Journal: Molecular Cancer

Article Title: HnRPD/AUF1 facilitates human ovarian cancer progression through activating FLI1 and maintaining cisplatin resistance

doi: 10.1186/s12943-026-02599-5

Figure Lengend Snippet: HnRPD transactivates GPR137 through FLI1. A IHC staining for FLI1 in paraffin-embedded sections of cancer tissues and adjacent normal epithelial tissues from patients with OC. N = 10; bar, 20 μm. B IHC score was analyzed. N = 10. ** p < 0.01 versus Nor. C Comparison of FLI1 protein expression in OC stage 1 (n = 1), stage 2 (n = 20), stage 3 (n = 243) and stage 4 (n = 38). D Kaplan-Meier analysis of overall survival for OC patients with altered expression of FLI1. E The protein expression of FLI1 in different OC cell lines. F CCK-8 assays of A2780 cells transfected with a FLI1-expressing vector or an empty vector (Control) and cultured for the indicated time periods. ** p < 0.01 versus Control; error bar, SD. N = 3. G Wound healing assays of A2780 cells transfected with a FLI1-expressing vector or an empty vector (Control) at 24 h. H Migrated distance was statistically analyzed. Bar, 100 μm. ** p < 0.01 versus Con; error bar, SD. N = 3. I Matrigel invasion assays of A2780 cells transfected with a FLI1-expressing vector or an empty vector (Control) at 24 h. Bar, 100 μm. J Quantitative analysis of (I). ** p < 0.01 versus Con; error bar, SD. N = 3. K Colony formation assays of A2780 cells infected with lentiviruses expressing FLI1 or control lentiviruses. L Quantitative analysis of (K). ** p < 0.01 versus Con; error bar, SD. N = 3. M Apoptotic ratios of A2780 cells transfected with a FLI1-expressing vector or an empty vector (Control) at 24 h. N Quantitative analysis of (M). ** p < 0.01 versus Con; error bar, SD. N = 3. O Nude mice with xenografts derived from A2780 cells stably expressing FLI1 or control. N = 6. P Weights of xenografts derived from A2780 cells stably expressing FLI1 or control. N = 6. ** p < 0.01 versus Con; error bar, SD. Q Tumor volume was measured on day 18 after A2780 cell injection. N = 6. ** p < 0.01 versus Con; error bar, SD. R KI-67 staining of tumor tissues derived from A2780 cells stably expressing FLI1 or control. Bar, 15 μm. S The mRNA expression of FLI1 in SK-OV-3 cells transfected with hnRPD shRNA or scrambled shRNA (shRNA-Con). ** p < 0.01 versus shRNA-Con; error bar, SD. N = 3. T The mRNA expression of FLI1 in OVCAR-8 cells transfected with a vector expressing hnRPD or a control empty vector (Con). ** p < 0.01 versus Con; error bar, SD. N = 3. U The protein expression of FLI1 in SK-OV-3 cells transfected with hnRPD shRNA or scrambled shRNA (shRNA-Con). V The protein expression of FLI1 in OVCAR-8 cells transfected with a vector expressing hnRPD or a control empty vector (Con). W The mRNA expression of GPR137 in A2780 cells transfected with a vector expressing FLI1 or a control empty vector (Con). ** p < 0.01 versus Con; error bar, SD. N = 3. X The mRNA expression of GPR137 in SK-OV-3 cells transfected with FLI1 shRNA or scrambled shRNA (shRNA-Con). ** p < 0.01 versus shRNA-Con; error bar, SD. N = 3. Y RT-PCR results showing the mRNA levels of FLI1 in OVCAR-8 cells transfected with a vector expressing hnRPD or a control empty vector (Con) and cultured with actinomycin D (AcD) for the indicated time periods. Z The mRNA levels of FLI1 in OVCAR-8 cells transfected with a vector expressing hnRPD or a control empty vector (Con) and cultured with actinomycin D (AcD) for the indicated time periods. ** p < 0.01 versus Con; error bar, SD. N = 3. AA GPR137 -Luc activities in A2780 cells transfected with FLI1 or a control empty vector. ** p < 0.01 versus Con; error bar, SD. N = 3. AB GPR137 -Luc activities in SK-OV-3 cells transfected with FLI1 shRNA (shRNA FLI1) or scrambled shRNA (shRNA Con). ** p < 0.01 versus shRNA-Con; error bar, SD. N = 3. AC Detection of the possible interaction between FLI1 and GPR137 promoter region by ChIP assays. IgG and a hnRPD antibody were used negative controls. N = 3. AD The FLI1-binding motif(s) located within GPR137 promoter was predicted. AE Mutated- GPR137 -Luc activities in A2780 cells transfected with FLI1 or a control empty vector. Error bar, SD. N = 3. AF Detection of the possible interaction between FLI1 and GPR137 promoter regions including -1600/-1500, -1528/-1429, and -1639/-1540 by ChIP assays. N = 3. AG The mRNA expression of GPR137 in OVCAR-8 cells transfected with a vector expressing hnRPD or a control empty vector (Con) in combination with FLI1-shRNA or Scrambled shRNA. ** p < 0.01 versus shRNA-Con+Control, ## p < 0.01 versus shRNA-Con+hnRPD; error bar, SD. N = 3. AH GPR137 -Luc activities in OVCAR-8 cells transfected with a vector expressing hnRPD or a control empty vector (Con) in combination with FLI1-shRNA or Scrambled shRNA. ** p < 0.01 versus shRNA-Con+Control, ## p < 0.01 versus shRNA-Con+hnRPD; error bar, SD. N = 3. AI Image showing the size of the tumor xenografts from three groups. AJ Weights of xenografts derived from the three groups. N = 6. ** p < 0.01 versus shRNA-Con+Control, ## p < 0.01 versus shRNA-Con+hnRPD; error bar, SD

Article Snippet: FLI1 antibody (#35980) and normal rabbit IgG (#2729) were from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Immunohistochemistry, Comparison, Expressing, CCK-8 Assay, Transfection, Plasmid Preparation, Control, Cell Culture, Infection, Derivative Assay, Stable Transfection, Injection, Staining, shRNA, Reverse Transcription Polymerase Chain Reaction, Binding Assay