38952 Search Results


h292 lung mucoepidermoid carcinoma cells  (ATCC)
90
ATCC h292 lung mucoepidermoid carcinoma cells
A549 cells were infected with Nkx2‐1 ‐expressing lentivirus as previously reported (Maeda et al , ). ChIP‐seq indicates that NKX2‐1 bound to the locus of PD‐L1 and PD‐L2 . PD‐L1 and PD‐L2 mRNAs were significantly induced by NKX2‐1. Results are expressed as mean ± SEM of biological triplicates for each group. P < 0.05 versus control was considered significant (Student's t ‐test). Gene expression was normalized by comparison with the constitutive expression of GAPDH . Control: control lentivirus; Nkx2‐1 : Nkx2‐1 ‐expressing lentivirus. A549 cells stably expressing Nkx2‐1 were developed as described in (A) and (B). Protein expression was confirmed by IB. PD‐L1 was detected by antibodies from Cell Signaling (CST), Spring Bioscience (Spring) and Sino Biological (Sino) as described in . ACTA1 was used as a loading control. Shown is a representative image from three independent experiments. NKX2‐1 induced PD‐L1 in A549 cells. H2122, Calu‐3, and <t>H292</t> mucus‐producing lung cancer cells were infected with control lentivirus or Nkx2‐1 ‐expressing lentivirus. Protein expression was confirmed by IB. PD‐L1 antibody from Cell Signaling (CST) was used to detect PD‐L1 protein. ACTA1 was used as a loading control. Shown is a representative image from two independent experiments. NKX2‐1 induced PD‐L1 in the mucus‐producing lung cancer cells. NKX2‐1 (black) was expressed in the nucleus of non‐mucinous lung tumor cells (non‐IMA) but not in mucinous lung tumor cells (IMA) in human. Scale bar: 50 μm. PD‐L1 was expressed in NKX2‐1‐positive lung tumor cells compared to NKX2‐1‐negative lung tumor cells in human (two‐tailed Fisher's exact test: P ‐value = 2.078E‐02). PD‐L1‐positive cases include > 5% of tumor cells expressing PD‐L1 in cell membrane. PD‐L1 (black) was expressed in cell membranes of non‐mucinous lung tumor cells (non‐IMA) but not in mucinous lung tumor cells (IMA) in human. Scale bar: 50 μm. PD‐L1 was expressed in non‐mucinous lung tumor cells (non‐IMA) but not in mucinous lung tumor cells (IMA) in human. PD‐L1‐positive cases were determined as described in (F). Source data are available online for this figure.
H292 Lung Mucoepidermoid Carcinoma Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/h292 lung mucoepidermoid carcinoma cells/product/ATCC
Average 90 stars, based on 1 article reviews
h292 lung mucoepidermoid carcinoma cells - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
camkii β  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology camkii β
A549 cells were infected with Nkx2‐1 ‐expressing lentivirus as previously reported (Maeda et al , ). ChIP‐seq indicates that NKX2‐1 bound to the locus of PD‐L1 and PD‐L2 . PD‐L1 and PD‐L2 mRNAs were significantly induced by NKX2‐1. Results are expressed as mean ± SEM of biological triplicates for each group. P < 0.05 versus control was considered significant (Student's t ‐test). Gene expression was normalized by comparison with the constitutive expression of GAPDH . Control: control lentivirus; Nkx2‐1 : Nkx2‐1 ‐expressing lentivirus. A549 cells stably expressing Nkx2‐1 were developed as described in (A) and (B). Protein expression was confirmed by IB. PD‐L1 was detected by antibodies from Cell Signaling (CST), Spring Bioscience (Spring) and Sino Biological (Sino) as described in . ACTA1 was used as a loading control. Shown is a representative image from three independent experiments. NKX2‐1 induced PD‐L1 in A549 cells. H2122, Calu‐3, and <t>H292</t> mucus‐producing lung cancer cells were infected with control lentivirus or Nkx2‐1 ‐expressing lentivirus. Protein expression was confirmed by IB. PD‐L1 antibody from Cell Signaling (CST) was used to detect PD‐L1 protein. ACTA1 was used as a loading control. Shown is a representative image from two independent experiments. NKX2‐1 induced PD‐L1 in the mucus‐producing lung cancer cells. NKX2‐1 (black) was expressed in the nucleus of non‐mucinous lung tumor cells (non‐IMA) but not in mucinous lung tumor cells (IMA) in human. Scale bar: 50 μm. PD‐L1 was expressed in NKX2‐1‐positive lung tumor cells compared to NKX2‐1‐negative lung tumor cells in human (two‐tailed Fisher's exact test: P ‐value = 2.078E‐02). PD‐L1‐positive cases include > 5% of tumor cells expressing PD‐L1 in cell membrane. PD‐L1 (black) was expressed in cell membranes of non‐mucinous lung tumor cells (non‐IMA) but not in mucinous lung tumor cells (IMA) in human. Scale bar: 50 μm. PD‐L1 was expressed in non‐mucinous lung tumor cells (non‐IMA) but not in mucinous lung tumor cells (IMA) in human. PD‐L1‐positive cases were determined as described in (F). Source data are available online for this figure.
Camkii β, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/camkii β/product/Santa Cruz Biotechnology
Average 93 stars, based on 1 article reviews
camkii β - by Bioz Stars, 2026-03
93/100 stars
  Buy from Supplier
lentiviral camkkb shrna constructs  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology lentiviral camkkb shrna constructs
A549 cells were infected with Nkx2‐1 ‐expressing lentivirus as previously reported (Maeda et al , ). ChIP‐seq indicates that NKX2‐1 bound to the locus of PD‐L1 and PD‐L2 . PD‐L1 and PD‐L2 mRNAs were significantly induced by NKX2‐1. Results are expressed as mean ± SEM of biological triplicates for each group. P < 0.05 versus control was considered significant (Student's t ‐test). Gene expression was normalized by comparison with the constitutive expression of GAPDH . Control: control lentivirus; Nkx2‐1 : Nkx2‐1 ‐expressing lentivirus. A549 cells stably expressing Nkx2‐1 were developed as described in (A) and (B). Protein expression was confirmed by IB. PD‐L1 was detected by antibodies from Cell Signaling (CST), Spring Bioscience (Spring) and Sino Biological (Sino) as described in . ACTA1 was used as a loading control. Shown is a representative image from three independent experiments. NKX2‐1 induced PD‐L1 in A549 cells. H2122, Calu‐3, and <t>H292</t> mucus‐producing lung cancer cells were infected with control lentivirus or Nkx2‐1 ‐expressing lentivirus. Protein expression was confirmed by IB. PD‐L1 antibody from Cell Signaling (CST) was used to detect PD‐L1 protein. ACTA1 was used as a loading control. Shown is a representative image from two independent experiments. NKX2‐1 induced PD‐L1 in the mucus‐producing lung cancer cells. NKX2‐1 (black) was expressed in the nucleus of non‐mucinous lung tumor cells (non‐IMA) but not in mucinous lung tumor cells (IMA) in human. Scale bar: 50 μm. PD‐L1 was expressed in NKX2‐1‐positive lung tumor cells compared to NKX2‐1‐negative lung tumor cells in human (two‐tailed Fisher's exact test: P ‐value = 2.078E‐02). PD‐L1‐positive cases include > 5% of tumor cells expressing PD‐L1 in cell membrane. PD‐L1 (black) was expressed in cell membranes of non‐mucinous lung tumor cells (non‐IMA) but not in mucinous lung tumor cells (IMA) in human. Scale bar: 50 μm. PD‐L1 was expressed in non‐mucinous lung tumor cells (non‐IMA) but not in mucinous lung tumor cells (IMA) in human. PD‐L1‐positive cases were determined as described in (F). Source data are available online for this figure.
Lentiviral Camkkb Shrna Constructs, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lentiviral camkkb shrna constructs/product/Santa Cruz Biotechnology
Average 93 stars, based on 1 article reviews
lentiviral camkkb shrna constructs - by Bioz Stars, 2026-03
93/100 stars
  Buy from Supplier
elisa sig-38952 betamark  (Covance)
90
Covance elisa sig-38952 betamark
A549 cells were infected with Nkx2‐1 ‐expressing lentivirus as previously reported (Maeda et al , ). ChIP‐seq indicates that NKX2‐1 bound to the locus of PD‐L1 and PD‐L2 . PD‐L1 and PD‐L2 mRNAs were significantly induced by NKX2‐1. Results are expressed as mean ± SEM of biological triplicates for each group. P < 0.05 versus control was considered significant (Student's t ‐test). Gene expression was normalized by comparison with the constitutive expression of GAPDH . Control: control lentivirus; Nkx2‐1 : Nkx2‐1 ‐expressing lentivirus. A549 cells stably expressing Nkx2‐1 were developed as described in (A) and (B). Protein expression was confirmed by IB. PD‐L1 was detected by antibodies from Cell Signaling (CST), Spring Bioscience (Spring) and Sino Biological (Sino) as described in . ACTA1 was used as a loading control. Shown is a representative image from three independent experiments. NKX2‐1 induced PD‐L1 in A549 cells. H2122, Calu‐3, and <t>H292</t> mucus‐producing lung cancer cells were infected with control lentivirus or Nkx2‐1 ‐expressing lentivirus. Protein expression was confirmed by IB. PD‐L1 antibody from Cell Signaling (CST) was used to detect PD‐L1 protein. ACTA1 was used as a loading control. Shown is a representative image from two independent experiments. NKX2‐1 induced PD‐L1 in the mucus‐producing lung cancer cells. NKX2‐1 (black) was expressed in the nucleus of non‐mucinous lung tumor cells (non‐IMA) but not in mucinous lung tumor cells (IMA) in human. Scale bar: 50 μm. PD‐L1 was expressed in NKX2‐1‐positive lung tumor cells compared to NKX2‐1‐negative lung tumor cells in human (two‐tailed Fisher's exact test: P ‐value = 2.078E‐02). PD‐L1‐positive cases include > 5% of tumor cells expressing PD‐L1 in cell membrane. PD‐L1 (black) was expressed in cell membranes of non‐mucinous lung tumor cells (non‐IMA) but not in mucinous lung tumor cells (IMA) in human. Scale bar: 50 μm. PD‐L1 was expressed in non‐mucinous lung tumor cells (non‐IMA) but not in mucinous lung tumor cells (IMA) in human. PD‐L1‐positive cases were determined as described in (F). Source data are available online for this figure.
Elisa Sig 38952 Betamark, supplied by Covance, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/elisa sig-38952 betamark/product/Covance
Average 90 stars, based on 1 article reviews
elisa sig-38952 betamark - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

Image Search Results


A549 cells were infected with Nkx2‐1 ‐expressing lentivirus as previously reported (Maeda et al , ). ChIP‐seq indicates that NKX2‐1 bound to the locus of PD‐L1 and PD‐L2 . PD‐L1 and PD‐L2 mRNAs were significantly induced by NKX2‐1. Results are expressed as mean ± SEM of biological triplicates for each group. P < 0.05 versus control was considered significant (Student's t ‐test). Gene expression was normalized by comparison with the constitutive expression of GAPDH . Control: control lentivirus; Nkx2‐1 : Nkx2‐1 ‐expressing lentivirus. A549 cells stably expressing Nkx2‐1 were developed as described in (A) and (B). Protein expression was confirmed by IB. PD‐L1 was detected by antibodies from Cell Signaling (CST), Spring Bioscience (Spring) and Sino Biological (Sino) as described in . ACTA1 was used as a loading control. Shown is a representative image from three independent experiments. NKX2‐1 induced PD‐L1 in A549 cells. H2122, Calu‐3, and H292 mucus‐producing lung cancer cells were infected with control lentivirus or Nkx2‐1 ‐expressing lentivirus. Protein expression was confirmed by IB. PD‐L1 antibody from Cell Signaling (CST) was used to detect PD‐L1 protein. ACTA1 was used as a loading control. Shown is a representative image from two independent experiments. NKX2‐1 induced PD‐L1 in the mucus‐producing lung cancer cells. NKX2‐1 (black) was expressed in the nucleus of non‐mucinous lung tumor cells (non‐IMA) but not in mucinous lung tumor cells (IMA) in human. Scale bar: 50 μm. PD‐L1 was expressed in NKX2‐1‐positive lung tumor cells compared to NKX2‐1‐negative lung tumor cells in human (two‐tailed Fisher's exact test: P ‐value = 2.078E‐02). PD‐L1‐positive cases include > 5% of tumor cells expressing PD‐L1 in cell membrane. PD‐L1 (black) was expressed in cell membranes of non‐mucinous lung tumor cells (non‐IMA) but not in mucinous lung tumor cells (IMA) in human. Scale bar: 50 μm. PD‐L1 was expressed in non‐mucinous lung tumor cells (non‐IMA) but not in mucinous lung tumor cells (IMA) in human. PD‐L1‐positive cases were determined as described in (F). Source data are available online for this figure.

Journal: EMBO Molecular Medicine

Article Title: Gene signature driving invasive mucinous adenocarcinoma of the lung

doi: 10.15252/emmm.201606711

Figure Lengend Snippet: A549 cells were infected with Nkx2‐1 ‐expressing lentivirus as previously reported (Maeda et al , ). ChIP‐seq indicates that NKX2‐1 bound to the locus of PD‐L1 and PD‐L2 . PD‐L1 and PD‐L2 mRNAs were significantly induced by NKX2‐1. Results are expressed as mean ± SEM of biological triplicates for each group. P < 0.05 versus control was considered significant (Student's t ‐test). Gene expression was normalized by comparison with the constitutive expression of GAPDH . Control: control lentivirus; Nkx2‐1 : Nkx2‐1 ‐expressing lentivirus. A549 cells stably expressing Nkx2‐1 were developed as described in (A) and (B). Protein expression was confirmed by IB. PD‐L1 was detected by antibodies from Cell Signaling (CST), Spring Bioscience (Spring) and Sino Biological (Sino) as described in . ACTA1 was used as a loading control. Shown is a representative image from three independent experiments. NKX2‐1 induced PD‐L1 in A549 cells. H2122, Calu‐3, and H292 mucus‐producing lung cancer cells were infected with control lentivirus or Nkx2‐1 ‐expressing lentivirus. Protein expression was confirmed by IB. PD‐L1 antibody from Cell Signaling (CST) was used to detect PD‐L1 protein. ACTA1 was used as a loading control. Shown is a representative image from two independent experiments. NKX2‐1 induced PD‐L1 in the mucus‐producing lung cancer cells. NKX2‐1 (black) was expressed in the nucleus of non‐mucinous lung tumor cells (non‐IMA) but not in mucinous lung tumor cells (IMA) in human. Scale bar: 50 μm. PD‐L1 was expressed in NKX2‐1‐positive lung tumor cells compared to NKX2‐1‐negative lung tumor cells in human (two‐tailed Fisher's exact test: P ‐value = 2.078E‐02). PD‐L1‐positive cases include > 5% of tumor cells expressing PD‐L1 in cell membrane. PD‐L1 (black) was expressed in cell membranes of non‐mucinous lung tumor cells (non‐IMA) but not in mucinous lung tumor cells (IMA) in human. Scale bar: 50 μm. PD‐L1 was expressed in non‐mucinous lung tumor cells (non‐IMA) but not in mucinous lung tumor cells (IMA) in human. PD‐L1‐positive cases were determined as described in (F). Source data are available online for this figure.

Article Snippet: A549 lung carcinoma cells (Lot# F‐10600), H2122 lung adenocarcinoma cells (Lot# 59399669), Calu‐3 lung adenocarcinoma cells (Lot# 57814093), and H292 lung mucoepidermoid carcinoma cells (Lot# 3895200) were obtained directly from ATCC (Manassas, VA).

Techniques: Infection, Expressing, ChIP-sequencing, Control, Gene Expression, Comparison, Stable Transfection, Two Tailed Test, Membrane