Journal: mSystems

Article Title: Integrated Multi-omics Investigations Reveal the Key Role of Synergistic Microbial Networks in Removing Plasticizer Di-(2-Ethylhexyl) Phthalate from Estuarine Sediments

doi: 10.1128/mSystems.00358-21

Figure Lengend Snippet: Hydrolases potentially involved in the degradation of DEHP alkyl side chain in DEHP-treated sediments

Article Snippet: The unrooted maximum likelihood tree of phthaloyl-CoA decarboxylase showed that eight genes annotated as phthaloyl-CoA decarboxylase in the gammaproteobacterial bins (except for k141_3428055_1, k141_2680687_1, and k141_1830739_7) formed a distinct clade, whereas another phthaloyl-CoA decarboxylase gene (k141_1104744_1) recovered in Azoarcus Bin394 was placed in the same clade with phthaloyl-CoA decarboxylase genes derived from denitrifying betaproteobacteria ( A . aromaticum DSM 19081, A. toluclasticus ATCC 700605, T . chlorobenzoica DSM 18012, and Azoarcus sp. PA01).

Techniques:

Journal: mSystems

Article Title: Integrated Multi-omics Investigations Reveal the Key Role of Synergistic Microbial Networks in Removing Plasticizer Di-(2-Ethylhexyl) Phthalate from Estuarine Sediments

doi: 10.1128/mSystems.00358-21

Figure Lengend Snippet: Maximum likelihood tree of phthaloyl-CoA decarboxylase from bacterial isolates and in DEHP-treated sediments. Orange branch, gammaproteobacterial nitrate-reducing phthalic acid degraders; blue branch, betaproteobacterial nitrate-reducing o -phthalic acid degraders; green branch, deltaproteobacterial sulfate-reducing phthalic acid degraders. Branch support of higher than 50% of the bootstrapping time is shown.

Article Snippet: The unrooted maximum likelihood tree of phthaloyl-CoA decarboxylase showed that eight genes annotated as phthaloyl-CoA decarboxylase in the gammaproteobacterial bins (except for k141_3428055_1, k141_2680687_1, and k141_1830739_7) formed a distinct clade, whereas another phthaloyl-CoA decarboxylase gene (k141_1104744_1) recovered in Azoarcus Bin394 was placed in the same clade with phthaloyl-CoA decarboxylase genes derived from denitrifying betaproteobacteria ( A . aromaticum DSM 19081, A. toluclasticus ATCC 700605, T . chlorobenzoica DSM 18012, and Azoarcus sp. PA01).

Techniques:

Journal: mSystems

Article Title: Integrated Multi-omics Investigations Reveal the Key Role of Synergistic Microbial Networks in Removing Plasticizer Di-(2-Ethylhexyl) Phthalate from Estuarine Sediments

doi: 10.1128/mSystems.00358-21

Figure Lengend Snippet: Maximum likelihood tree of hydrolases involved in degradation of alkyl side chains on DEHP and other PAEs and other hydrolases from Acidovorax sp. strain 210-6. The phylogeny of the protein sequences from NCBI and UniProt with an identity ≥40% similar to NCU65467 of Acidovorax sp. strain 210-6 was also inferred. Purple branch, aerobic alphaproteobacterial degraders; blue branch, anaerobic betaproteobacterial degraders; red branch, aerobic actinobacterial degraders; orange branch, anaerobic gammaproteobacterial degraders; brown branch, aerobic Firmicutes degraders. Branch support of more than 50% of the bootstrapping time is shown.

Article Snippet: The unrooted maximum likelihood tree of phthaloyl-CoA decarboxylase showed that eight genes annotated as phthaloyl-CoA decarboxylase in the gammaproteobacterial bins (except for k141_3428055_1, k141_2680687_1, and k141_1830739_7) formed a distinct clade, whereas another phthaloyl-CoA decarboxylase gene (k141_1104744_1) recovered in Azoarcus Bin394 was placed in the same clade with phthaloyl-CoA decarboxylase genes derived from denitrifying betaproteobacteria ( A . aromaticum DSM 19081, A. toluclasticus ATCC 700605, T . chlorobenzoica DSM 18012, and Azoarcus sp. PA01).

Techniques:

Journal: mSystems

Article Title: Integrated Multi-omics Investigations Reveal the Key Role of Synergistic Microbial Networks in Removing Plasticizer Di-(2-Ethylhexyl) Phthalate from Estuarine Sediments

doi: 10.1128/mSystems.00358-21

Figure Lengend Snippet: Diagram of proposed DEHP degradation through microbial synergistic metabolism in denitrifying sediments. The betaproteobacterium Acidovorax and gammaproteobacterium Sedimenticola are major degraders of DEHP side chains. The resulting o -phthalic acid is then imported through a specific TRAP transporter and degraded by other betaproteobacteria (e.g., Azoarcus ) and gammaproteobacteria (e.g., Aestuariibacter ). *, Gammaproteobacterial Bin44 has degradation capacity for both side chains and o -phthalic acid. Orange, Gammaproteobacteria ; blue, Betaproteobacteria .

Article Snippet: The unrooted maximum likelihood tree of phthaloyl-CoA decarboxylase showed that eight genes annotated as phthaloyl-CoA decarboxylase in the gammaproteobacterial bins (except for k141_3428055_1, k141_2680687_1, and k141_1830739_7) formed a distinct clade, whereas another phthaloyl-CoA decarboxylase gene (k141_1104744_1) recovered in Azoarcus Bin394 was placed in the same clade with phthaloyl-CoA decarboxylase genes derived from denitrifying betaproteobacteria ( A . aromaticum DSM 19081, A. toluclasticus ATCC 700605, T . chlorobenzoica DSM 18012, and Azoarcus sp. PA01).

Techniques: