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Addgene inc
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Santa Cruz Biotechnology
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Image Search Results
Journal: Pediatric research
Article Title: Altered Prostanoid Metabolism Contributes to Impaired Angiogenesis in Persistent Pulmonary Hypertension of the Newborn
doi: 10.1038/pr.2014.209
Figure Lengend Snippet: Alterations in prostacyclin synthase (PGIS) protein levels and activity in pulmonary artery endothelial cells (PAEC) in persistent pulmonary hypertension of the newborn (PPHN). A: Levels of 6-Keto-PGF1α, a stable metabolite of PGI2, were decreased in PPHN (gray bar) at basal level and after ATP stimulation (n=12, p<0.05 from control PAEC) compared to control cells (filled bar). B:PGIS protein levels were decreased in PPHN (n=14, p <0.05). Representative western blots were shown with β-actin used as loading control. C:PGIS mRNA levels were not different between PPHN and control PAEC (n=7, p =0.12). D: PGIS nitration (N-PGIS) was higher in PPHN than control PAEC. (n=6, p <0.05).*Indicates p <0.05 compared to control PAEC.
Article Snippet:
Techniques: Activity Assay, Control, Western Blot, Nitration
Journal: Pediatric research
Article Title: Altered Prostanoid Metabolism Contributes to Impaired Angiogenesis in Persistent Pulmonary Hypertension of the Newborn
doi: 10.1038/pr.2014.209
Figure Lengend Snippet: A: PGIS knockdown with si-RNA decreases in vitro tube formation by control PAEC. Branch point number (n=4, p <0.05) and tube length (n=6, p <0.05) were decreased in control PAEC after application of PGIS-siRNA, compared to non-silencing (NS)-siRNA. Scale bar = 200 μ. B: PGIS si-RNA increased the gap in monolayer of control PAEC after a scratch, indicating longer recovery from injury compared to NS-RNA (n=10, p <0.05). Scale bar=100 μ. C: PGIS si-RNA also decreased the number of control cells able to invade through the Boyden chamber as compared to NS-siRNA treated control cells on cell invasion assay (n=4, p <0.05). For all studies, * indicates p <0.05 compared to NS-siRNA treated PAEC.
Article Snippet:
Techniques: Knockdown, In Vitro, Control, Invasion Assay