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Image Search Results
Journal: PLoS Genetics
Article Title: Hsp70-nucleotide exchange factor (NEF) Fes1 has non-NEF roles in degradation of gluconeogenic enzymes and cell wall integrity
doi: 10.1371/journal.pgen.1008219
Figure Lengend Snippet: All panels show western analysis of indicated protein. (A) Fes1-GST was purified from lysates of fes1Δ cells expressing plasmid-encoded wild type Fes1 ( wt ), Fes1 A79R,R195A ( RA ) or Fes1ΔRD ( RD ) grown at 30°C with (+cfw) or without (-cfw) calcofluor white treatment for 60 min. Left (Input), whole lysates blotted and probed for Fes1 and Slt2. Right (Purified), GST purified proteins processed similarly. (B) Vid assay, as in , shows Vid is functional in cells lacking Slt2. Images labeled "load" show blotted membranes stained by amido-black as loading and transfer controls.
Article Snippet: For western analysis, lysates and purified proteins were separated on 12% SDS-PAGE gels, blotted using standard methods and probed using antibody against Fes1 (this study) or
Techniques: Western Blot, Purification, Expressing, Plasmid Preparation, Functional Assay, Labeling, Staining
Journal: PLoS Genetics
Article Title: Hsp70-nucleotide exchange factor (NEF) Fes1 has non-NEF roles in degradation of gluconeogenic enzymes and cell wall integrity
doi: 10.1371/journal.pgen.1008219
Figure Lengend Snippet: Strains used in this study.
Article Snippet: For western analysis, lysates and purified proteins were separated on 12% SDS-PAGE gels, blotted using standard methods and probed using antibody against Fes1 (this study) or
Techniques: