Journal: PLoS ONE

Article Title: Nanotube Action between Human Mesothelial Cells Reveals Novel Aspects of Inflammatory Responses

doi: 10.1371/journal.pone.0029537

Figure Lengend Snippet: (A, B) Influence of TNF-α on actin cytoskeleton reorganization in HPMCs. ( A ) The fluorescence picture shows a predominant localization of F-actin at the rim of the cells (arrows) in absence of TNF-α. ( B ) The presence of TNF-α leads to reorganization of the actin cytoskeleton accompanied by the formation of actin stress fibers (arrowheads). Scale bars: 50 µm. ( C , D ) Spatial separation of HPMCs results in complete inhibition of NT formation. ( C ) Scanning electron and live-cell fluorescence microscopy pictures of HPMCs one hour after plating on homogeneously collagen I coated glass cover slides. The white arrows point to NTs spanned between the cells. Scale bars (left): 100 µm; (right): 20 µm. ( D ) HPMCs seeded on IKVAV functionalized (50 µM) microstructured surfaces with a gold disk diameter and spacing of 50 µm. Scanning electron microscopy (left panel) and live-cell imaging analyses (right panel) were performed one hour after plating. Note that cells exclusively attach to the biofunctionalized gold disk structures. The inserts in A, B and D represent higher magnified images. Scale bars (left): 100 µm; (right): 20 µm.

Article Snippet: To couple IKVAV peptide (Bachem) covalently on the gold discs, surfaces were incubated for at least 12 hours at 4°C in a 50 µM IKVAV solution in PB buffer.

Techniques: Fluorescence, Inhibition, Microscopy, Electron Microscopy, Live Cell Imaging

Journal: Science Advances

Article Title: Bionic microenvironment-inspired synergistic effect of anisotropic micro-nanocomposite topology and biology cues on peripheral nerve regeneration

doi: 10.1126/sciadv.abi5812

Figure Lengend Snippet: ( A ) FTIR analysis. (a) Pristine PCL, (b) PCL/dopamine (Dopa), and (c) PCL/Dopa/IKVAV. ( B ) Amino density using AO II test, * P < 0.05. ( C ) Density of IKVAV peptide, * P < 0.05, PCL versus PCL/Dopa; ** P < 0.05, PCL/Dopa versus PCL/Dopa/IKVAV; and *** P < 0.05, PCL versus PCL/Dopa/IKVAV, ( D ) SEM observation of Dopa and IKVAV on PCL fibers. ( E ) Release behavior of IKVAV from 0 min to 72 hours. ( F ) Wettability as a function of Dopa and IKVAV treatment, * P < 0.05, compared with other groups. ( G ) Immunofluorescence staining of IKVAV in different samples; seven locations of each sample were randomly captured under the same magnification for systematical analysis.

Article Snippet: IKVAV peptide and FITC-labeled IKVAV peptide were purchased from GL Biochem, China.

Techniques: Immunofluorescence, Staining

Journal: Science Advances

Article Title: Bionic microenvironment-inspired synergistic effect of anisotropic micro-nanocomposite topology and biology cues on peripheral nerve regeneration

doi: 10.1126/sciadv.abi5812

Figure Lengend Snippet: ( A ) Morphology observation using TBO staining (scale bar, 30 μm), immunofluorescence staining (scale bar, 50 μm), and SEM (scale bar, 20 μm); the yellow arrows indicate the cytoskeleton of Schwann cells. ( B ) Number. * P < 0.05, R/IKVAV versus A/IKVAV and M/IKVAV groups and ** P < 0.05, M/A/IKVAV versus A/IKVAV and M/IKVAV groups. ( C ) Orientation angle, ( D ) area, and ( E ) ratio of L/W of Schwann cells on different samples; * P < 0.05, R/IKVAV versus other groups. (a) R/IKVAV, (b) A/IKVAV, (c) M/IKVAV, and (d) M/A/IKVAV.

Article Snippet: IKVAV peptide and FITC-labeled IKVAV peptide were purchased from GL Biochem, China.

Techniques: Staining, Immunofluorescence

Journal: Science Advances

Article Title: Bionic microenvironment-inspired synergistic effect of anisotropic micro-nanocomposite topology and biology cues on peripheral nerve regeneration

doi: 10.1126/sciadv.abi5812

Figure Lengend Snippet: ( A ) Transcriptomic sequence. (top) Up-/down-regulated genes in Schwann cells, (bottom) heatmap of top differentially expressed genes significantly altered in different groups. ( B ) Gene expression by qRT-PCR test, * P < 0.05, compared with other groups and ** P < 0.05, A/IKVAV versus M/IKVAV and M/A/IKVAV groups. ( C ) Protein expression by Western blot assay. * P < 0.05 versus other groups; ** P < 0.05, R/IKVAV versus A/IKVAV; and *** P < 0.05, A/IKVAV versus M/IKVAV. (a) R/IKVAV, (b) A/IKVAV, (c) M/IKVAV, and (d) M/A/IKVAV.

Article Snippet: IKVAV peptide and FITC-labeled IKVAV peptide were purchased from GL Biochem, China.

Techniques: Sequencing, Expressing, Quantitative RT-PCR, Western Blot

Journal: Science Advances

Article Title: Bionic microenvironment-inspired synergistic effect of anisotropic micro-nanocomposite topology and biology cues on peripheral nerve regeneration

doi: 10.1126/sciadv.abi5812

Figure Lengend Snippet: ( A ) Implantation of samples (black arrows) in chicken embryos. ( B ) Tissue transparency analysis under bright-field (BF) and immunofluorescence (IF) staining using CD31 antibody for analyzing newborn blood vessels, ( C ) total length, ( D ) branches, [(C and D) * P < 0.05, R/IKVAV and A/IKVAV versus M/IKVAV and ** P < 0.05 M/IKVAV versus M/A/IKVAV], ( E ) diameter distribution, and ( F ) aligned degree of blood vessels in different samples [(E and F) * P < 0.05, M/IKVAV versus other groups].

Article Snippet: IKVAV peptide and FITC-labeled IKVAV peptide were purchased from GL Biochem, China.

Techniques: Immunofluorescence, Staining

Journal: BMC Cell Biology

Article Title: Cell adhesion to agrin presented as a nanopatterned substrate is consistent with an interaction with the extracellular matrix and not transmembrane adhesion molecules

doi: 10.1186/1471-2121-9-64

Figure Lengend Snippet: Peptide inhibition assay on 60 nm spaced C100 Agrin substrates. The IKVAV peptide reduces B35 cell adhesion to approximately 50% of the control (no peptide included). RGD and HAV do not significantly affect cell adhesion.

Article Snippet: For adhesion assays with peptide-inhibition, cells were incubated with 100 μg/ml RGD peptide (Sigma), 100 μg/ml IKVAV peptide (Sigma), or 1 mg/ml HAV peptide (American Peptide).

Techniques: Peptide Inhibition Assay