35440 Search Results


well collagen  (ATCC)
92
ATCC well collagen
Well Collagen, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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well collagen - by Bioz Stars, 2026-02
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anti ythdc2  (Cell Signaling Technology Inc)
93
Cell Signaling Technology Inc anti ythdc2
Anti Ythdc2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti ythdc2/product/Cell Signaling Technology Inc
Average 93 stars, based on 1 article reviews
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gadd 45α sirna plasmid  (Santa Cruz Biotechnology)
94
Santa Cruz Biotechnology gadd 45α sirna plasmid
GADD45A , MYC and TOP2A are responsible for the doxorubicin resistance induced by SNORD3A , SNORA13 and SNORA28 . U-2OS cells were transfected with an expression vector for SNORD3A , SNORA13 or SNORA28 . When indicated, cells were transiently transfected with a non-targeting scrambled <t>siRNA</t> pool (scr), with a GADD45A - or MYC -targeting siRNA (siGADD45A/siMYC) pool, with an empty vector (em) or with an expression vector for TOP2A (overTOP2A). U-2OS/DX30 and U-2OS/DX100 cells were included as Dox-resistant cells. Cells were treated with 5 μM Dox for 24 h (panel B ) or 72 h (panel C ). ( A ) mRNA levels of GADD45A , MYC and TOP2 A, evaluated by RT-PCR, in triplicates. Data are means ± SD ( n = 3). ** p < 0.01, *** p < 0.001: U-2OS/DX30, U-2OS/DX100 or snoRNA-over-expressing U-2OS cells vs. U-2OS cells; °° p < 0.01, °°° p < 0.001: siGADD45A/siMYC vs. scr cells, overTOP2A vs. em cells. ( B ) Release of LDH, an index of cell necrosis, measured spectrophotometrically, in duplicates. Data are means ± SD ( n = 3). *** p < 0.001: Dox-treated U-2OS cells vs. untreated (-Dox) cells. ( C ) Representative photographs of crystal violet staining, used as assay of cell viability. The photograph is representative of one out of four experiments, in quadruplicates.
Gadd 45α Sirna Plasmid, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gadd 45α sirna plasmid/product/Santa Cruz Biotechnology
Average 94 stars, based on 1 article reviews
gadd 45α sirna plasmid - by Bioz Stars, 2026-02
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prsii415  (Addgene inc)
93
Addgene inc prsii415
GADD45A , MYC and TOP2A are responsible for the doxorubicin resistance induced by SNORD3A , SNORA13 and SNORA28 . U-2OS cells were transfected with an expression vector for SNORD3A , SNORA13 or SNORA28 . When indicated, cells were transiently transfected with a non-targeting scrambled <t>siRNA</t> pool (scr), with a GADD45A - or MYC -targeting siRNA (siGADD45A/siMYC) pool, with an empty vector (em) or with an expression vector for TOP2A (overTOP2A). U-2OS/DX30 and U-2OS/DX100 cells were included as Dox-resistant cells. Cells were treated with 5 μM Dox for 24 h (panel B ) or 72 h (panel C ). ( A ) mRNA levels of GADD45A , MYC and TOP2 A, evaluated by RT-PCR, in triplicates. Data are means ± SD ( n = 3). ** p < 0.01, *** p < 0.001: U-2OS/DX30, U-2OS/DX100 or snoRNA-over-expressing U-2OS cells vs. U-2OS cells; °° p < 0.01, °°° p < 0.001: siGADD45A/siMYC vs. scr cells, overTOP2A vs. em cells. ( B ) Release of LDH, an index of cell necrosis, measured spectrophotometrically, in duplicates. Data are means ± SD ( n = 3). *** p < 0.001: Dox-treated U-2OS cells vs. untreated (-Dox) cells. ( C ) Representative photographs of crystal violet staining, used as assay of cell viability. The photograph is representative of one out of four experiments, in quadruplicates.
Prsii415, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/prsii415/product/Addgene inc
Average 93 stars, based on 1 article reviews
prsii415 - by Bioz Stars, 2026-02
93/100 stars
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gadd45α  (Santa Cruz Biotechnology)
91
Santa Cruz Biotechnology gadd45α
GADD45A , MYC and TOP2A are responsible for the doxorubicin resistance induced by SNORD3A , SNORA13 and SNORA28 . U-2OS cells were transfected with an expression vector for SNORD3A , SNORA13 or SNORA28 . When indicated, cells were transiently transfected with a non-targeting scrambled <t>siRNA</t> pool (scr), with a GADD45A - or MYC -targeting siRNA (siGADD45A/siMYC) pool, with an empty vector (em) or with an expression vector for TOP2A (overTOP2A). U-2OS/DX30 and U-2OS/DX100 cells were included as Dox-resistant cells. Cells were treated with 5 μM Dox for 24 h (panel B ) or 72 h (panel C ). ( A ) mRNA levels of GADD45A , MYC and TOP2 A, evaluated by RT-PCR, in triplicates. Data are means ± SD ( n = 3). ** p < 0.01, *** p < 0.001: U-2OS/DX30, U-2OS/DX100 or snoRNA-over-expressing U-2OS cells vs. U-2OS cells; °° p < 0.01, °°° p < 0.001: siGADD45A/siMYC vs. scr cells, overTOP2A vs. em cells. ( B ) Release of LDH, an index of cell necrosis, measured spectrophotometrically, in duplicates. Data are means ± SD ( n = 3). *** p < 0.001: Dox-treated U-2OS cells vs. untreated (-Dox) cells. ( C ) Representative photographs of crystal violet staining, used as assay of cell viability. The photograph is representative of one out of four experiments, in quadruplicates.
Gadd45α, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gadd45α/product/Santa Cruz Biotechnology
Average 91 stars, based on 1 article reviews
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blower model jabsco-35440  (Jabsco GmbH)
90
Jabsco GmbH blower model jabsco-35440
GADD45A , MYC and TOP2A are responsible for the doxorubicin resistance induced by SNORD3A , SNORA13 and SNORA28 . U-2OS cells were transfected with an expression vector for SNORD3A , SNORA13 or SNORA28 . When indicated, cells were transiently transfected with a non-targeting scrambled <t>siRNA</t> pool (scr), with a GADD45A - or MYC -targeting siRNA (siGADD45A/siMYC) pool, with an empty vector (em) or with an expression vector for TOP2A (overTOP2A). U-2OS/DX30 and U-2OS/DX100 cells were included as Dox-resistant cells. Cells were treated with 5 μM Dox for 24 h (panel B ) or 72 h (panel C ). ( A ) mRNA levels of GADD45A , MYC and TOP2 A, evaluated by RT-PCR, in triplicates. Data are means ± SD ( n = 3). ** p < 0.01, *** p < 0.001: U-2OS/DX30, U-2OS/DX100 or snoRNA-over-expressing U-2OS cells vs. U-2OS cells; °° p < 0.01, °°° p < 0.001: siGADD45A/siMYC vs. scr cells, overTOP2A vs. em cells. ( B ) Release of LDH, an index of cell necrosis, measured spectrophotometrically, in duplicates. Data are means ± SD ( n = 3). *** p < 0.001: Dox-treated U-2OS cells vs. untreated (-Dox) cells. ( C ) Representative photographs of crystal violet staining, used as assay of cell viability. The photograph is representative of one out of four experiments, in quadruplicates.
Blower Model Jabsco 35440, supplied by Jabsco GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/blower model jabsco-35440/product/Jabsco GmbH
Average 90 stars, based on 1 article reviews
blower model jabsco-35440 - by Bioz Stars, 2026-02
90/100 stars
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ucb-35440-3  (UCB SA Inc)
90
UCB SA Inc ucb-35440-3
GADD45A , MYC and TOP2A are responsible for the doxorubicin resistance induced by SNORD3A , SNORA13 and SNORA28 . U-2OS cells were transfected with an expression vector for SNORD3A , SNORA13 or SNORA28 . When indicated, cells were transiently transfected with a non-targeting scrambled <t>siRNA</t> pool (scr), with a GADD45A - or MYC -targeting siRNA (siGADD45A/siMYC) pool, with an empty vector (em) or with an expression vector for TOP2A (overTOP2A). U-2OS/DX30 and U-2OS/DX100 cells were included as Dox-resistant cells. Cells were treated with 5 μM Dox for 24 h (panel B ) or 72 h (panel C ). ( A ) mRNA levels of GADD45A , MYC and TOP2 A, evaluated by RT-PCR, in triplicates. Data are means ± SD ( n = 3). ** p < 0.01, *** p < 0.001: U-2OS/DX30, U-2OS/DX100 or snoRNA-over-expressing U-2OS cells vs. U-2OS cells; °° p < 0.01, °°° p < 0.001: siGADD45A/siMYC vs. scr cells, overTOP2A vs. em cells. ( B ) Release of LDH, an index of cell necrosis, measured spectrophotometrically, in duplicates. Data are means ± SD ( n = 3). *** p < 0.001: Dox-treated U-2OS cells vs. untreated (-Dox) cells. ( C ) Representative photographs of crystal violet staining, used as assay of cell viability. The photograph is representative of one out of four experiments, in quadruplicates.
Ucb 35440 3, supplied by UCB SA Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ucb-35440-3/product/UCB SA Inc
Average 90 stars, based on 1 article reviews
ucb-35440-3 - by Bioz Stars, 2026-02
90/100 stars
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Image Search Results


