42039 Search Results


aspergillus versicolor atcc 42039  (ATCC)
90
ATCC aspergillus versicolor atcc 42039
Aspergillus Versicolor Atcc 42039, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/aspergillus versicolor atcc 42039/product/ATCC
Average 90 stars, based on 1 article reviews
aspergillus versicolor atcc 42039 - by Bioz Stars, 2026-06
90/100 stars
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naip1  (Santa Cruz Biotechnology)
90
Santa Cruz Biotechnology naip1
Fig. 4. hNAIP-NLRC4 sensing of PscI-elicited human macrophages and PBMCs. Human monocyte cell line THP-1 (A–D) was differentiated into macrophages with PMA, and PBMCs (E) were isolated from peripheral venous blood. (A, B) Cells were seeded in 96-well culture plates and treated with fresh medium or 500 ng per well of ligands PscI, PscF, PopB, PrgI and MSU or 107 freeze-dried HKLM per well. Prior to treat- ment, cells were either left untreated or treated with the NLRP3 inhibitor Bay11-7082. The cytotoxicity (A) and the secretion of IL-1β (B) were determined 24 h post-contact by LDH release and ELISA. THP-1 cells (C, D) or PBMCs (E) were seeded in 24-well culture plates and were then transfected with <t>NAIP1,</t> NLRC4 or control siRNA. PscI at 500 ng or control medium were added per well. Relative gene expression was determined, 2 h post-contact, by quantitative RT–PCR (C); cytotoxicity and IL-1β secretion were determined by LDH release and ELISA (D, E). *P < 0.05; **P < 0.01; ***P < 0.001 compared to control.
Naip1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/naip1/product/Santa Cruz Biotechnology
Average 90 stars, based on 1 article reviews
naip1 - by Bioz Stars, 2026-06
90/100 stars
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tissue microarray  (Novus Biologicals)
92
Novus Biologicals tissue microarray
Fig. 4. hNAIP-NLRC4 sensing of PscI-elicited human macrophages and PBMCs. Human monocyte cell line THP-1 (A–D) was differentiated into macrophages with PMA, and PBMCs (E) were isolated from peripheral venous blood. (A, B) Cells were seeded in 96-well culture plates and treated with fresh medium or 500 ng per well of ligands PscI, PscF, PopB, PrgI and MSU or 107 freeze-dried HKLM per well. Prior to treat- ment, cells were either left untreated or treated with the NLRP3 inhibitor Bay11-7082. The cytotoxicity (A) and the secretion of IL-1β (B) were determined 24 h post-contact by LDH release and ELISA. THP-1 cells (C, D) or PBMCs (E) were seeded in 24-well culture plates and were then transfected with <t>NAIP1,</t> NLRC4 or control siRNA. PscI at 500 ng or control medium were added per well. Relative gene expression was determined, 2 h post-contact, by quantitative RT–PCR (C); cytotoxicity and IL-1β secretion were determined by LDH release and ELISA (D, E). *P < 0.05; **P < 0.01; ***P < 0.001 compared to control.
Tissue Microarray, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tissue microarray/product/Novus Biologicals
Average 92 stars, based on 1 article reviews
tissue microarray - by Bioz Stars, 2026-06
92/100 stars
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pmyc-gfp-tnrc6a-del.gw-i+ii-nes-mut (plasmid #42039)  (Addgene inc)
N/A
Standard format: Plasmid sent in bacteria as agar stab
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Image Search Results


Fig. 4. hNAIP-NLRC4 sensing of PscI-elicited human macrophages and PBMCs. Human monocyte cell line THP-1 (A–D) was differentiated into macrophages with PMA, and PBMCs (E) were isolated from peripheral venous blood. (A, B) Cells were seeded in 96-well culture plates and treated with fresh medium or 500 ng per well of ligands PscI, PscF, PopB, PrgI and MSU or 107 freeze-dried HKLM per well. Prior to treat- ment, cells were either left untreated or treated with the NLRP3 inhibitor Bay11-7082. The cytotoxicity (A) and the secretion of IL-1β (B) were determined 24 h post-contact by LDH release and ELISA. THP-1 cells (C, D) or PBMCs (E) were seeded in 24-well culture plates and were then transfected with NAIP1, NLRC4 or control siRNA. PscI at 500 ng or control medium were added per well. Relative gene expression was determined, 2 h post-contact, by quantitative RT–PCR (C); cytotoxicity and IL-1β secretion were determined by LDH release and ELISA (D, E). *P < 0.05; **P < 0.01; ***P < 0.001 compared to control.

Journal: International immunology

Article Title: The human NAIP-NLRC4-inflammasome senses the Pseudomonas aeruginosa T3SS inner-rod protein.

doi: 10.1093/intimm/dxx047

Figure Lengend Snippet: Fig. 4. hNAIP-NLRC4 sensing of PscI-elicited human macrophages and PBMCs. Human monocyte cell line THP-1 (A–D) was differentiated into macrophages with PMA, and PBMCs (E) were isolated from peripheral venous blood. (A, B) Cells were seeded in 96-well culture plates and treated with fresh medium or 500 ng per well of ligands PscI, PscF, PopB, PrgI and MSU or 107 freeze-dried HKLM per well. Prior to treat- ment, cells were either left untreated or treated with the NLRP3 inhibitor Bay11-7082. The cytotoxicity (A) and the secretion of IL-1β (B) were determined 24 h post-contact by LDH release and ELISA. THP-1 cells (C, D) or PBMCs (E) were seeded in 24-well culture plates and were then transfected with NAIP1, NLRC4 or control siRNA. PscI at 500 ng or control medium were added per well. Relative gene expression was determined, 2 h post-contact, by quantitative RT–PCR (C); cytotoxicity and IL-1β secretion were determined by LDH release and ELISA (D, E). *P < 0.05; **P < 0.01; ***P < 0.001 compared to control.

Article Snippet: Inhibitors were used following the manufacturer’s instructions. siRNA transfection For siRNAs knockdown, THP-1 cells were differentiated in 24-well plates (106 cells per well). siRNAs specific for NLRC4 and NAIP1 were transfected into macrophages using a siRNA Reagent System (sc-45064, Santa Cruz Biotechnology, Santa Cruz, CA, USA) according to the manufacturer’s instructions. siRNAs were purchased from Santa Cruz Biotechnology.

Techniques: Isolation, Enzyme-linked Immunosorbent Assay, Transfection, Control, Gene Expression, Quantitative RT-PCR