35388 Search Results


sirna against mouse c flip s l  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology sirna against mouse c flip s l
FIG. 4. Analysis of Cflar (L) mRNA and protein levels and apoptotic levels in response to JO2 after knockdown of CFLAR (L) by <t>siRNA.</t> A) Representative gels for Cflar (L) mRNA levels from GC-2 cells at the nonpermissive TRP53 temperature exposed to Cflar siRNA for 24, 30, and 48 h. Actb is used as an internal control. C, Controls; CS, control siRNA transfected cells; F, Cflar siRNA transfected cells. B) Representative Western blots are provided; bands for CFLAR (L) (;55 kDa) and actB (;43 kDa) are shown here for cells grown at the nonpermissive TRP53 temperature, transfected with control siRNA (CS), Cflar siRNA (F), or matched time controls (C). C) Flow cytometry: a graphical representation of the percentage of GC-2 cells with or without Cflar siRNA transfection and exposed to JO2 (5 lg/ml) and undergoing apoptosis, or their matched time-point controls. Values represent the mean 6 SEM. Asterisk denotes significant differences (P , 0.05) in the apoptotic index between control Cflar siRNA transfected cells and siRNA transfected cells treated with JO2 at matched time points.
Sirna Against Mouse C Flip S L, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sirna against mouse c flip s l - by Bioz Stars, 2026-03
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human sox2 gene  (Addgene inc)
90
Addgene inc human sox2 gene
FIG. 4. Analysis of Cflar (L) mRNA and protein levels and apoptotic levels in response to JO2 after knockdown of CFLAR (L) by <t>siRNA.</t> A) Representative gels for Cflar (L) mRNA levels from GC-2 cells at the nonpermissive TRP53 temperature exposed to Cflar siRNA for 24, 30, and 48 h. Actb is used as an internal control. C, Controls; CS, control siRNA transfected cells; F, Cflar siRNA transfected cells. B) Representative Western blots are provided; bands for CFLAR (L) (;55 kDa) and actB (;43 kDa) are shown here for cells grown at the nonpermissive TRP53 temperature, transfected with control siRNA (CS), Cflar siRNA (F), or matched time controls (C). C) Flow cytometry: a graphical representation of the percentage of GC-2 cells with or without Cflar siRNA transfection and exposed to JO2 (5 lg/ml) and undergoing apoptosis, or their matched time-point controls. Values represent the mean 6 SEM. Asterisk denotes significant differences (P , 0.05) in the apoptotic index between control Cflar siRNA transfected cells and siRNA transfected cells treated with JO2 at matched time points.
Human Sox2 Gene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human sox2 gene/product/Addgene inc
Average 90 stars, based on 1 article reviews
human sox2 gene - by Bioz Stars, 2026-03
90/100 stars
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Image Search Results


FIG. 4. Analysis of Cflar (L) mRNA and protein levels and apoptotic levels in response to JO2 after knockdown of CFLAR (L) by siRNA. A) Representative gels for Cflar (L) mRNA levels from GC-2 cells at the nonpermissive TRP53 temperature exposed to Cflar siRNA for 24, 30, and 48 h. Actb is used as an internal control. C, Controls; CS, control siRNA transfected cells; F, Cflar siRNA transfected cells. B) Representative Western blots are provided; bands for CFLAR (L) (;55 kDa) and actB (;43 kDa) are shown here for cells grown at the nonpermissive TRP53 temperature, transfected with control siRNA (CS), Cflar siRNA (F), or matched time controls (C). C) Flow cytometry: a graphical representation of the percentage of GC-2 cells with or without Cflar siRNA transfection and exposed to JO2 (5 lg/ml) and undergoing apoptosis, or their matched time-point controls. Values represent the mean 6 SEM. Asterisk denotes significant differences (P , 0.05) in the apoptotic index between control Cflar siRNA transfected cells and siRNA transfected cells treated with JO2 at matched time points.

Journal: Biology of reproduction

Article Title: Influence of TRP53 status on FAS membrane localization, CFLAR (c-FLIP) ubiquitinylation, and sensitivity of GC-2spd (ts) cells to undergo FAS-mediated apoptosis.

doi: 10.1095/biolreprod.105.045146

Figure Lengend Snippet: FIG. 4. Analysis of Cflar (L) mRNA and protein levels and apoptotic levels in response to JO2 after knockdown of CFLAR (L) by siRNA. A) Representative gels for Cflar (L) mRNA levels from GC-2 cells at the nonpermissive TRP53 temperature exposed to Cflar siRNA for 24, 30, and 48 h. Actb is used as an internal control. C, Controls; CS, control siRNA transfected cells; F, Cflar siRNA transfected cells. B) Representative Western blots are provided; bands for CFLAR (L) (;55 kDa) and actB (;43 kDa) are shown here for cells grown at the nonpermissive TRP53 temperature, transfected with control siRNA (CS), Cflar siRNA (F), or matched time controls (C). C) Flow cytometry: a graphical representation of the percentage of GC-2 cells with or without Cflar siRNA transfection and exposed to JO2 (5 lg/ml) and undergoing apoptosis, or their matched time-point controls. Values represent the mean 6 SEM. Asterisk denotes significant differences (P , 0.05) in the apoptotic index between control Cflar siRNA transfected cells and siRNA transfected cells treated with JO2 at matched time points.

Article Snippet: We obtained siRNA against mouse c-FLIP (S/L) from Santa Cruz Biotechnology (sc-35389) to knock down the protein expression of CFLAR (L) in GC-2 cells.

Techniques: Knockdown, Control, Transfection, Western Blot, Flow Cytometry