Journal: Applied and Environmental Microbiology

Article Title: Norwalk-Like Virus Sequences in Mineral Waters: One-Year Monitoring of Three Brands

doi: 10.1128/aem.68.4.1925-1931.2002

Figure Lengend Snippet: FIG. 2. Agarose gel (2.6%) and ethidium bromide staining of RT-PCR products obtained with generic primers for NLV sequences (213-bp amplicon) in mineral water samples BAG 15 to BAG 24. Electrophoresis was carried out at 70 mV/cm. Samples 16 to 18, 21, 23, and 24 are NLV gg II sequence positive. P, positive control; N, negative controls; M, marker.

Article Snippet: Both strands of RT-PCR products were directly sequenced by cycle sequencing (ABI PRISM 377 DNA sequencer; Perkin-Elmer) carried out by Microsynth GmbH.

Techniques: Agarose Gel Electrophoresis, Staining, Reverse Transcription Polymerase Chain Reaction, Amplification, Electrophoresis, Sequencing, Positive Control, Marker

Journal: Applied and Environmental Microbiology

Article Title: Norwalk-Like Virus Sequences in Mineral Waters: One-Year Monitoring of Three Brands

doi: 10.1128/aem.68.4.1925-1931.2002

Figure Lengend Snippet: FIG. 3. Phylogenetic analyses performed on a 132-bp region of the RNA polymerase of NLV gg II sequences detected with genogroup-specific primers (12) by weekly analyses of 27 mineral water samples. An NLV gg II sequence of brand 3 isolated in the previous study (original designation, V2) (3), 4 of 53 identical positive-control sequences, and published sequences (accessed from GenBank) of Hawaii virus (U07611), Lordsdale virus (X86557), and Camberwell virus (AF145896) are also included. Three clusters of NLV sequences corresponding to each brand are shown.

Article Snippet: Both strands of RT-PCR products were directly sequenced by cycle sequencing (ABI PRISM 377 DNA sequencer; Perkin-Elmer) carried out by Microsynth GmbH.

Techniques: Sequencing, Isolation, Positive Control, Virus