37124 Search Results


jip3 sirna  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology jip3 sirna
FIG. 2. Interaction between <t>JIP3</t> and various types of the ASK1-MEK- JNK signaling proteins during glu- cose deprivation. DU-145 cells were co-infected with adenoviral vector containing FLAG-tagged JIP3 and HA- ASK1, His-MKK71, His-MKK71, His- MKK72, His-SEK1, HA-JNK1, or HA- JNK2 at an MOI of 10. After 48 h of infection, cells were exposed to glucose- free medium for various times (10–120 min). Cell lysates were immunoprecipi- tated (IP) with anti-FLAG antibody (A), anti-His antibody (B–F), or anti-HA anti- body (G) and immunoblotted (WB) with anti-FLAG, anti-His, or anti-HA antibody (upper panels). The presence of HA-ASK1 or FLAG-JIP3 in the lysates was verified by immunoblotting (lower panels).
Jip3 Sirna, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/jip3 sirna/product/Santa Cruz Biotechnology
Average 93 stars, based on 1 article reviews
jip3 sirna - by Bioz Stars, 2026-02
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plv105  (Addgene inc)
92
Addgene inc plv105
FIG. 2. Interaction between <t>JIP3</t> and various types of the ASK1-MEK- JNK signaling proteins during glu- cose deprivation. DU-145 cells were co-infected with adenoviral vector containing FLAG-tagged JIP3 and HA- ASK1, His-MKK71, His-MKK71, His- MKK72, His-SEK1, HA-JNK1, or HA- JNK2 at an MOI of 10. After 48 h of infection, cells were exposed to glucose- free medium for various times (10–120 min). Cell lysates were immunoprecipi- tated (IP) with anti-FLAG antibody (A), anti-His antibody (B–F), or anti-HA anti- body (G) and immunoblotted (WB) with anti-FLAG, anti-His, or anti-HA antibody (upper panels). The presence of HA-ASK1 or FLAG-JIP3 in the lysates was verified by immunoblotting (lower panels).
Plv105, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plv105/product/Addgene inc
Average 92 stars, based on 1 article reviews
plv105 - by Bioz Stars, 2026-02
92/100 stars
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an-37124  (Eli Lilly)
90
Eli Lilly an-37124
FIG. 2. Interaction between <t>JIP3</t> and various types of the ASK1-MEK- JNK signaling proteins during glu- cose deprivation. DU-145 cells were co-infected with adenoviral vector containing FLAG-tagged JIP3 and HA- ASK1, His-MKK71, His-MKK71, His- MKK72, His-SEK1, HA-JNK1, or HA- JNK2 at an MOI of 10. After 48 h of infection, cells were exposed to glucose- free medium for various times (10–120 min). Cell lysates were immunoprecipi- tated (IP) with anti-FLAG antibody (A), anti-His antibody (B–F), or anti-HA anti- body (G) and immunoblotted (WB) with anti-FLAG, anti-His, or anti-HA antibody (upper panels). The presence of HA-ASK1 or FLAG-JIP3 in the lysates was verified by immunoblotting (lower panels).
An 37124, supplied by Eli Lilly, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/an-37124/product/Eli Lilly
Average 90 stars, based on 1 article reviews
an-37124 - by Bioz Stars, 2026-02
90/100 stars
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Image Search Results


FIG. 2. Interaction between JIP3 and various types of the ASK1-MEK- JNK signaling proteins during glu- cose deprivation. DU-145 cells were co-infected with adenoviral vector containing FLAG-tagged JIP3 and HA- ASK1, His-MKK71, His-MKK71, His- MKK72, His-SEK1, HA-JNK1, or HA- JNK2 at an MOI of 10. After 48 h of infection, cells were exposed to glucose- free medium for various times (10–120 min). Cell lysates were immunoprecipi- tated (IP) with anti-FLAG antibody (A), anti-His antibody (B–F), or anti-HA anti- body (G) and immunoblotted (WB) with anti-FLAG, anti-His, or anti-HA antibody (upper panels). The presence of HA-ASK1 or FLAG-JIP3 in the lysates was verified by immunoblotting (lower panels).

Journal: Journal of Biological Chemistry

Article Title: Cross-talk between JIP3 and JIP1 during Glucose Deprivation

doi: 10.1074/jbc.m502318200

Figure Lengend Snippet: FIG. 2. Interaction between JIP3 and various types of the ASK1-MEK- JNK signaling proteins during glu- cose deprivation. DU-145 cells were co-infected with adenoviral vector containing FLAG-tagged JIP3 and HA- ASK1, His-MKK71, His-MKK71, His- MKK72, His-SEK1, HA-JNK1, or HA- JNK2 at an MOI of 10. After 48 h of infection, cells were exposed to glucose- free medium for various times (10–120 min). Cell lysates were immunoprecipi- tated (IP) with anti-FLAG antibody (A), anti-His antibody (B–F), or anti-HA anti- body (G) and immunoblotted (WB) with anti-FLAG, anti-His, or anti-HA antibody (upper panels). The presence of HA-ASK1 or FLAG-JIP3 in the lysates was verified by immunoblotting (lower panels).

Article Snippet: To down-regulate the JIP3, JIP3 siRNA (Santa Cruz Biotechnology) was used.

Techniques: Infection, Plasmid Preparation, Western Blot

FIG. 4. Role of JIP3 in the activation of SEK1 and JNK during glucose deprivation. DU-145 cells were transfected with JIP3 siRNA or mock siRNA. After 36 h of incubation, cells were exposed to glucose- free medium for 60 min. Cell lysates were immunoblotted (WB) with anti-JIP3, anti-ACTIVE JNK, anti-JNK2, anti-phospho-SEK1, anti- SEK1, or anti-actin antibody. Actin was used to confirm that similar amounts of proteins were loaded in each lane.

Journal: Journal of Biological Chemistry

Article Title: Cross-talk between JIP3 and JIP1 during Glucose Deprivation

doi: 10.1074/jbc.m502318200

Figure Lengend Snippet: FIG. 4. Role of JIP3 in the activation of SEK1 and JNK during glucose deprivation. DU-145 cells were transfected with JIP3 siRNA or mock siRNA. After 36 h of incubation, cells were exposed to glucose- free medium for 60 min. Cell lysates were immunoblotted (WB) with anti-JIP3, anti-ACTIVE JNK, anti-JNK2, anti-phospho-SEK1, anti- SEK1, or anti-actin antibody. Actin was used to confirm that similar amounts of proteins were loaded in each lane.

Article Snippet: To down-regulate the JIP3, JIP3 siRNA (Santa Cruz Biotechnology) was used.

Techniques: Activation Assay, Transfection, Incubation

FIG. 5. Role of ASK1 in the interaction between JIP3 and SEK1 during glucose deprivation. DU-145 cells were co-infected with adenoviral vector containing His-tagged SEK1 (Ad.His-SEK1), FLAG- tagged JIP3 (Ad.FLAG-JIP3), and HA-tagged ASK1 wild type (Ad.HA- ASK1WT) (A) or HA-tagged ASK1 kinase-inactive form K709M (Ad.HA- ASK1KM) (B) at an MOI of 10. After 48 h of infection, cells were exposed to glucose-free medium for various times (10–120 min). Cell lysates were immunoprecipitated (IP) with anti-His antibody and im- munoblotted (WB) with anti-FLAG or anti-His antibody (upper panels). The presence of FLAG-JIP3, HA-Akt1, phospho-JNK, JNK2, phospho- SEK1, or actin in the lysates was verified by immunoblotting (lower panels). Actin was used to confirm that similar amounts of proteins were loaded in each lane.

Journal: Journal of Biological Chemistry

Article Title: Cross-talk between JIP3 and JIP1 during Glucose Deprivation

doi: 10.1074/jbc.m502318200

Figure Lengend Snippet: FIG. 5. Role of ASK1 in the interaction between JIP3 and SEK1 during glucose deprivation. DU-145 cells were co-infected with adenoviral vector containing His-tagged SEK1 (Ad.His-SEK1), FLAG- tagged JIP3 (Ad.FLAG-JIP3), and HA-tagged ASK1 wild type (Ad.HA- ASK1WT) (A) or HA-tagged ASK1 kinase-inactive form K709M (Ad.HA- ASK1KM) (B) at an MOI of 10. After 48 h of infection, cells were exposed to glucose-free medium for various times (10–120 min). Cell lysates were immunoprecipitated (IP) with anti-His antibody and im- munoblotted (WB) with anti-FLAG or anti-His antibody (upper panels). The presence of FLAG-JIP3, HA-Akt1, phospho-JNK, JNK2, phospho- SEK1, or actin in the lysates was verified by immunoblotting (lower panels). Actin was used to confirm that similar amounts of proteins were loaded in each lane.

Article Snippet: To down-regulate the JIP3, JIP3 siRNA (Santa Cruz Biotechnology) was used.

Techniques: Infection, Plasmid Preparation, Immunoprecipitation, Western Blot

FIG. 6. Interaction between JIP3 and wild-type SEK1 or mu- tant-type SEK1 (T259A) and JNK activation in wild-type SEK1 (pHis-SEK1-WT) plasmid or mutant-type SEK1 (pHis-SEK1- T259A) transfected DU-145 cells during glucose deprivation. Cells were infected with Ad.FLAG-JIP3 at an MOI of 10 and transiently transfected with pHis-SEK1-WT (wild type) or pHis-SEK1-T259A (mu- tant type). After 48 h of incubation, cells were exposed to glucose-free medium for various times (30–120 min). Cell lysates were immunopre- cipitated with anti-His antibody and immunoblotted (WB) with anti- FLAG or anti-His antibody (upper panels). The presence of phospho- JNK, JNK2, FLAG-JIP3, or His-SEK1 in the lysates was verified by immunoblotting (lower panels).

Journal: Journal of Biological Chemistry

Article Title: Cross-talk between JIP3 and JIP1 during Glucose Deprivation

doi: 10.1074/jbc.m502318200

Figure Lengend Snippet: FIG. 6. Interaction between JIP3 and wild-type SEK1 or mu- tant-type SEK1 (T259A) and JNK activation in wild-type SEK1 (pHis-SEK1-WT) plasmid or mutant-type SEK1 (pHis-SEK1- T259A) transfected DU-145 cells during glucose deprivation. Cells were infected with Ad.FLAG-JIP3 at an MOI of 10 and transiently transfected with pHis-SEK1-WT (wild type) or pHis-SEK1-T259A (mu- tant type). After 48 h of incubation, cells were exposed to glucose-free medium for various times (30–120 min). Cell lysates were immunopre- cipitated with anti-His antibody and immunoblotted (WB) with anti- FLAG or anti-His antibody (upper panels). The presence of phospho- JNK, JNK2, FLAG-JIP3, or His-SEK1 in the lysates was verified by immunoblotting (lower panels).

Article Snippet: To down-regulate the JIP3, JIP3 siRNA (Santa Cruz Biotechnology) was used.

Techniques: Activation Assay, Plasmid Preparation, Mutagenesis, Transfection, Infection, Incubation, Western Blot

FIG. 12. A schematic model for the involvement of JIP3, SEK1/ MKK7, JNK1, JNK2, and JIP1 during glucose deprivation.

Journal: Journal of Biological Chemistry

Article Title: Cross-talk between JIP3 and JIP1 during Glucose Deprivation

doi: 10.1074/jbc.m502318200

Figure Lengend Snippet: FIG. 12. A schematic model for the involvement of JIP3, SEK1/ MKK7, JNK1, JNK2, and JIP1 during glucose deprivation.

Article Snippet: To down-regulate the JIP3, JIP3 siRNA (Santa Cruz Biotechnology) was used.

Techniques: