Journal: Saudi Journal of Biological Sciences

Article Title: Live attenuated influenza vaccine induces broadly cross-reactive mucosal antibody responses to different influenza strains in tonsils

doi: 10.1016/j.sjbs.2023.103809

Figure Lengend Snippet: LAIV did not induce anti-H7N3 HA IgG antibody production. No significant level of specific anti-HA IgG antibody to H7N3 was detected compared with unstimulated cells (n = 15, p > 0.05). Means and standard errors are shown.

Article Snippet: Purified recombinant HA proteins of pH1N1 (A/California/04/2009,VR-1894DQ),sH1N1 (A/Brisbane/59/2007, VR-1742), sH3N2 (A/Brisbane/10/2007, VR-1881), aH5N1 (A/Vietnam/1203/2004, VR-1931), A/Hong Kong/8/68, VR-1679), and H7N3 (A/Canada/RV444/04, VR-1641) were from Biodefense and Emerging Infections Research Resources Repository (BEI resources ATCC (Manassas VA, USA).

Techniques:

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Zinc treatment reverses and anti-Zn-regulated miRs suppress esophageal carcinomas in vivo.

doi: 10.1073/pnas.2220334120

Figure Lengend Snippet: Fig. 4. Zn treatment reverses ESCC metabolic phenotype in ZD rats. (A) Metabolomic profiling by GC-TOF MS on Zn-treated vs. Zn-untreated rat esophagus. A metabolomic network among the structurally identified metabolites was constructed (using the KEGG databases and PubChem compound identification [CID]) showing 14 up- and 24 downregulated metabolites. Each labeled node represents an identified and significantly altered metabolite (P < 0.05, t test; n = 10 rats per group). Blue and red nodes are significantly decreased and increased metabolites. The size of the nodes represents the size of the fold-change (Zn-treated vs. untreated). Blue lines are edges connected due to Tanimoto similarity; red lines are edges connected through KEGG reaction pairs. (B) Box and whisker plot (with median denoted by the line through the box and whiskers representing values within 1.5 IQR of the first and third quartile) showing distribution of putrescine and glucose metabolic activities and significantly reduced putrescine (P = 0.02) and increased glucose levels (P = 0.02) in Zn-treated vs. Zn-untreated esophagus. (C) IHC analysis of metabolite enzymes ODC (rate-limiting enzyme that converts ornithine to putrescine) expression and HK2 (rate-limiting enzyme and first obligatory step of glucose metabolism) expression in Zn-treated vs. Zn-untreated rat esophagus Representative esophageal photo showing moderate to strong immunostaining for ODC and HK2 in Zn-untreated esophagus as compared to weak and diffuse staining for the same protein in Zn-treated esophagus. HK2 and ODC expression (brown, 3,3′-diaminobenzidine tetrahydrochloride, DAB, counterstain, Harris modified hematoxylin). (D) Box and whisker plot showing distribution of ODC and HK2 immunoreactive score and significantly reduced ODC and reduced HK2 protein expression (P < 0.001) in Zn-treated vs. Zn-untreated esophagus.

Article Snippet: IHC was performed using primary antibodies for PCNA (clone PC-10, Ab-1, Thermo Scientific), COX-2 (#12282, Cell Signaling), S100A8 (T-1032, BMA, Augst, Switzerland), S100A9 (NB110-89726, Novus Biologicals), NF-κΒ p65 (ab7970, Abcam), STK40 (orb101780, Biorbyt), EGLN3 (orb443107, Biorbyt), PDCD4 (LS-B1388, Lifespan Biosciences, Seattle, WA), BAX (#14796, rabbit mAb, Cell Signaling), HK2 (NBP1-51643, Novus Biologicals), and ODC1 (NBP2-32887, Novus Biologicals).

Techniques: Construct, Labeling, Whisker Assay, Expressing, Immunostaining, Staining, Modification