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92
Addgene inc lic cloning vector
Lic Cloning Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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85
Santa Cruz Biotechnology pgis sirna bovine
Alterations in <t>prostacyclin</t> <t>synthase</t> <t>(PGIS)</t> protein levels and activity in pulmonary artery endothelial cells (PAEC) in persistent pulmonary hypertension of the newborn (PPHN). A: Levels of 6-Keto-PGF1α, a stable metabolite of PGI2, were decreased in PPHN (gray bar) at basal level and after ATP stimulation (n=12, p<0.05 from control PAEC) compared to control cells (filled bar). B:PGIS protein levels were decreased in PPHN (n=14, p <0.05). Representative western blots were shown with β-actin used as loading control. C:PGIS mRNA levels were not different between PPHN and control PAEC (n=7, p =0.12). D: PGIS nitration (N-PGIS) was higher in PPHN than control PAEC. (n=6, p <0.05).*Indicates p <0.05 compared to control PAEC.
Pgis Sirna Bovine, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 85 stars, based on 1 article reviews
pgis sirna bovine - by Bioz Stars, 2026-02
85/100 stars
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Alterations in prostacyclin synthase (PGIS) protein levels and activity in pulmonary artery endothelial cells (PAEC) in persistent pulmonary hypertension of the newborn (PPHN). A: Levels of 6-Keto-PGF1α, a stable metabolite of PGI2, were decreased in PPHN (gray bar) at basal level and after ATP stimulation (n=12, p<0.05 from control PAEC) compared to control cells (filled bar). B:PGIS protein levels were decreased in PPHN (n=14, p <0.05). Representative western blots were shown with β-actin used as loading control. C:PGIS mRNA levels were not different between PPHN and control PAEC (n=7, p =0.12). D: PGIS nitration (N-PGIS) was higher in PPHN than control PAEC. (n=6, p <0.05).*Indicates p <0.05 compared to control PAEC.

Journal: Pediatric research

Article Title: Altered Prostanoid Metabolism Contributes to Impaired Angiogenesis in Persistent Pulmonary Hypertension of the Newborn

doi: 10.1038/pr.2014.209

Figure Lengend Snippet: Alterations in prostacyclin synthase (PGIS) protein levels and activity in pulmonary artery endothelial cells (PAEC) in persistent pulmonary hypertension of the newborn (PPHN). A: Levels of 6-Keto-PGF1α, a stable metabolite of PGI2, were decreased in PPHN (gray bar) at basal level and after ATP stimulation (n=12, p<0.05 from control PAEC) compared to control cells (filled bar). B:PGIS protein levels were decreased in PPHN (n=14, p <0.05). Representative western blots were shown with β-actin used as loading control. C:PGIS mRNA levels were not different between PPHN and control PAEC (n=7, p =0.12). D: PGIS nitration (N-PGIS) was higher in PPHN than control PAEC. (n=6, p <0.05).*Indicates p <0.05 compared to control PAEC.

Article Snippet: PGIS siRNA (bovine), TXAS siRNA (bovine), non-silencing siRNA (NS-siRNA) and Immunocruz IP/WB Optima F System were obtained from Santa Cruz biotechnology, Inc. (Santa Cruz, CA).

Techniques: Activity Assay, Control, Western Blot, Nitration

A: PGIS knockdown with si-RNA decreases in vitro tube formation by control PAEC. Branch point number (n=4, p <0.05) and tube length (n=6, p <0.05) were decreased in control PAEC after application of PGIS-siRNA, compared to non-silencing (NS)-siRNA. Scale bar = 200 μ. B: PGIS si-RNA increased the gap in monolayer of control PAEC after a scratch, indicating longer recovery from injury compared to NS-RNA (n=10, p <0.05). Scale bar=100 μ. C: PGIS si-RNA also decreased the number of control cells able to invade through the Boyden chamber as compared to NS-siRNA treated control cells on cell invasion assay (n=4, p <0.05). For all studies, * indicates p <0.05 compared to NS-siRNA treated PAEC.

Journal: Pediatric research

Article Title: Altered Prostanoid Metabolism Contributes to Impaired Angiogenesis in Persistent Pulmonary Hypertension of the Newborn

doi: 10.1038/pr.2014.209

Figure Lengend Snippet: A: PGIS knockdown with si-RNA decreases in vitro tube formation by control PAEC. Branch point number (n=4, p <0.05) and tube length (n=6, p <0.05) were decreased in control PAEC after application of PGIS-siRNA, compared to non-silencing (NS)-siRNA. Scale bar = 200 μ. B: PGIS si-RNA increased the gap in monolayer of control PAEC after a scratch, indicating longer recovery from injury compared to NS-RNA (n=10, p <0.05). Scale bar=100 μ. C: PGIS si-RNA also decreased the number of control cells able to invade through the Boyden chamber as compared to NS-siRNA treated control cells on cell invasion assay (n=4, p <0.05). For all studies, * indicates p <0.05 compared to NS-siRNA treated PAEC.

Article Snippet: PGIS siRNA (bovine), TXAS siRNA (bovine), non-silencing siRNA (NS-siRNA) and Immunocruz IP/WB Optima F System were obtained from Santa Cruz biotechnology, Inc. (Santa Cruz, CA).

Techniques: Knockdown, In Vitro, Control, Invasion Assay