40225 Search Results


mouse tgfbr3  (Santa Cruz Biotechnology)
86
Santa Cruz Biotechnology mouse tgfbr3
FIGURE 3. Overexpression of <t>TGFBR3</t> drove the Acvrl1/Smad1 axis. A, the impact of the overexpression of human TGFBR3 in NIH/3T3 cells in Smad1/5/8 phosphorylation was assessed by immunoblot, employing pIRES::TGFBR3 for TGFBR3 overexpression or pIRES as empty vector. Identical data were obtained with the mouse Tgfbr3-expressing construct; however, the increased expression of human TGFBR3 over the background, endogenous mouse Tgfbr3 was more evident, and hence, these data are presented here. B, expression changes in Tgfbr3 in the TGF--stimulated groups were assessed by densitometry, where p values compare mean values in the pIRES-transfected versus pIRES::TGFBR3-transfected cells. C, to validate that the expression of TGFBR3 can (in the absence of TGF- stimulation) drive Acvrl1/Smad1 signaling, the expression of the Smad1-responsive “BMP-responsive element” in pBRE-luc was assessed by Dual- Luciferase assay, in the presence of either pIRES::TGFBR3 or pIRES as empty vector. The data represent means S.D. (n 6), and p values were assessed by unpaired Student’s t test.
Mouse Tgfbr3, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plenti4 flag p85 dkrmns560del  (Addgene inc)
93
Addgene inc plenti4 flag p85 dkrmns560del
FIGURE 3. Overexpression of <t>TGFBR3</t> drove the Acvrl1/Smad1 axis. A, the impact of the overexpression of human TGFBR3 in NIH/3T3 cells in Smad1/5/8 phosphorylation was assessed by immunoblot, employing pIRES::TGFBR3 for TGFBR3 overexpression or pIRES as empty vector. Identical data were obtained with the mouse Tgfbr3-expressing construct; however, the increased expression of human TGFBR3 over the background, endogenous mouse Tgfbr3 was more evident, and hence, these data are presented here. B, expression changes in Tgfbr3 in the TGF--stimulated groups were assessed by densitometry, where p values compare mean values in the pIRES-transfected versus pIRES::TGFBR3-transfected cells. C, to validate that the expression of TGFBR3 can (in the absence of TGF- stimulation) drive Acvrl1/Smad1 signaling, the expression of the Smad1-responsive “BMP-responsive element” in pBRE-luc was assessed by Dual- Luciferase assay, in the presence of either pIRES::TGFBR3 or pIRES as empty vector. The data represent means S.D. (n 6), and p values were assessed by unpaired Student’s t test.
Plenti4 Flag P85 Dkrmns560del, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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moorenstrasse 5, 40225 düsseldorf  (Schauer Agrotronic)
90
Schauer Agrotronic moorenstrasse 5, 40225 düsseldorf
FIGURE 3. Overexpression of <t>TGFBR3</t> drove the Acvrl1/Smad1 axis. A, the impact of the overexpression of human TGFBR3 in NIH/3T3 cells in Smad1/5/8 phosphorylation was assessed by immunoblot, employing pIRES::TGFBR3 for TGFBR3 overexpression or pIRES as empty vector. Identical data were obtained with the mouse Tgfbr3-expressing construct; however, the increased expression of human TGFBR3 over the background, endogenous mouse Tgfbr3 was more evident, and hence, these data are presented here. B, expression changes in Tgfbr3 in the TGF--stimulated groups were assessed by densitometry, where p values compare mean values in the pIRES-transfected versus pIRES::TGFBR3-transfected cells. C, to validate that the expression of TGFBR3 can (in the absence of TGF- stimulation) drive Acvrl1/Smad1 signaling, the expression of the Smad1-responsive “BMP-responsive element” in pBRE-luc was assessed by Dual- Luciferase assay, in the presence of either pIRES::TGFBR3 or pIRES as empty vector. The data represent means S.D. (n 6), and p values were assessed by unpaired Student’s t test.
Moorenstrasse 5, 40225 Düsseldorf, supplied by Schauer Agrotronic, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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his tag  (BPS Bioscience)
91
BPS Bioscience his tag
FIGURE 3. Overexpression of <t>TGFBR3</t> drove the Acvrl1/Smad1 axis. A, the impact of the overexpression of human TGFBR3 in NIH/3T3 cells in Smad1/5/8 phosphorylation was assessed by immunoblot, employing pIRES::TGFBR3 for TGFBR3 overexpression or pIRES as empty vector. Identical data were obtained with the mouse Tgfbr3-expressing construct; however, the increased expression of human TGFBR3 over the background, endogenous mouse Tgfbr3 was more evident, and hence, these data are presented here. B, expression changes in Tgfbr3 in the TGF--stimulated groups were assessed by densitometry, where p values compare mean values in the pIRES-transfected versus pIRES::TGFBR3-transfected cells. C, to validate that the expression of TGFBR3 can (in the absence of TGF- stimulation) drive Acvrl1/Smad1 signaling, the expression of the Smad1-responsive “BMP-responsive element” in pBRE-luc was assessed by Dual- Luciferase assay, in the presence of either pIRES::TGFBR3 or pIRES as empty vector. The data represent means S.D. (n 6), and p values were assessed by unpaired Student’s t test.
His Tag, supplied by BPS Bioscience, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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FIGURE 3. Overexpression of TGFBR3 drove the Acvrl1/Smad1 axis. A, the impact of the overexpression of human TGFBR3 in NIH/3T3 cells in Smad1/5/8 phosphorylation was assessed by immunoblot, employing pIRES::TGFBR3 for TGFBR3 overexpression or pIRES as empty vector. Identical data were obtained with the mouse Tgfbr3-expressing construct; however, the increased expression of human TGFBR3 over the background, endogenous mouse Tgfbr3 was more evident, and hence, these data are presented here. B, expression changes in Tgfbr3 in the TGF--stimulated groups were assessed by densitometry, where p values compare mean values in the pIRES-transfected versus pIRES::TGFBR3-transfected cells. C, to validate that the expression of TGFBR3 can (in the absence of TGF- stimulation) drive Acvrl1/Smad1 signaling, the expression of the Smad1-responsive “BMP-responsive element” in pBRE-luc was assessed by Dual- Luciferase assay, in the presence of either pIRES::TGFBR3 or pIRES as empty vector. The data represent means S.D. (n 6), and p values were assessed by unpaired Student’s t test.

