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Image Search Results
Journal: Annals of Translational Medicine
Article Title: Wogonin reverses the drug resistance of chronic myelogenous leukemia cells to imatinib through CXCL12-CXCR4/7 axis in bone marrow microenvironment
doi: 10.21037/atm-20-1166
Figure Lengend Snippet: The Id3 gene could regulate CXCR4 and CXCR7. (A) Id2 and Id3 genes in CML cells were investigated by RT-PCR in 10 ng TGF-β-treated or co-cultured with hBMSCs CML cells. (B) RT-PCR assay detection after CML cells being treated with Id2 siRNA or Id3 siRNA in CML cells. (C,D) Western blot analysis of CXCR4 and CXCR7 in CML cells treated with Id2 siRNA or Id3 siRNA with or without 10 ng TGF-β. *, P<0.05; **, P<0.01; ***, P<0.0001. hBMSCs, human bone marrow stromal cells; CML, chronic myeloid leukemia.
Article Snippet: The Id2 and
Techniques: Reverse Transcription Polymerase Chain Reaction, Cell Culture, Western Blot
Journal: Annals of Translational Medicine
Article Title: Wogonin reverses the drug resistance of chronic myelogenous leukemia cells to imatinib through CXCL12-CXCR4/7 axis in bone marrow microenvironment
doi: 10.21037/atm-20-1166
Figure Lengend Snippet: The TGF-β/Id3/CXCR4 and CXCR7 contributed to the reversal effects of Wogonin on IM-resistant CML cells. CML cells treated with Wogonin in co-culture model. (A) ELISA assay detection of TGF-β in supernatant. (B) Id3 gene in CML cells after co-culture with hBMSCs with or without 10 ng TGF-β-treated was investigated by RT-PCR assay. (C) Id3 gene in CML cells after Id3 over-expression. (D) ELISA assay detection of TGF-β in supernatant after Id3 over-expression with or without hBMSCs or 20 µM Wogonin. (E,F) Western blot analysis of CXCR4 and CXCR7 in CML cells after Id3 over-expression treated with or without hBMSCs or 20 µM Wogonin. *, P<0.05; **, P<0.01; ***, P<0.0001. hBMSCs, human bone marrow stromal cells; CML, chronic myeloid leukemia.
Article Snippet: The Id2 and
Techniques: Co-Culture Assay, Enzyme-linked Immunosorbent Assay, Reverse Transcription Polymerase Chain Reaction, Over Expression, Western Blot