4 parameter logistic model Search Results


curves generated from raw fluorescence data using a 4-parameter variable-slope hill model in graphpad prism 7.0  (GraphPad Software Inc)
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Ethoxylated Surfactants May Promote Adipogenesis via Inhibition of TRβ. Antagonist activity for the TRβ as measured via FRET reporter gene assay using the TRβ GeneBLAzer Reporter Assay as described in the Methods. Percent agonist TRβ activity for the T3 positive control relative to its maximum response (A), percent TRβ antagonism of intra-assay EC80 T3 (concentration that exhibits 80% of maximum T3 response; 0.3 nM) for various alkyl chain length ethoxylated surfactants (B), inhibition or enhancement of cell viability relative to vehicle control for various alkyl chain length ethoxylated surfactants (C), percent TRβ antagonism relative to intra-assay EC80 T3 for various ethoxylate chain length NPEOs (D), and inhibition or enhancement of cell viability relative to vehicle control for various ethoxylate chain length NPEOs (E). Data presented as mean ± SEM from 3 independent experiments. * indicates lowest concentration with significant antagonist activity relative to EC80 T3, p < .05, as per 1-way ANOVA and Dunnett’s posthoc test in GraphPad Prism 7.0.
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Ethoxylated Surfactants May Promote Adipogenesis via Inhibition of TRβ. Antagonist activity for the TRβ as measured via FRET reporter gene assay using the TRβ GeneBLAzer Reporter Assay as described in the Methods. Percent agonist TRβ activity for the T3 positive control relative to its maximum response (A), percent TRβ antagonism of intra-assay EC80 T3 (concentration that exhibits 80% of maximum T3 response; 0.3 nM) for various alkyl chain length ethoxylated surfactants (B), inhibition or enhancement of cell viability relative to vehicle control for various alkyl chain length ethoxylated surfactants (C), percent TRβ antagonism relative to intra-assay EC80 T3 for various ethoxylate chain length NPEOs (D), and inhibition or enhancement of cell viability relative to vehicle control for various ethoxylate chain length NPEOs (E). Data presented as mean ± SEM from 3 independent experiments. * indicates lowest concentration with significant antagonist activity relative to EC80 T3, p < .05, as per 1-way ANOVA and Dunnett’s posthoc test in GraphPad Prism 7.0.
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Ethoxylated Surfactants May Promote Adipogenesis via Inhibition of TRβ. Antagonist activity for the TRβ as measured via FRET reporter gene assay using the TRβ GeneBLAzer Reporter Assay as described in the Methods. Percent agonist TRβ activity for the T3 positive control relative to its maximum response (A), percent TRβ antagonism of intra-assay EC80 T3 (concentration that exhibits 80% of maximum T3 response; 0.3 nM) for various alkyl chain length ethoxylated surfactants (B), inhibition or enhancement of cell viability relative to vehicle control for various alkyl chain length ethoxylated surfactants (C), percent TRβ antagonism relative to intra-assay EC80 T3 for various ethoxylate chain length NPEOs (D), and inhibition or enhancement of cell viability relative to vehicle control for various ethoxylate chain length NPEOs (E). Data presented as mean ± SEM from 3 independent experiments. * indicates lowest concentration with significant antagonist activity relative to EC80 T3, p < .05, as per 1-way ANOVA and Dunnett’s posthoc test in GraphPad Prism 7.0.
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sigmoidal four-parameter logistic model implemented in graphpad prism 9.4.1  (GraphPad Software Inc)
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Ethoxylated Surfactants May Promote Adipogenesis via Inhibition of TRβ. Antagonist activity for the TRβ as measured via FRET reporter gene assay using the TRβ GeneBLAzer Reporter Assay as described in the Methods. Percent agonist TRβ activity for the T3 positive control relative to its maximum response (A), percent TRβ antagonism of intra-assay EC80 T3 (concentration that exhibits 80% of maximum T3 response; 0.3 nM) for various alkyl chain length ethoxylated surfactants (B), inhibition or enhancement of cell viability relative to vehicle control for various alkyl chain length ethoxylated surfactants (C), percent TRβ antagonism relative to intra-assay EC80 T3 for various ethoxylate chain length NPEOs (D), and inhibition or enhancement of cell viability relative to vehicle control for various ethoxylate chain length NPEOs (E). Data presented as mean ± SEM from 3 independent experiments. * indicates lowest concentration with significant antagonist activity relative to EC80 T3, p < .05, as per 1-way ANOVA and Dunnett’s posthoc test in GraphPad Prism 7.0.
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Ethoxylated Surfactants May Promote Adipogenesis via Inhibition of TRβ. Antagonist activity for the TRβ as measured via FRET reporter gene assay using the TRβ GeneBLAzer Reporter Assay as described in the Methods. Percent agonist TRβ activity for the T3 positive control relative to its maximum response (A), percent TRβ antagonism of intra-assay EC80 T3 (concentration that exhibits 80% of maximum T3 response; 0.3 nM) for various alkyl chain length ethoxylated surfactants (B), inhibition or enhancement of cell viability relative to vehicle control for various alkyl chain length ethoxylated surfactants (C), percent TRβ antagonism relative to intra-assay EC80 T3 for various ethoxylate chain length NPEOs (D), and inhibition or enhancement of cell viability relative to vehicle control for various ethoxylate chain length NPEOs (E). Data presented as mean ± SEM from 3 independent experiments. * indicates lowest concentration with significant antagonist activity relative to EC80 T3, p < .05, as per 1-way ANOVA and Dunnett’s posthoc test in GraphPad Prism 7.0.
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sigmoidal, four-parameter logistic curve model of tecan magellantm software v7.2  (Tecan Systems)
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Ethoxylated Surfactants May Promote Adipogenesis via Inhibition of TRβ. Antagonist activity for the TRβ as measured via FRET reporter gene assay using the TRβ GeneBLAzer Reporter Assay as described in the Methods. Percent agonist TRβ activity for the T3 positive control relative to its maximum response (A), percent TRβ antagonism of intra-assay EC80 T3 (concentration that exhibits 80% of maximum T3 response; 0.3 nM) for various alkyl chain length ethoxylated surfactants (B), inhibition or enhancement of cell viability relative to vehicle control for various alkyl chain length ethoxylated surfactants (C), percent TRβ antagonism relative to intra-assay EC80 T3 for various ethoxylate chain length NPEOs (D), and inhibition or enhancement of cell viability relative to vehicle control for various ethoxylate chain length NPEOs (E). Data presented as mean ± SEM from 3 independent experiments. * indicates lowest concentration with significant antagonist activity relative to EC80 T3, p < .05, as per 1-way ANOVA and Dunnett’s posthoc test in GraphPad Prism 7.0.
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4 parameter logistic nonlinear regression model  (ProMat Inc)
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Ethoxylated Surfactants May Promote Adipogenesis via Inhibition of TRβ. Antagonist activity for the TRβ as measured via FRET reporter gene assay using the TRβ GeneBLAzer Reporter Assay as described in the Methods. Percent agonist TRβ activity for the T3 positive control relative to its maximum response (A), percent TRβ antagonism of intra-assay EC80 T3 (concentration that exhibits 80% of maximum T3 response; 0.3 nM) for various alkyl chain length ethoxylated surfactants (B), inhibition or enhancement of cell viability relative to vehicle control for various alkyl chain length ethoxylated surfactants (C), percent TRβ antagonism relative to intra-assay EC80 T3 for various ethoxylate chain length NPEOs (D), and inhibition or enhancement of cell viability relative to vehicle control for various ethoxylate chain length NPEOs (E). Data presented as mean ± SEM from 3 independent experiments. * indicates lowest concentration with significant antagonist activity relative to EC80 T3, p < .05, as per 1-way ANOVA and Dunnett’s posthoc test in GraphPad Prism 7.0.
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nonlinear 4 parameter logistic (4pl) model  (OriginLab corp)
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Ethoxylated Surfactants May Promote Adipogenesis via Inhibition of TRβ. Antagonist activity for the TRβ as measured via FRET reporter gene assay using the TRβ GeneBLAzer Reporter Assay as described in the Methods. Percent agonist TRβ activity for the T3 positive control relative to its maximum response (A), percent TRβ antagonism of intra-assay EC80 T3 (concentration that exhibits 80% of maximum T3 response; 0.3 nM) for various alkyl chain length ethoxylated surfactants (B), inhibition or enhancement of cell viability relative to vehicle control for various alkyl chain length ethoxylated surfactants (C), percent TRβ antagonism relative to intra-assay EC80 T3 for various ethoxylate chain length NPEOs (D), and inhibition or enhancement of cell viability relative to vehicle control for various ethoxylate chain length NPEOs (E). Data presented as mean ± SEM from 3 independent experiments. * indicates lowest concentration with significant antagonist activity relative to EC80 T3, p < .05, as per 1-way ANOVA and Dunnett’s posthoc test in GraphPad Prism 7.0.
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four-parameter log-logistic model v. 5.02  (GraphPad Software Inc)
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Ethoxylated Surfactants May Promote Adipogenesis via Inhibition of TRβ. Antagonist activity for the TRβ as measured via FRET reporter gene assay using the TRβ GeneBLAzer Reporter Assay as described in the Methods. Percent agonist TRβ activity for the T3 positive control relative to its maximum response (A), percent TRβ antagonism of intra-assay EC80 T3 (concentration that exhibits 80% of maximum T3 response; 0.3 nM) for various alkyl chain length ethoxylated surfactants (B), inhibition or enhancement of cell viability relative to vehicle control for various alkyl chain length ethoxylated surfactants (C), percent TRβ antagonism relative to intra-assay EC80 T3 for various ethoxylate chain length NPEOs (D), and inhibition or enhancement of cell viability relative to vehicle control for various ethoxylate chain length NPEOs (E). Data presented as mean ± SEM from 3 independent experiments. * indicates lowest concentration with significant antagonist activity relative to EC80 T3, p < .05, as per 1-way ANOVA and Dunnett’s posthoc test in GraphPad Prism 7.0.
Four Parameter Log Logistic Model V. 5.02, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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four-parameter logistic curve fitting model bd cba software  (Becton Dickinson)
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Ethoxylated Surfactants May Promote Adipogenesis via Inhibition of TRβ. Antagonist activity for the TRβ as measured via FRET reporter gene assay using the TRβ GeneBLAzer Reporter Assay as described in the Methods. Percent agonist TRβ activity for the T3 positive control relative to its maximum response (A), percent TRβ antagonism of intra-assay EC80 T3 (concentration that exhibits 80% of maximum T3 response; 0.3 nM) for various alkyl chain length ethoxylated surfactants (B), inhibition or enhancement of cell viability relative to vehicle control for various alkyl chain length ethoxylated surfactants (C), percent TRβ antagonism relative to intra-assay EC80 T3 for various ethoxylate chain length NPEOs (D), and inhibition or enhancement of cell viability relative to vehicle control for various ethoxylate chain length NPEOs (E). Data presented as mean ± SEM from 3 independent experiments. * indicates lowest concentration with significant antagonist activity relative to EC80 T3, p < .05, as per 1-way ANOVA and Dunnett’s posthoc test in GraphPad Prism 7.0.
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curve-fitting of the four-parameter nonlinear-logistic-regression model graphpad prism  (GraphPad Software Inc)
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Ethoxylated Surfactants May Promote Adipogenesis via Inhibition of TRβ. Antagonist activity for the TRβ as measured via FRET reporter gene assay using the TRβ GeneBLAzer Reporter Assay as described in the Methods. Percent agonist TRβ activity for the T3 positive control relative to its maximum response (A), percent TRβ antagonism of intra-assay EC80 T3 (concentration that exhibits 80% of maximum T3 response; 0.3 nM) for various alkyl chain length ethoxylated surfactants (B), inhibition or enhancement of cell viability relative to vehicle control for various alkyl chain length ethoxylated surfactants (C), percent TRβ antagonism relative to intra-assay EC80 T3 for various ethoxylate chain length NPEOs (D), and inhibition or enhancement of cell viability relative to vehicle control for various ethoxylate chain length NPEOs (E). Data presented as mean ± SEM from 3 independent experiments. * indicates lowest concentration with significant antagonist activity relative to EC80 T3, p < .05, as per 1-way ANOVA and Dunnett’s posthoc test in GraphPad Prism 7.0.
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Ethoxylated Surfactants May Promote Adipogenesis via Inhibition of TRβ. Antagonist activity for the TRβ as measured via FRET reporter gene assay using the TRβ GeneBLAzer Reporter Assay as described in the Methods. Percent agonist TRβ activity for the T3 positive control relative to its maximum response (A), percent TRβ antagonism of intra-assay EC80 T3 (concentration that exhibits 80% of maximum T3 response; 0.3 nM) for various alkyl chain length ethoxylated surfactants (B), inhibition or enhancement of cell viability relative to vehicle control for various alkyl chain length ethoxylated surfactants (C), percent TRβ antagonism relative to intra-assay EC80 T3 for various ethoxylate chain length NPEOs (D), and inhibition or enhancement of cell viability relative to vehicle control for various ethoxylate chain length NPEOs (E). Data presented as mean ± SEM from 3 independent experiments. * indicates lowest concentration with significant antagonist activity relative to EC80 T3, p < .05, as per 1-way ANOVA and Dunnett’s posthoc test in GraphPad Prism 7.0.
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Ethoxylated Surfactants May Promote Adipogenesis via Inhibition of TRβ. Antagonist activity for the TRβ as measured via FRET reporter gene assay using the TRβ GeneBLAzer Reporter Assay as described in the Methods. Percent agonist TRβ activity for the T3 positive control relative to its maximum response (A), percent TRβ antagonism of intra-assay EC80 T3 (concentration that exhibits 80% of maximum T3 response; 0.3 nM) for various alkyl chain length ethoxylated surfactants (B), inhibition or enhancement of cell viability relative to vehicle control for various alkyl chain length ethoxylated surfactants (C), percent TRβ antagonism relative to intra-assay EC80 T3 for various ethoxylate chain length NPEOs (D), and inhibition or enhancement of cell viability relative to vehicle control for various ethoxylate chain length NPEOs (E). Data presented as mean ± SEM from 3 independent experiments. * indicates lowest concentration with significant antagonist activity relative to EC80 T3, p < .05, as per 1-way ANOVA and Dunnett’s posthoc test in GraphPad Prism 7.0.

