Journal: NPJ Vaccines

Article Title: Immunization-induced antigen archiving enhances local memory CD8+ T cell responses following an unrelated viral infection

doi: 10.1038/s41541-024-00856-6

Figure Lengend Snippet: a Experimental schematic for b – e . C57/BL6 mice were immunized subcutaneously in the footpad and/or flank with the indicated antigens and adjuvants. b Cells were stained with CD45, PDPN, CD31 and PD-L1. Cells were gated on CD45-PDPN + CD31- for FRC and CD45-PDPN + CD31+ for LECs. Shown are examples of LEC and FRC antigen-positive cells based on PD-L1 expression (floor, MARCO LEC) and ova-AF488+ from mice 2–3 weeks after immunization with ova conjugated to Alexa-Fluor 488 (AF488) and polyI:C and αCD40. c Quantification of the frequency of LEC, BEC, and FRC in the popliteal lymph node (pLN) that are positive for the indicated antigens administered with polyI:C and αCD40 at indicated time. d Same as ( b ) except for mice were immunized with SARS-CoV-2-RBD-AF488, polyI:C, and αCD40. e Same as in ( c ) except for SARS-CoV-2-RBD and CHIKV-E2 with polyI:C and αCD40. CHIKV-E2 was repeated for 9–14 days post-vaccine (~2 weeks). Statistical analysis was done using an unpaired t -test where the p -value between naïve and indicated antigen is <0.0001. In each experiment, at least n = 2–3 mice per group were evaluated and the experiment was repeated n = 2–5 times for c – e . Shown is the representative data from one of the experiments. Error bars are mean ± standard error of the mean. ns not significant, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: Ova (10 μg) was purchased from Sigma-Aldrich (Cat No. A5503) and Chikungunya virus envelope 2 protein (8 μg) (CHIKV-E2, strain SL-CK1) was purchased from Sino Biological (Cat. No. 40440-V08B).

Techniques: Staining, Expressing

Journal: Applied Microbiology and Biotechnology

Article Title: A broadly applicable split-luciferase biosensor approach for rapid antibody detection in emerging infectious diseases

doi: 10.1007/s00253-026-13712-5

Figure Lengend Snippet: Sensor pair selection for detection of SARS-CoV-2 and CHIKV antibodies. a Different sensor combinations were screened for their ability to reconstitute luciferase activity in the presence of anti-SARS-CoV-2 RBD antibodies. b Sensor pairs were similarly evaluated for luciferase complementation in the presence of anti-CHIKV E2 antibodies. Data represent means of duplicate tests; error bars indicate standard deviations

Article Snippet: The affinity-purified rabbit polyclonal antibodies against SARS-CoV-2 Spike RBD (K110843P, Solarbio, China) or against CHIKV E2 (40,440-T46; Sino Biological, China) (0.1 μL) were mixed with 40 ng of the LgBiT/C2 and SmBiT/C2 fusion sensors in dilution buffer (PBST containing 1% BSA); then, 100 μL of the mixture was added to plates and incubated for 30 min at 37 °C.

Techniques: Selection, Luciferase, Activity Assay

Journal: Applied Microbiology and Biotechnology

Article Title: A broadly applicable split-luciferase biosensor approach for rapid antibody detection in emerging infectious diseases

doi: 10.1007/s00253-026-13712-5

Figure Lengend Snippet: Analytical performance of the bioluminescent immunoassay for detection of anti-SARS-CoV-2 RBD (6.39 mg/mL) and anti-CHIKV E2 antibodies (1.0 mg/mL). Data represent means of duplicate tests; error bars indicate standard deviations

Article Snippet: The affinity-purified rabbit polyclonal antibodies against SARS-CoV-2 Spike RBD (K110843P, Solarbio, China) or against CHIKV E2 (40,440-T46; Sino Biological, China) (0.1 μL) were mixed with 40 ng of the LgBiT/C2 and SmBiT/C2 fusion sensors in dilution buffer (PBST containing 1% BSA); then, 100 μL of the mixture was added to plates and incubated for 30 min at 37 °C.

Techniques: