Journal: Neurobiology of disease

Article Title: Ethanol exposure alters Alzheimer’s-related pathology, behavior, and metabolism in APP/PS1 mice

doi: 10.1016/j.nbd.2022.105967

Figure Lengend Snippet: Chronic moderate drinking differentially alters NMDA and GABAA receptors in the cortex and hippocampus of APP/PS1 mice. a) Ethanol treatment did not alter cortical Grin2a expression in wildtype or APP/PS1 mice. b) Ethanol-treated APP/PS1 mice had higher cortical Grin2b expression compared to EtOH-treated wildtype mice. 2-way ANOVA revealed a significant treatment × genotype interaction ( p = 0.0319). c) H 2 O-treated APP/PS1 mice showed increased cortical Gabra5 expression compared to H 2 O-exposed wildtype mice (p < 0.05). This effect was lost in EtOH-exposed APP/PS1 Gabra5 mRNA levels. 2-way ANOVA revealed a significant treatment × genotype interaction ( p = 0.0249) and a trend in genotype effects ( p = 0.0723). d) Synaptic GluN2A levels was unaltered in the hippocampus of H 2 O- or EtOH-treated wildtype or APP/PS1 mice. e) Synaptic GluN2B levels was unaltered in the hippocampus of H 2 O- or EtOH-treated wildtype or APP/PS1 mice. f) Ethanol-treated wildtype mice showed increased synaptic GABA A R α5 subunit levels compared to H 2 O-treated wildtype mice. Ethanol treatment had no effect on GABAAR α5 subunit levels in APP/PS1 mice. 2-way ANOVA revealed a significant treatment × genotype effect ( p = 0.0347) and a trend in treatment effects ( p = 0.0644). Wildtype + H2O, n = 10; APP/PS1 + H2O, n = 9; Wildtype + EtOH, n = 7; APP/PS1 + EtOH, n = 8. * p < 0.05.

Article Snippet: The following primary and secondary antibodies were used for this study: APP (including CTFβ and CTFα; Invitrogen; CT695; 1:1000), BACE1 (Cell Signaling; 5606S; 1:1000), ADAM10 (Millipore; AB19026; 1:1000), IDE (Abcam; ab232216; 1:1000), GluN2A (Cell Signaling; 4025; 1:1000), GluN2B (Cell Signaling; 4212; 1:1000), GABAAR α5 (Santa Cruz; Sc393921; 1:1000), and β-actin (Millipore; MAB1501; 1:50,000), anti-mouse (Cell Signaling; 7076S; 1:5000), anti-rabbit (Cell Signaling; 7074S; 1:5000).

Techniques: Expressing

Journal: Mediators of Inflammation

Article Title: Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) Downregulates the Expression of Protumor Factors Cyclooxygenase-2 and Inducible Nitric Oxide Synthase in a GM-CSF Receptor-Independent Manner in Cervical Cancer Cells

doi: 10.1155/2015/601604

Figure Lengend Snippet: Knockdown of GM-CSF upregulates iNOS and COX-2 expression. (a, b) Western blot analysis of Ca Ski, SiHa, and Hela cells transfected with siRNA targeting the GM-CSF gene to detect the expression of GM-CSF (a), iNOS and COX-2 (b), respectively. (c) Unpaired Student's t -test was used to detect the differences of iNOS and COX-2 expression in different groups. Each experiment was repeated three times. GT: GM-CSF siRNA transfection, VT: scrambled siRNA transfection, and NT: nontransfection ∗ p < 0.05 versus NT group. Error bars represent mean ± SD.

Article Snippet: To silence the expression of hGM-CSF, the cancer cell lines were seeded in 6-well tissue culture plates (10 5 cells/mL) and transfected using the Lipofectamine 2000 Reagent to introduce 5 nM of GM-CSF siRNA (number sc39391; Santa Cruz Biotech) or scrambled control siRNA (number sc-37007, Santa Cruz Biotech).

Techniques: Knockdown, Expressing, Western Blot, Transfection