Journal: Toxicological Sciences

Article Title: Nonionic Ethoxylated Surfactants Induce Adipogenesis in 3T3-L1 Cells

doi: 10.1093/toxsci/kfx234

Figure Lengend Snippet: Ethoxylated Surfactants May Promote Adipogenesis via Inhibition of TRβ. Antagonist activity for the TRβ as measured via FRET reporter gene assay using the TRβ GeneBLAzer Reporter Assay as described in the Methods. Percent agonist TRβ activity for the T3 positive control relative to its maximum response (A), percent TRβ antagonism of intra-assay EC80 T3 (concentration that exhibits 80% of maximum T3 response; 0.3 nM) for various alkyl chain length ethoxylated surfactants (B), inhibition or enhancement of cell viability relative to vehicle control for various alkyl chain length ethoxylated surfactants (C), percent TRβ antagonism relative to intra-assay EC80 T3 for various ethoxylate chain length NPEOs (D), and inhibition or enhancement of cell viability relative to vehicle control for various ethoxylate chain length NPEOs (E). Data presented as mean ± SEM from 3 independent experiments. * indicates lowest concentration with significant antagonist activity relative to EC80 T3, p < .05, as per 1-way ANOVA and Dunnett’s posthoc test in GraphPad Prism 7.0.

Article Snippet: EC20/50 values were estimated using curves generated from raw fluorescence data using a 4-parameter variable-slope Hill model in GraphPad Prism 7.0.

Techniques: Inhibition, Activity Assay, Reporter Gene Assay, Reporter Assay, Positive Control, Intra Assay, Concentration Assay