Journal: International Journal of Molecular Sciences

Article Title: Antiviral Effects of ABMA and DABMA against Influenza Virus In Vitro and In Vivo via Regulating the Endolysosomal Pathway and Autophagy

doi: 10.3390/ijms23073940

Figure Lengend Snippet: Cytotoxicity and antiviral activity of compounds against influenza virus infection.

Article Snippet: Oseltamivir phosphate, 3-methyladenine (3-MA), and Bafilomycin A1 (Baf A1) were from MCE (Shanghai, China).

Techniques: Activity Assay, Virus, Infection

Journal: Dose-Response

Article Title: Biochemical Investigation of Inhibitory Activities of Plant-Derived Bioactive Compounds Against Carbohydrate and Glucagon-Like Peptide-1 Metabolizing Enzymes

doi: 10.1177/15593258221093275

Figure Lengend Snippet: Chemical structures of selected ligands for molecular docking analysis. (A) Chemical structures of tested compounds taxifolin (TAX) and resveratrol (RSV). (B) 2D-structural representation of standard compounds acarbose (ACB), miglitol (MGL), and diprotin (DPT). (C) Supposed conformation of selected hits and standard compound in their corresponding molecular targets.

Article Snippet: Resveratrol (CHEM-IMPEX INT’L INC), taxifolin (Sigma aldrich), acarbose (Carbosnyth,USA), diprotin (Sigma aldrich), HPA assay kit (Product code: K-CERA, Megazyme brand), α-glucosidase assay kit (Product code: MAK123, Sigma aldrich), DPP-IV inhibitor screening assay kit (Product code: ab133081 Abcam), starch (Sigma aldrich) and all the other materials of analytical grade were used.

Techniques:

Journal: Dose-Response

Article Title: Biochemical Investigation of Inhibitory Activities of Plant-Derived Bioactive Compounds Against Carbohydrate and Glucagon-Like Peptide-1 Metabolizing Enzymes

doi: 10.1177/15593258221093275

Figure Lengend Snippet: Inhibitory Activity of Bioactive Compounds Against GLU, HPA, and DPP-IV Enzymes.

Article Snippet: Resveratrol (CHEM-IMPEX INT’L INC), taxifolin (Sigma aldrich), acarbose (Carbosnyth,USA), diprotin (Sigma aldrich), HPA assay kit (Product code: K-CERA, Megazyme brand), α-glucosidase assay kit (Product code: MAK123, Sigma aldrich), DPP-IV inhibitor screening assay kit (Product code: ab133081 Abcam), starch (Sigma aldrich) and all the other materials of analytical grade were used.

Techniques: Activity Assay, Positive Control

Journal: Dose-Response

Article Title: Biochemical Investigation of Inhibitory Activities of Plant-Derived Bioactive Compounds Against Carbohydrate and Glucagon-Like Peptide-1 Metabolizing Enzymes

doi: 10.1177/15593258221093275

Figure Lengend Snippet: Surflex Score of Docked Ligands; Taxifolin and Resveratrol for GLU, HPA, and DPP-IV Along With Their Corresponding Standard Molecules Acarbose, Miglitol, and Diprotin A.

Article Snippet: Resveratrol (CHEM-IMPEX INT’L INC), taxifolin (Sigma aldrich), acarbose (Carbosnyth,USA), diprotin (Sigma aldrich), HPA assay kit (Product code: K-CERA, Megazyme brand), α-glucosidase assay kit (Product code: MAK123, Sigma aldrich), DPP-IV inhibitor screening assay kit (Product code: ab133081 Abcam), starch (Sigma aldrich) and all the other materials of analytical grade were used.

Techniques:

Journal: American Journal of Physiology - Regulatory, Integrative and Comparative Physiology

Article Title: Greater natriuretic response to ENaC inhibition in male versus female Sprague-Dawley rats

doi: 10.1152/ajpregu.00060.2019

Figure Lengend Snippet: Antibodies used in immunoblotting

Article Snippet: β-ENaC , ProSci (Poway, CA) ( 21 , 34 ) , Rabbit , 1:2,000 , Invitrogen (SA5-10036) , Goat , 1:1,000.

Techniques:

Journal: American Journal of Physiology - Regulatory, Integrative and Comparative Physiology

Article Title: Greater natriuretic response to ENaC inhibition in male versus female Sprague-Dawley rats

doi: 10.1152/ajpregu.00060.2019

Figure Lengend Snippet: Immunoblots of kidney cortical tissue homogenates demonstrating abundance of α-, β-, and γ-ENaC (epithelial sodium channel) subunits in both male and female Sprague-Dawley rats. Tissues collected at either Zeitgeber time (ZT) 0 or ZT12. Representative blots of actin and Coomassie are demonstrated. Densitometry signals were normalized to male rats (set to 1.0) and expressed as means ± SE. *P < 0.05 vs. male group, unpaired t-test; n = 5/group.

Article Snippet: β-ENaC , ProSci (Poway, CA) ( 21 , 34 ) , Rabbit , 1:2,000 , Invitrogen (SA5-10036) , Goat , 1:1,000.

Techniques: Western Blot

Journal: Acta Biochimica et Biophysica Sinica

Article Title: Nanchangmycin suppresses influenza A virus infection by blocking endosomal acidification

doi: 10.3724/abbs.2025102

Figure Lengend Snippet: Screening of anti-influenza A virus (A) Schematic of antiviral screening by HA assay. MDCK cells were infected with H1N1 or mock-infected at an MOI of 0.001 in the presence of inhibitors from compound libraries at 37°C for 48 h, after which the cell supernatant was collected to test the viral titer via HA assay. Oseltamivir and DMSO were added to the cells as positive controls and negative controls, respectively. (B) The results of antiviral inhibitor screening. A total of 5545 compounds were tested in primary and secondary screens via HA assay and cytopathic effect assay, and 2 compounds that have not been reported for further studies on the basis of their potency were selected. (C) Chemical structures of zafirlukast and nanchangmycin. (D) Cytotoxicity of zafirlukast and nanchangmycin. Zafirlukast and nanchangmycin at the indicated concentrations in DMSO (0.1 μM‒1000 μM) were added to the MDCK cells. Cell viability was determined by CCK-8 assay. (E) Antiviral activity of zafirlukast and nanchangmycin at different concentrations without cytotoxicity. The experiments were performed in triplicate. The mean value is shown with the standard deviation (SD). *P < 0.05, **P < 0.01, ***P < 0.001.

Article Snippet: Oseltamivir carboxylate (OSV), oseltamivir phosphate (OSE), epigallocatechin gallate (EGCG) and ribavirin (RBV) were purchased from TargetMol.

Techniques: Virus, Hemagglutination Assay, Infection, CCK-8 Assay, Activity Assay, Standard Deviation

Journal: Acta Biochimica et Biophysica Sinica

Article Title: Nanchangmycin suppresses influenza A virus infection by blocking endosomal acidification

doi: 10.3724/abbs.2025102

Figure Lengend Snippet: Effects of nanchangmycin on influenza A virus infection in vivo (A) Schematic representation of the antiviral study in a mouse model. The mice were intranasally infected with H1N1 or PBS. At 1 d.p.i., the mice were intragastrically administered with nanchangmycin (1 mg/kg/day) or oseltamivir (10 mg/kg/day). The same treatment was administered once per day for 7 days. The mice were monitored daily for survival for 21 days. Mice that lost more than 25% of their initial body weight were sacrificed. Weight loss (B) and mortality (C) were monitored for 21 days. Nanchangmycin and OSE reduced the lung index (D) and viral load (E) in the lungs of infected mice compared with those of the negative control. The data are shown as the mean ± SD (n = 8 mice in each group). *P < 0.05, **P < 0.01, ***P < 0.001.

Article Snippet: Oseltamivir carboxylate (OSV), oseltamivir phosphate (OSE), epigallocatechin gallate (EGCG) and ribavirin (RBV) were purchased from TargetMol.

Techniques: Virus, Infection, In Vivo, Negative Control

Journal: Acta Biochimica et Biophysica Sinica

Article Title: Nanchangmycin suppresses influenza A virus infection by blocking endosomal acidification

doi: 10.3724/abbs.2025102

Figure Lengend Snippet: Effects of nanchangmycin on oseltamivir-resistant influenza virus infection in vitro MDCK cells were infected with oseltamivir-resistant influenza A virus at an MOI of 1 in the presence of nanchangmycin (0.1, 0.5 or 1 μM), bafilomycin (50 nM), oseltamivir (1 μM) and DMSO. Total RNA was extracted from cell lysates at 4, 12 or 24 h.i.p. The relative expression levels of viral mRNAs were assessed via RT-qPCR and normalized to that of β-actin mRNA. The data are presented as the mean ± SD.

Article Snippet: Oseltamivir carboxylate (OSV), oseltamivir phosphate (OSE), epigallocatechin gallate (EGCG) and ribavirin (RBV) were purchased from TargetMol.

Techniques: Virus, Infection, In Vitro, Expressing, Quantitative RT-PCR