GADD45A , MYC and TOP2A are responsible for the doxorubicin resistance induced by SNORD3A , SNORA13 and SNORA28 . U-2OS cells were transfected with an expression vector for SNORD3A , SNORA13 or SNORA28 . When indicated, cells were transiently transfected with a non-targeting scrambled siRNA pool (scr), with a GADD45A - or MYC -targeting siRNA (siGADD45A/siMYC) pool, with an empty vector (em) or with an expression vector for TOP2A (overTOP2A). U-2OS/DX30 and U-2OS/DX100 cells were included as Dox-resistant cells. Cells were treated with 5 μM Dox for 24 h (panel B ) or 72 h (panel C ). ( A ) mRNA levels of GADD45A , MYC and TOP2 A, evaluated by RT-PCR, in triplicates. Data are means ± SD ( n = 3). ** p < 0.01, *** p < 0.001: U-2OS/DX30, U-2OS/DX100 or snoRNA-over-expressing U-2OS cells vs. U-2OS cells; °° p < 0.01, °°° p < 0.001: siGADD45A/siMYC vs. scr cells, overTOP2A vs. em cells. ( B ) Release of LDH, an index of cell necrosis, measured spectrophotometrically, in duplicates. Data are means ± SD ( n = 3). *** p < 0.001: Dox-treated U-2OS cells vs. untreated (-Dox) cells. ( C ) Representative photographs of crystal violet staining, used as assay of cell viability. The photograph is representative of one out of four experiments, in quadruplicates.

Journal: International Journal of Molecular Sciences

Article Title: Small Nucleolar RNAs Determine Resistance to Doxorubicin in Human Osteosarcoma

doi: 10.3390/ijms21124500

Figure Lengend Snippet: GADD45A , MYC and TOP2A are responsible for the doxorubicin resistance induced by SNORD3A , SNORA13 and SNORA28 . U-2OS cells were transfected with an expression vector for SNORD3A , SNORA13 or SNORA28 . When indicated, cells were transiently transfected with a non-targeting scrambled siRNA pool (scr), with a GADD45A - or MYC -targeting siRNA (siGADD45A/siMYC) pool, with an empty vector (em) or with an expression vector for TOP2A (overTOP2A). U-2OS/DX30 and U-2OS/DX100 cells were included as Dox-resistant cells. Cells were treated with 5 μM Dox for 24 h (panel B ) or 72 h (panel C ). ( A ) mRNA levels of GADD45A , MYC and TOP2 A, evaluated by RT-PCR, in triplicates. Data are means ± SD ( n = 3). ** p < 0.01, *** p < 0.001: U-2OS/DX30, U-2OS/DX100 or snoRNA-over-expressing U-2OS cells vs. U-2OS cells; °° p < 0.01, °°° p < 0.001: siGADD45A/siMYC vs. scr cells, overTOP2A vs. em cells. ( B ) Release of LDH, an index of cell necrosis, measured spectrophotometrically, in duplicates. Data are means ± SD ( n = 3). *** p < 0.001: Dox-treated U-2OS cells vs. untreated (-Dox) cells. ( C ) Representative photographs of crystal violet staining, used as assay of cell viability. The photograph is representative of one out of four experiments, in quadruplicates.

Article Snippet: In silencing experiments, cells were transfected with 0.5 μg of Control non-targeting siRNA plasmid (sc-37007); GADD 45α siRNA plasmid (sc-35440); c-Myc siRNA plasmid (sc-29226) (all from Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA).

Techniques: Transfection, Expressing, Plasmid Preparation, Reverse Transcription Polymerase Chain Reaction, Staining