulates Fibronectin Expression in the Matrix of Thermal Injury

doi: 10.1155/2014/748101

Figure Lengend Snippet: Immunoreactivity assessment of extract components of burn wounds with antibodies against FN. The extract components after electrophoretic separations in gradient polyacrylamide gel 4–15% were subjected to electrotransfer on the membrane Immobilon P and next to the reaction with the antibodies by Western blotting method; lane 1, molecular mass markers, lanes 2, 3, 4, and 5, components released on the 5th day of healing from wounds treated with NaCl, SSD, propolis, and propolis vehicle. The arrows indicate the migration position of molecules of known molecular masses, while the stars indicate the position of fibronectin monomers.

Article Snippet: The following antibodies were used: monoclonal mouse anti-human fibronectin antibody number 42042, purchased from QED Bioscience Inc., San Diego, California, USA, and goat anti-rabbit immunoglobulin G number A5420, conjugated with horseradish peroxidase, obtained from Sigma-Aldrich, Germany.

Techniques: Electrotransfer, Membrane, Western Blot, Migration