36296 Search Results


pp1c  (Santa Cruz Biotechnology)
90
Santa Cruz Biotechnology pp1c
FIGURE 5. Effects of Ser/Thr protein phosphatase inhibitors or knock- down of <t>PP1c</t> isoforms with siRNAs on PKC/II phosphorylation at Thr- 638/641. A, MCF-7 cells were treated with vehicle (EtOH, ethanol), 1 nM tau- tomycin (TAU), or 1 nM okadaic acid (OKA) for 6 h followed by 1 h of treatment with 100 nM PMA. B, MCF-7 cells were transfected with scrambled siRNA (SCR) or siRNAs specific for PP1c-, PP1c-, PP1c-, or PP2Ac-. Transfected cells were then stimulated with 100 nM PMA for 1 h and harvested. Levels of PKC or phosphorylated PKC/II at Thr-638/641 were determined as described under “Experimental Procedures”.
Pp1c, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/36296/10__1074_slash_jbc__m609162200-124-6-10?v=Santa+Cruz+Biotechnology
Average 90 stars, based on 1 article reviews
pp1c - by Bioz Stars, 2026-08
90/100 stars
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microsporum canis  (ATCC)
92
ATCC microsporum canis
FIGURE 5. Effects of Ser/Thr protein phosphatase inhibitors or knock- down of <t>PP1c</t> isoforms with siRNAs on PKC/II phosphorylation at Thr- 638/641. A, MCF-7 cells were treated with vehicle (EtOH, ethanol), 1 nM tau- tomycin (TAU), or 1 nM okadaic acid (OKA) for 6 h followed by 1 h of treatment with 100 nM PMA. B, MCF-7 cells were transfected with scrambled siRNA (SCR) or siRNAs specific for PP1c-, PP1c-, PP1c-, or PP2Ac-. Transfected cells were then stimulated with 100 nM PMA for 1 h and harvested. Levels of PKC or phosphorylated PKC/II at Thr-638/641 were determined as described under “Experimental Procedures”.
Microsporum Canis, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/36296/pmc03406219-3810-39-44?v=ATCC
Average 92 stars, based on 1 article reviews
microsporum canis - by Bioz Stars, 2026-08
92/100 stars
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Image Search Results


FIGURE 5. Effects of Ser/Thr protein phosphatase inhibitors or knock- down of PP1c isoforms with siRNAs on PKC/II phosphorylation at Thr- 638/641. A, MCF-7 cells were treated with vehicle (EtOH, ethanol), 1 nM tau- tomycin (TAU), or 1 nM okadaic acid (OKA) for 6 h followed by 1 h of treatment with 100 nM PMA. B, MCF-7 cells were transfected with scrambled siRNA (SCR) or siRNAs specific for PP1c-, PP1c-, PP1c-, or PP2Ac-. Transfected cells were then stimulated with 100 nM PMA for 1 h and harvested. Levels of PKC or phosphorylated PKC/II at Thr-638/641 were determined as described under “Experimental Procedures”.

Journal: Journal of Biological Chemistry

Article Title: Mechanism of Inhibition of Sequestration of Protein Kinase C α/βII by Ceramide

doi: 10.1074/jbc.m609162200

Figure Lengend Snippet: FIGURE 5. Effects of Ser/Thr protein phosphatase inhibitors or knock- down of PP1c isoforms with siRNAs on PKC/II phosphorylation at Thr- 638/641. A, MCF-7 cells were treated with vehicle (EtOH, ethanol), 1 nM tau- tomycin (TAU), or 1 nM okadaic acid (OKA) for 6 h followed by 1 h of treatment with 100 nM PMA. B, MCF-7 cells were transfected with scrambled siRNA (SCR) or siRNAs specific for PP1c-, PP1c-, PP1c-, or PP2Ac-. Transfected cells were then stimulated with 100 nM PMA for 1 h and harvested. Levels of PKC or phosphorylated PKC/II at Thr-638/641 were determined as described under “Experimental Procedures”.

Article Snippet: Specific siRNAs for PP2Ac- (sc-36301) and PP1c- (sc-36295) were from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA).

Techniques: Knockdown, Phospho-proteomics, Transfection

FIGURE 6. Involvement of Ser/Thr protein phosphatases in GFP-PKC sequestration to pericentrion in MCF-7 cells. A, MCF-7 cells transfected with GFP-PKC were pretreated with the indicated concentrations of tautomycin (TAU) or okadaic acid (OKA) for 6 h followed by 1 h of treatment with 100 nM PMA. After treatment cells were fixed, and translocation of GFP-PKC was analyzed by confocal microscopy. GFP-PKC translocation was assessed by blinded quantification of confocal microscopy pictures. B, MCF-7 cells were transfected with siRNAs (scrambled RNA (SCR), PP1c-, PP1c-, PP1c-, and PP2Ac-) for 48 h followed by transfection with GFP-PKC for 12 h. Cells were stimulated with 100 nM PMA or 0.01% Me2SO (control) for 1 h, fixed, and analyzed by confocal microscopy. C, GFP-PKC translocation was assessed by blinded quantification of confocal micros- copy pictures.

Journal: Journal of Biological Chemistry

Article Title: Mechanism of Inhibition of Sequestration of Protein Kinase C α/βII by Ceramide

doi: 10.1074/jbc.m609162200

Figure Lengend Snippet: FIGURE 6. Involvement of Ser/Thr protein phosphatases in GFP-PKC sequestration to pericentrion in MCF-7 cells. A, MCF-7 cells transfected with GFP-PKC were pretreated with the indicated concentrations of tautomycin (TAU) or okadaic acid (OKA) for 6 h followed by 1 h of treatment with 100 nM PMA. After treatment cells were fixed, and translocation of GFP-PKC was analyzed by confocal microscopy. GFP-PKC translocation was assessed by blinded quantification of confocal microscopy pictures. B, MCF-7 cells were transfected with siRNAs (scrambled RNA (SCR), PP1c-, PP1c-, PP1c-, and PP2Ac-) for 48 h followed by transfection with GFP-PKC for 12 h. Cells were stimulated with 100 nM PMA or 0.01% Me2SO (control) for 1 h, fixed, and analyzed by confocal microscopy. C, GFP-PKC translocation was assessed by blinded quantification of confocal micros- copy pictures.

Article Snippet: Specific siRNAs for PP2Ac- (sc-36301) and PP1c- (sc-36295) were from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA).

Techniques: Transfection, Translocation Assay, Confocal Microscopy, Control