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ATCC
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Journal: PLoS ONE
Article Title: Functional interaction between co-expressed MAGE-A proteins
doi: 10.1371/journal.pone.0178370
Figure Lengend Snippet: (A) Reporter gene assay for GR, MR and AR activity using specific gene-reporter in the presence or absence of MageA6 or MageA11 expression. Cells were treated with dexamethasone (Dx), Aldosterone (Aldo) or dihidrotestosterone (DHT) for 24 h prior to harvesting. The assay was performed in HEK293T cells. ev, empty vector. (B) Similar to A but combining MageA11 and MageA6 expression as indicated. (C) Determination of PSA mRNA levels through RT-qPCR. LNCaP cells were transfected with a siRNA control (siC) or a siRNA to silence MageA6 expression (siA6/2). DHT was added to cells as indicated. PSA mRNA levels were normalized to GAPDH mRNA levels. Error bars indicate mean S.D. Student’s t test was used for statistical analysis. * p < 0.05. ** p < 0.001.
Article Snippet: For MageA6 silencing we used
Techniques: Reporter Gene Assay, Activity Assay, Expressing, Plasmid Preparation, Quantitative RT-PCR, Transfection, Control