Journal: Journal of Biological Chemistry

Article Title: Glucocorticoids Recruit Tgfbr3 and Smad1 to Shift Transforming Growth Factor-β Signaling from the Tgfbr1/Smad2/3 Axis to the Acvrl1/Smad1 Axis in Lung Fibroblasts

doi: 10.1074/jbc.m113.541052

Figure Lengend Snippet: FIGURE 3. Overexpression of TGFBR3 drove the Acvrl1/Smad1 axis. A, the impact of the overexpression of human TGFBR3 in NIH/3T3 cells in Smad1/5/8 phosphorylation was assessed by immunoblot, employing pIRES::TGFBR3 for TGFBR3 overexpression or pIRES as empty vector. Identical data were obtained with the mouse Tgfbr3-expressing construct; however, the increased expression of human TGFBR3 over the background, endogenous mouse Tgfbr3 was more evident, and hence, these data are presented here. B, expression changes in Tgfbr3 in the TGF--stimulated groups were assessed by densitometry, where p values compare mean values in the pIRES-transfected versus pIRES::TGFBR3-transfected cells. C, to validate that the expression of TGFBR3 can (in the absence of TGF- stimulation) drive Acvrl1/Smad1 signaling, the expression of the Smad1-responsive “BMP-responsive element” in pBRE-luc was assessed by Dual- Luciferase assay, in the presence of either pIRES::TGFBR3 or pIRES as empty vector. The data represent means S.D. (n 6), and p values were assessed by unpaired Student’s t test.

