36388 Search Results


aaaag ctttt  (ATCC)
90
ATCC aaaag ctttt
Aaaag Ctttt, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/aaaag ctttt/product/ATCC
Average 90 stars, based on 1 article reviews
aaaag ctttt - by Bioz Stars, 2026-02
90/100 stars
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rap1gap short hairpin rna shrna plasmids  (Santa Cruz Biotechnology)
90
Santa Cruz Biotechnology rap1gap short hairpin rna shrna plasmids
Figure 4. Role of <t>RAP1GAP</t> in growth of primary and metastatic melanoma cells and subcellular distribution of EPAC1/2 signaling proteins. A, Western blot analysis showing endogenous levels of RAP1GAP protein in a panel of matched primary and metastatic melanoma cells. GAPDH is used as loading control. Numbers on the right indicate the molecular weight (kDa) of the respective proteins. B, Western blot analysis for validation of RAP1GAP KD. Matched primary and metastatic melanoma cells were transduced with RAP1GAP shRNA or scrambled shRNA control lentivirus and selected in puromycin containing medium for 4 days. Protein lysates were analyzed for RAP1GAP, total and active RAP1 (RAP1-GTP). C, Effect of RAP1GAP KD and ESI-09 treatment on the growth of primary and metastatic melanoma cells. Data (mean SD) from three replicates analyzed by unpaired Student t test are shown. , P value ≤0.05; , P ≤0.01; , P ≤0.001; and , P ≤0.0001. D, Western blot analysis showing subcellular distribution of EPAC1/2, RAP1-GTP, and RAP1GAP in matched primary (WM115) and metastatic (WM165-1) melanoma cells. GAPDH, Na/K-ATPase, and HDAC1 were used as makers of cytosolic (C), membrane (M), and nuclear (N) fractions, respectively. Numbers on the right indicate the molecular weight (kDa) of the respective proteins.
Rap1gap Short Hairpin Rna Shrna Plasmids, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rap1gap short hairpin rna shrna plasmids/product/Santa Cruz Biotechnology
Average 90 stars, based on 1 article reviews
rap1gap short hairpin rna shrna plasmids - by Bioz Stars, 2026-02
90/100 stars
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psmp ezh2 2  (Addgene inc)
94
Addgene inc psmp ezh2 2
Figure 4. Role of <t>RAP1GAP</t> in growth of primary and metastatic melanoma cells and subcellular distribution of EPAC1/2 signaling proteins. A, Western blot analysis showing endogenous levels of RAP1GAP protein in a panel of matched primary and metastatic melanoma cells. GAPDH is used as loading control. Numbers on the right indicate the molecular weight (kDa) of the respective proteins. B, Western blot analysis for validation of RAP1GAP KD. Matched primary and metastatic melanoma cells were transduced with RAP1GAP shRNA or scrambled shRNA control lentivirus and selected in puromycin containing medium for 4 days. Protein lysates were analyzed for RAP1GAP, total and active RAP1 (RAP1-GTP). C, Effect of RAP1GAP KD and ESI-09 treatment on the growth of primary and metastatic melanoma cells. Data (mean SD) from three replicates analyzed by unpaired Student t test are shown. , P value ≤0.05; , P ≤0.01; , P ≤0.001; and , P ≤0.0001. D, Western blot analysis showing subcellular distribution of EPAC1/2, RAP1-GTP, and RAP1GAP in matched primary (WM115) and metastatic (WM165-1) melanoma cells. GAPDH, Na/K-ATPase, and HDAC1 were used as makers of cytosolic (C), membrane (M), and nuclear (N) fractions, respectively. Numbers on the right indicate the molecular weight (kDa) of the respective proteins.
Psmp Ezh2 2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/psmp ezh2 2/product/Addgene inc
Average 94 stars, based on 1 article reviews
psmp ezh2 2 - by Bioz Stars, 2026-02
94/100 stars
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Image Search Results


Figure 4. Role of RAP1GAP in growth of primary and metastatic melanoma cells and subcellular distribution of EPAC1/2 signaling proteins. A, Western blot analysis showing endogenous levels of RAP1GAP protein in a panel of matched primary and metastatic melanoma cells. GAPDH is used as loading control. Numbers on the right indicate the molecular weight (kDa) of the respective proteins. B, Western blot analysis for validation of RAP1GAP KD. Matched primary and metastatic melanoma cells were transduced with RAP1GAP shRNA or scrambled shRNA control lentivirus and selected in puromycin containing medium for 4 days. Protein lysates were analyzed for RAP1GAP, total and active RAP1 (RAP1-GTP). C, Effect of RAP1GAP KD and ESI-09 treatment on the growth of primary and metastatic melanoma cells. Data (mean SD) from three replicates analyzed by unpaired Student t test are shown. , P value ≤0.05; , P ≤0.01; , P ≤0.001; and , P ≤0.0001. D, Western blot analysis showing subcellular distribution of EPAC1/2, RAP1-GTP, and RAP1GAP in matched primary (WM115) and metastatic (WM165-1) melanoma cells. GAPDH, Na/K-ATPase, and HDAC1 were used as makers of cytosolic (C), membrane (M), and nuclear (N) fractions, respectively. Numbers on the right indicate the molecular weight (kDa) of the respective proteins.

Journal: Molecular Cancer Research

Article Title: EPAC Regulates Melanoma Growth by Stimulating mTORC1 Signaling and Loss of EPAC Signaling Dependence Correlates with Melanoma Progression

doi: 10.1158/1541-7786.mcr-22-0026

Figure Lengend Snippet: Figure 4. Role of RAP1GAP in growth of primary and metastatic melanoma cells and subcellular distribution of EPAC1/2 signaling proteins. A, Western blot analysis showing endogenous levels of RAP1GAP protein in a panel of matched primary and metastatic melanoma cells. GAPDH is used as loading control. Numbers on the right indicate the molecular weight (kDa) of the respective proteins. B, Western blot analysis for validation of RAP1GAP KD. Matched primary and metastatic melanoma cells were transduced with RAP1GAP shRNA or scrambled shRNA control lentivirus and selected in puromycin containing medium for 4 days. Protein lysates were analyzed for RAP1GAP, total and active RAP1 (RAP1-GTP). C, Effect of RAP1GAP KD and ESI-09 treatment on the growth of primary and metastatic melanoma cells. Data (mean SD) from three replicates analyzed by unpaired Student t test are shown. , P value ≤0.05; , P ≤0.01; , P ≤0.001; and , P ≤0.0001. D, Western blot analysis showing subcellular distribution of EPAC1/2, RAP1-GTP, and RAP1GAP in matched primary (WM115) and metastatic (WM165-1) melanoma cells. GAPDH, Na/K-ATPase, and HDAC1 were used as makers of cytosolic (C), membrane (M), and nuclear (N) fractions, respectively. Numbers on the right indicate the molecular weight (kDa) of the respective proteins.

Article Snippet: HEK293 cells were transfected with a control empty vector pLKO.1 (SHC001V) or a pool of three RAP1GAP short hairpin RNA (shRNA) plasmids (sc-36388-SH; Santa Cruz Biotechnology).

Techniques: Western Blot, Control, Molecular Weight, Biomarker Discovery, Transduction, shRNA, Membrane