Journal: Scientific Reports
Article Title: Cross-reactivity between avian influenza A (H7N9) virus and divergent H7 subtypic- and heterosubtypic influenza A viruses
Figure Lengend Snippet: Cross-reactivity between H7N9 HA and antibodies against heterosubtypes of influenza A viruses. ( A,B ) Cross-reactivities in ELISA. ELISA tests were performed with H7N9 HA expressed in insect cells as coating antigen. Antisera against H1, H2, H3, H4, H5, and H8 ( A ), and H9, H10, H11, H12, H13, and H16 ( B ) were serially diluted starting at a dilution of 256 ng/ml to react with the coating antigens. Antisera against H7N9 HA were used as positive control. ( C ) Cross-reactivities in indirect immunofluorescence assays. MDCK cells infected with Anhui/1 at an MOI of 0.1 were fixed with 4% formaldehyde and probed with antisera against HA proteins of H1, H2, H3, H4, H5, H8, H9, H10, H11, H12, H13, and H16 with a concentration of 0.5 μg/ml. Antisera against H7N9 HA were used as positive control. ( D ) Cross-reactivities in Hemagglutination inhibition (HI) assays. The assays were carried out using the A/Anhui/1/2013 (H7N9) strain and antisera against whole virus of H1N1, H3N2, and H5N1 with antisera against H7N9-, H7N2-, H7N3-, and H7N7-HA as positive controls. Serum with titers > 40 were considered HI-positive for H7N9 virus.
Article Snippet: The antibodies against HA proteins of H1, H2, H3, H4, H5, H7N9, H8, H9, H10, H11, H12, H13, and H16 were produced in rabbits immunized with the corresponding purified recombinant HA proteins and the antibodies were purified by Protein A affinity chromatography (Sino Biological).
Techniques: Enzyme-linked Immunosorbent Assay, Positive Control, Immunofluorescence, Infection, Concentration Assay, HI Assay