40893 Search Results


h3l  (Sino Biological)
94
Sino Biological h3l
Graphical summary of anti-MPXV VHHs screening. Phage library presenting 3 × 10 9 VHHs was incubated with each biotin labeled-MPXV antigen (either A29L, A35R, B6R, E8L, <t>H3L,</t> or M1R), followed by washing unbound phages. After 3–4 rounds of phage display panning, antigen-bound phages were obtained. Binding of the obtained phage clones to the target antigen was validated by phage ELISA. VHH sequences were identified from the phagemids extracted from the validated clones, and monoclonal VHHs were expressed in periplasm fractions of E. coli BL21.
H3l, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/h3l/product/Sino Biological
Average 94 stars, based on 1 article reviews
h3l - by Bioz Stars, 2026-02
94/100 stars
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type strain dsm 40484 t  (DSMZ)
95
DSMZ type strain dsm 40484 t
Graphical summary of anti-MPXV VHHs screening. Phage library presenting 3 × 10 9 VHHs was incubated with each biotin labeled-MPXV antigen (either A29L, A35R, B6R, E8L, <t>H3L,</t> or M1R), followed by washing unbound phages. After 3–4 rounds of phage display panning, antigen-bound phages were obtained. Binding of the obtained phage clones to the target antigen was validated by phage ELISA. VHH sequences were identified from the phagemids extracted from the validated clones, and monoclonal VHHs were expressed in periplasm fractions of E. coli BL21.
Type Strain Dsm 40484 T, supplied by DSMZ, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/type strain dsm 40484 t/product/DSMZ
Average 95 stars, based on 1 article reviews
type strain dsm 40484 t - by Bioz Stars, 2026-02
95/100 stars
  Buy from Supplier

Image Search Results


Graphical summary of anti-MPXV VHHs screening. Phage library presenting 3 × 10 9 VHHs was incubated with each biotin labeled-MPXV antigen (either A29L, A35R, B6R, E8L, H3L, or M1R), followed by washing unbound phages. After 3–4 rounds of phage display panning, antigen-bound phages were obtained. Binding of the obtained phage clones to the target antigen was validated by phage ELISA. VHH sequences were identified from the phagemids extracted from the validated clones, and monoclonal VHHs were expressed in periplasm fractions of E. coli BL21.

Journal: Communications Biology

Article Title: Bivalent single-domain antibodies show potent mpox virus neutralization through M1R antigen

doi: 10.1038/s42003-025-08494-x

Figure Lengend Snippet: Graphical summary of anti-MPXV VHHs screening. Phage library presenting 3 × 10 9 VHHs was incubated with each biotin labeled-MPXV antigen (either A29L, A35R, B6R, E8L, H3L, or M1R), followed by washing unbound phages. After 3–4 rounds of phage display panning, antigen-bound phages were obtained. Binding of the obtained phage clones to the target antigen was validated by phage ELISA. VHH sequences were identified from the phagemids extracted from the validated clones, and monoclonal VHHs were expressed in periplasm fractions of E. coli BL21.

Article Snippet: Recombinant MPXV antigen proteins A29L (Ser21-Glu110 of QNP13733.1 in E.coli , Sino Biological 40891-V08E) , A35R (Arg58-Thr181 of EPI_ISL_13052264 in HEK293, Sino Biological 40886-V08H) , , B6R (Tyr18-His279 of QNP13760.1 in HEK293, Sino Biological 40902-V08H) , E8L (Met1-Thr275 of Q8V4Y0 in HEK293, RayBiotech 230-30232) , H3L (Met1-Pro278 of QNP13688.1 in HEK293, Sino Biological 40893-V08H1) , and M1R (Ala3-Gly183 of QNP13675.1 in HEK293, Sino Biological 40904-V07H) were used as target antigens.

Techniques: Incubation, Labeling, Binding Assay, Clone Assay, Enzyme-linked Immunosorbent Assay