Article Snippet: The siRNA were from Santa Cruz, and the optimal working concentration was assessed for each siRNA, as: mouse Tgfbr3 (sc-40225; 200 nM) and mouse FEBRUARY 7, 2014 • VOLUME 289 • NUMBER 6 JOURNAL OF BIOLOGICAL CHEMISTRY 3263 at C arleton U niv - O C U L on June 16, 2014 http://w w w .jbc.org/ D ow nloaded from Smad1 (sc-36507; 200 nM).

Techniques: Over Expression, Phospho-proteomics, Western Blot, Plasmid Preparation, Expressing, Construct, Transfection, Luciferase

FIGURE 7. Glucocorticoids modulate expression of the TGF- signaling machinery in the lungs of living mice. To assess whether the effects observed in NIH/3T3 cells and primary human lung fibroblasts were applicable in vivo in living mice, six adult female C57Bl/6J mice received an intraperitoneal injection of dexamethasone (10 mg/kg body mass), whereas six control adult female C57Bl/6J mice received an intraperitoneal injection of vehicle alone (PBS, 200 l). 24 h later, mouse tissues were harvested for analysis. A, assessment of tgfbr3, acvrl1, and smad1 expression by real time RT-PCR in the lungs, heart, kidney, and liver of dexamethasone (dex)- and vehicle-treated mice. The data represent means S.D. (n 6), and p values were assessed by unpaired Student’s t test and compare gene expression in dexamethasone-treated versus vehicle-treated mice. B, the expression of Tgfbr3 and total and phosphorylated Smad1 and Smad2 were assessed by immunoblot in mouse lung tissues. C, data were quantified by densitometric analysis, where data represent mean S.D. (n 5/group), and p values were assessed by unpaired Student’s t test.

Journal: Journal of Biological Chemistry

Article Title: Glucocorticoids Recruit Tgfbr3 and Smad1 to Shift Transforming Growth Factor-β Signaling from the Tgfbr1/Smad2/3 Axis to the Acvrl1/Smad1 Axis in Lung Fibroblasts

doi: 10.1074/jbc.m113.541052

Figure Lengend Snippet: FIGURE 7. Glucocorticoids modulate expression of the TGF- signaling machinery in the lungs of living mice. To assess whether the effects observed in NIH/3T3 cells and primary human lung fibroblasts were applicable in vivo in living mice, six adult female C57Bl/6J mice received an intraperitoneal injection of dexamethasone (10 mg/kg body mass), whereas six control adult female C57Bl/6J mice received an intraperitoneal injection of vehicle alone (PBS, 200 l). 24 h later, mouse tissues were harvested for analysis. A, assessment of tgfbr3, acvrl1, and smad1 expression by real time RT-PCR in the lungs, heart, kidney, and liver of dexamethasone (dex)- and vehicle-treated mice. The data represent means S.D. (n 6), and p values were assessed by unpaired Student’s t test and compare gene expression in dexamethasone-treated versus vehicle-treated mice. B, the expression of Tgfbr3 and total and phosphorylated Smad1 and Smad2 were assessed by immunoblot in mouse lung tissues. C, data were quantified by densitometric analysis, where data represent mean S.D. (n 5/group), and p values were assessed by unpaired Student’s t test.

Article Snippet: The siRNA were from Santa Cruz, and the optimal working concentration was assessed for each siRNA, as: mouse Tgfbr3 (sc-40225; 200 nM) and mouse FEBRUARY 7, 2014 • VOLUME 289 • NUMBER 6 JOURNAL OF BIOLOGICAL CHEMISTRY 3263 at C arleton U niv - O C U L on June 16, 2014 http://w w w .jbc.org/ D ow nloaded from Smad1 (sc-36507; 200 nM).

Techniques: Expressing, In Vivo, Injection, Control, Quantitative RT-PCR, Gene Expression, Western Blot