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    Sino Biological h10
    Cross-reactivity between H7N9 HA and antibodies against heterosubtypes of influenza A viruses. ( A,B ) Cross-reactivities in ELISA. ELISA tests were performed with H7N9 HA expressed in insect cells as coating antigen. Antisera against H1, H2, H3, H4, H5, and H8 ( A ), and H9, <t>H10,</t> H11, H12, H13, and H16 ( B ) were serially diluted starting at a dilution of 256 ng/ml to react with the coating antigens. Antisera against H7N9 HA were used as positive control. ( C ) Cross-reactivities in indirect immunofluorescence assays. MDCK cells infected with Anhui/1 at an MOI of 0.1 were fixed with 4% formaldehyde and probed with antisera against HA proteins of H1, H2, H3, H4, H5, H8, H9, H10, H11, H12, H13, and H16 with a concentration of 0.5 μg/ml. Antisera against H7N9 HA were used as positive control. ( D ) Cross-reactivities in Hemagglutination inhibition (HI) assays. The assays were carried out using the A/Anhui/1/2013 (H7N9) strain and antisera against whole virus of H1N1, H3N2, and H5N1 with antisera against H7N9-, H7N2-, H7N3-, and H7N7-HA as positive controls. Serum with titers > 40 were considered HI-positive for H7N9 virus.
    H10, supplied by Sino Biological, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cross-reactivity between H7N9 HA and antibodies against heterosubtypes of influenza A viruses. ( A,B ) Cross-reactivities in ELISA. ELISA tests were performed with H7N9 HA expressed in insect cells as coating antigen. Antisera against H1, H2, H3, H4, H5, and H8 ( A ), and H9, H10, H11, H12, H13, and H16 ( B ) were serially diluted starting at a dilution of 256 ng/ml to react with the coating antigens. Antisera against H7N9 HA were used as positive control. ( C ) Cross-reactivities in indirect immunofluorescence assays. MDCK cells infected with Anhui/1 at an MOI of 0.1 were fixed with 4% formaldehyde and probed with antisera against HA proteins of H1, H2, H3, H4, H5, H8, H9, H10, H11, H12, H13, and H16 with a concentration of 0.5 μg/ml. Antisera against H7N9 HA were used as positive control. ( D ) Cross-reactivities in Hemagglutination inhibition (HI) assays. The assays were carried out using the A/Anhui/1/2013 (H7N9) strain and antisera against whole virus of H1N1, H3N2, and H5N1 with antisera against H7N9-, H7N2-, H7N3-, and H7N7-HA as positive controls. Serum with titers > 40 were considered HI-positive for H7N9 virus.

    Journal: Scientific Reports

    Article Title: Cross-reactivity between avian influenza A (H7N9) virus and divergent H7 subtypic- and heterosubtypic influenza A viruses

    doi: 10.1038/srep22045

    Figure Lengend Snippet: Cross-reactivity between H7N9 HA and antibodies against heterosubtypes of influenza A viruses. ( A,B ) Cross-reactivities in ELISA. ELISA tests were performed with H7N9 HA expressed in insect cells as coating antigen. Antisera against H1, H2, H3, H4, H5, and H8 ( A ), and H9, H10, H11, H12, H13, and H16 ( B ) were serially diluted starting at a dilution of 256 ng/ml to react with the coating antigens. Antisera against H7N9 HA were used as positive control. ( C ) Cross-reactivities in indirect immunofluorescence assays. MDCK cells infected with Anhui/1 at an MOI of 0.1 were fixed with 4% formaldehyde and probed with antisera against HA proteins of H1, H2, H3, H4, H5, H8, H9, H10, H11, H12, H13, and H16 with a concentration of 0.5 μg/ml. Antisera against H7N9 HA were used as positive control. ( D ) Cross-reactivities in Hemagglutination inhibition (HI) assays. The assays were carried out using the A/Anhui/1/2013 (H7N9) strain and antisera against whole virus of H1N1, H3N2, and H5N1 with antisera against H7N9-, H7N2-, H7N3-, and H7N7-HA as positive controls. Serum with titers > 40 were considered HI-positive for H7N9 virus.

    Article Snippet: The antibodies against HA proteins of H1, H2, H3, H4, H5, H7N9, H8, H9, H10, H11, H12, H13, and H16 were produced in rabbits immunized with the corresponding purified recombinant HA proteins and the antibodies were purified by Protein A affinity chromatography (Sino Biological).

    Techniques: Enzyme-linked Immunosorbent Assay, Positive Control, Immunofluorescence, Infection, Concentration Assay, HI Assay

    Cross-reactivities between the HA proteins of heterosubtypes of influenza A viruses and antibodies against H7N9. The cross-reactivities were analyzed using ELISA ( A,B ) and Western blot ( C ) with recombinant HA proteins of H1, H2, H3, H4, H5, H6, H8, H9, H10, H11, H12, H13, and H16 as antigens. HA proteins were two-fold diluted with a starting dilution of 2,560 ng/ml.

    Journal: Scientific Reports

    Article Title: Cross-reactivity between avian influenza A (H7N9) virus and divergent H7 subtypic- and heterosubtypic influenza A viruses

    doi: 10.1038/srep22045

    Figure Lengend Snippet: Cross-reactivities between the HA proteins of heterosubtypes of influenza A viruses and antibodies against H7N9. The cross-reactivities were analyzed using ELISA ( A,B ) and Western blot ( C ) with recombinant HA proteins of H1, H2, H3, H4, H5, H6, H8, H9, H10, H11, H12, H13, and H16 as antigens. HA proteins were two-fold diluted with a starting dilution of 2,560 ng/ml.

    Article Snippet: The antibodies against HA proteins of H1, H2, H3, H4, H5, H7N9, H8, H9, H10, H11, H12, H13, and H16 were produced in rabbits immunized with the corresponding purified recombinant HA proteins and the antibodies were purified by Protein A affinity chromatography (Sino Biological).

    Techniques: Enzyme-linked Immunosorbent Assay, Western Blot, Recombinant

    Identification of the cross-reactive regions by Western blot. The lysates of MDCK cells infected with Anhui/1 ( A ), Shanghai/1 ( B ), and Shanghai/2 ( C ) isolates at an MOI of 0.1 were harvested 48 h post infection and probed with antisera to HA proteins of H1, H2, H3, H4, H5, H8, H9, H10, H11, H12, H13, and H16. Antisera against H7N9 HA were used as positive control.

    Journal: Scientific Reports

    Article Title: Cross-reactivity between avian influenza A (H7N9) virus and divergent H7 subtypic- and heterosubtypic influenza A viruses

    doi: 10.1038/srep22045

    Figure Lengend Snippet: Identification of the cross-reactive regions by Western blot. The lysates of MDCK cells infected with Anhui/1 ( A ), Shanghai/1 ( B ), and Shanghai/2 ( C ) isolates at an MOI of 0.1 were harvested 48 h post infection and probed with antisera to HA proteins of H1, H2, H3, H4, H5, H8, H9, H10, H11, H12, H13, and H16. Antisera against H7N9 HA were used as positive control.

    Article Snippet: The antibodies against HA proteins of H1, H2, H3, H4, H5, H7N9, H8, H9, H10, H11, H12, H13, and H16 were produced in rabbits immunized with the corresponding purified recombinant HA proteins and the antibodies were purified by Protein A affinity chromatography (Sino Biological).

    Techniques: Western Blot, Infection, Positive Control