35097 Search Results


methanothermococcus thermolithotrophicus atcc 35097  (ATCC)
90
ATCC methanothermococcus thermolithotrophicus atcc 35097
Methanothermococcus Thermolithotrophicus Atcc 35097, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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methanothermococcus thermolithotrophicus atcc 35097 - by Bioz Stars, 2026-01
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neuregulin 1  (R&D Systems)
92
R&D Systems neuregulin 1
Neuregulin 1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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neuregulin 1 - by Bioz Stars, 2026-01
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exrai ampkar  (Addgene inc)
94
Addgene inc exrai ampkar
Exrai Ampkar, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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exrai ampkar - by Bioz Stars, 2026-01
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cox1 gene  (Santa Cruz Biotechnology)
92
Santa Cruz Biotechnology cox1 gene
Cox1 Gene, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cox1 shrna plasmid  (Santa Cruz Biotechnology)
92
Santa Cruz Biotechnology cox1 shrna plasmid
FIGURE 3 Characterization of C18-4Cox1-KD1 cells and KD-associated morphological and gene changes. (A) Expression of cyclooxygenase (Cox)1 on populations of transfected cells with varying concentrations of <t>shRNA</t> and lipofectamine 3000 showing that knockdown “B” had the most downregulated expression of <t>Cox1.</t> (B) Cox1 protein expression on immunoblot, comparing wildtype cells (WT), scrambled cells (Scrb), knockdown “B” clone, and an isolated clonal population of C18-4Cox1-KD1. C18-4Cox1-KD1 Cox1 protein expression validated against the total protein. (C) Gene expression of Cox1 in C18-4Cox1-KD1 and Scrambled controls, (D) Immunofluorescence staining of Cox1 in C18-4Cox1-KD1 and Scrambled controls. Green: Cox1, red: alpha-tubulin, blue: DAPI. Scale in µm. (E) Brightfield visualization illustrating morphological differences between C18-4Cox1-KD1 and Scrambled controls. (F) Differences in gene expression between C18-4Cox1-KD1 and Scrambled controls of Jam-1, Mmp2, Stra8, and Kit. Results are presented as fold change of controls. mRNA expression data were normalized to Glyceraldehyde 3-phosphate dehydrogenase (Gapdh); N = 3 independent experiments conducted in triplicates. Significant difference relative to controls with t-test: * (p ≤0.05), ** (p < 0.01), *** (p < 0.001).
Cox1 Shrna Plasmid, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


FIGURE 3 Characterization of C18-4Cox1-KD1 cells and KD-associated morphological and gene changes. (A) Expression of cyclooxygenase (Cox)1 on populations of transfected cells with varying concentrations of shRNA and lipofectamine 3000 showing that knockdown “B” had the most downregulated expression of Cox1. (B) Cox1 protein expression on immunoblot, comparing wildtype cells (WT), scrambled cells (Scrb), knockdown “B” clone, and an isolated clonal population of C18-4Cox1-KD1. C18-4Cox1-KD1 Cox1 protein expression validated against the total protein. (C) Gene expression of Cox1 in C18-4Cox1-KD1 and Scrambled controls, (D) Immunofluorescence staining of Cox1 in C18-4Cox1-KD1 and Scrambled controls. Green: Cox1, red: alpha-tubulin, blue: DAPI. Scale in µm. (E) Brightfield visualization illustrating morphological differences between C18-4Cox1-KD1 and Scrambled controls. (F) Differences in gene expression between C18-4Cox1-KD1 and Scrambled controls of Jam-1, Mmp2, Stra8, and Kit. Results are presented as fold change of controls. mRNA expression data were normalized to Glyceraldehyde 3-phosphate dehydrogenase (Gapdh); N = 3 independent experiments conducted in triplicates. Significant difference relative to controls with t-test: * (p ≤0.05), ** (p < 0.01), *** (p < 0.001).

Journal: Andrology

Article Title: Differential roles of cyclooxygenase enzymes in the regulation of murine juvenile undifferentiated spermatogonia.

doi: 10.1111/andr.13537

Figure Lengend Snippet: FIGURE 3 Characterization of C18-4Cox1-KD1 cells and KD-associated morphological and gene changes. (A) Expression of cyclooxygenase (Cox)1 on populations of transfected cells with varying concentrations of shRNA and lipofectamine 3000 showing that knockdown “B” had the most downregulated expression of Cox1. (B) Cox1 protein expression on immunoblot, comparing wildtype cells (WT), scrambled cells (Scrb), knockdown “B” clone, and an isolated clonal population of C18-4Cox1-KD1. C18-4Cox1-KD1 Cox1 protein expression validated against the total protein. (C) Gene expression of Cox1 in C18-4Cox1-KD1 and Scrambled controls, (D) Immunofluorescence staining of Cox1 in C18-4Cox1-KD1 and Scrambled controls. Green: Cox1, red: alpha-tubulin, blue: DAPI. Scale in µm. (E) Brightfield visualization illustrating morphological differences between C18-4Cox1-KD1 and Scrambled controls. (F) Differences in gene expression between C18-4Cox1-KD1 and Scrambled controls of Jam-1, Mmp2, Stra8, and Kit. Results are presented as fold change of controls. mRNA expression data were normalized to Glyceraldehyde 3-phosphate dehydrogenase (Gapdh); N = 3 independent experiments conducted in triplicates. Significant difference relative to controls with t-test: * (p ≤0.05), ** (p < 0.01), *** (p < 0.001).

Article Snippet: Cox1 shRNA plasmid for a mouse (sc-35097-SH) and control shRNA plasmid (sc-108060) were purchased from Santa Cruz Biotech.

Techniques: Expressing, Transfection, shRNA, Knockdown, Western Blot, Isolation, Gene Expression, Immunofluorescence, Staining

FIGURE 5 Activation of Notch3 signaling pathway in C18-4Cox1-KD1. (A) Immunofluorescence (IF) images of Notch3 expression in C18-4Cox1-KD1 and Scrambled controls. Green: Notch3, red: alpha-tubulin, blue: DAPI. Scale in µm. (B) Immunoblots of Notch3, Hes1, cyclooxygenase (Cox)1, and Cox2 expression in C18-4Cox1-KD1 and Scrambled controls are cropped at indicated bands, and protein expression, indicated by values under the bands, is normalized to alpha-tubulin. (C) Quantification of Notch3, Hes1, Cox1, and Cox2 protein expression on western blot normalized against the total protein. Results are presented as fold change of controls. (D) IF images of Hes1 expression in C18-4Cox1-KD1 and Scrambled controls. Green: Hes1, red: alpha-tubulin, blue: DAPI. Hes1-positive cells were counted and the data show the percentage of Hes-positive cells as fold of control samples. N = 3 independent experiments. Significant difference relative to controls with t-test: * (p ≤0.05), ** (p < 0.01), *** (p < 0.001).

Journal: Andrology

Article Title: Differential roles of cyclooxygenase enzymes in the regulation of murine juvenile undifferentiated spermatogonia.

doi: 10.1111/andr.13537

Figure Lengend Snippet: FIGURE 5 Activation of Notch3 signaling pathway in C18-4Cox1-KD1. (A) Immunofluorescence (IF) images of Notch3 expression in C18-4Cox1-KD1 and Scrambled controls. Green: Notch3, red: alpha-tubulin, blue: DAPI. Scale in µm. (B) Immunoblots of Notch3, Hes1, cyclooxygenase (Cox)1, and Cox2 expression in C18-4Cox1-KD1 and Scrambled controls are cropped at indicated bands, and protein expression, indicated by values under the bands, is normalized to alpha-tubulin. (C) Quantification of Notch3, Hes1, Cox1, and Cox2 protein expression on western blot normalized against the total protein. Results are presented as fold change of controls. (D) IF images of Hes1 expression in C18-4Cox1-KD1 and Scrambled controls. Green: Hes1, red: alpha-tubulin, blue: DAPI. Hes1-positive cells were counted and the data show the percentage of Hes-positive cells as fold of control samples. N = 3 independent experiments. Significant difference relative to controls with t-test: * (p ≤0.05), ** (p < 0.01), *** (p < 0.001).

Article Snippet: Cox1 shRNA plasmid for a mouse (sc-35097-SH) and control shRNA plasmid (sc-108060) were purchased from Santa Cruz Biotech.

Techniques: Activation Assay, Immunofluorescence, Expressing, Western Blot, Control