33569 Search Results


90
ATCC atcc 33569
Atcc 33569, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
atcc 33569 - by Bioz Stars, 2026-03
90/100 stars
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86
Biosynth Carbosynth sheep anti egfr antibody
Fig. 6. Immuno-EM localization of wt <t>EGFR</t> and Y1045F EGFR in cells incubated with EGF. Thawed cryosections of cells expressing either wt EGFR (A) or Y1045F EGFR (B–D) were labeled using <t>anti</t> <t>EGFR</t> antibodies (Ab-3 and sc-120) and 15 nm protein A gold. The cells had been incubated with 108 M EGF for 10 min at 378C and either fixed immediately (A and B) or after 60 min chase in EGF-free medium (C and D). Based on morphology, two EGFR-positive compartments were identified, early endosomes (e.e) and MVBs. Note that although labeling for Y1045F EGFR could be found in MVBs (C), the majority of intracellular Y1045F EGFR labeling localized to early endosomes even upon 60 min chase (D). Scale bars, 100 nm.
Sheep Anti Egfr Antibody, supplied by Biosynth Carbosynth, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sheep anti egfr antibody/product/Biosynth Carbosynth
Average 86 stars, based on 1 article reviews
sheep anti egfr antibody - by Bioz Stars, 2026-03
86/100 stars
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Image Search Results


Fig. 6. Immuno-EM localization of wt EGFR and Y1045F EGFR in cells incubated with EGF. Thawed cryosections of cells expressing either wt EGFR (A) or Y1045F EGFR (B–D) were labeled using anti EGFR antibodies (Ab-3 and sc-120) and 15 nm protein A gold. The cells had been incubated with 108 M EGF for 10 min at 378C and either fixed immediately (A and B) or after 60 min chase in EGF-free medium (C and D). Based on morphology, two EGFR-positive compartments were identified, early endosomes (e.e) and MVBs. Note that although labeling for Y1045F EGFR could be found in MVBs (C), the majority of intracellular Y1045F EGFR labeling localized to early endosomes even upon 60 min chase (D). Scale bars, 100 nm.

Journal: Experimental cell research

Article Title: Direct interaction of Cbl with pTyr 1045 of the EGF receptor (EGFR) is required to sort the EGFR to lysosomes for degradation.

doi: 10.1016/j.yexcr.2004.07.003

Figure Lengend Snippet: Fig. 6. Immuno-EM localization of wt EGFR and Y1045F EGFR in cells incubated with EGF. Thawed cryosections of cells expressing either wt EGFR (A) or Y1045F EGFR (B–D) were labeled using anti EGFR antibodies (Ab-3 and sc-120) and 15 nm protein A gold. The cells had been incubated with 108 M EGF for 10 min at 378C and either fixed immediately (A and B) or after 60 min chase in EGF-free medium (C and D). Based on morphology, two EGFR-positive compartments were identified, early endosomes (e.e) and MVBs. Note that although labeling for Y1045F EGFR could be found in MVBs (C), the majority of intracellular Y1045F EGFR labeling localized to early endosomes even upon 60 min chase (D). Scale bars, 100 nm.

Article Snippet: Mouse anti-phosphorylated EGFR (pY1173) antibody was from Upstate (Lake Placid, NY), and sheep anti-EGFR antibody was from Fitzgerald (Concord, MA).

Techniques: Incubation, Expressing, Labeling

Fig. 8. Colocalization of wt EGFR and Y1045F EGFR with Hrs. Thawed cryosections of cells expressing either wt EGFR (A) or Y1045F EGFR (B) incubated with 108 M EGF for 5 min at 378C were double labeled using anti Hrs antibodies (small gold particles) followed by anti EGFR antibodies (large gold particles). Arrowheads indicate the Hrs-positive coat on the limiting membrane of early endosomes. Scale bar, 100 nm.

Journal: Experimental cell research

Article Title: Direct interaction of Cbl with pTyr 1045 of the EGF receptor (EGFR) is required to sort the EGFR to lysosomes for degradation.

doi: 10.1016/j.yexcr.2004.07.003

Figure Lengend Snippet: Fig. 8. Colocalization of wt EGFR and Y1045F EGFR with Hrs. Thawed cryosections of cells expressing either wt EGFR (A) or Y1045F EGFR (B) incubated with 108 M EGF for 5 min at 378C were double labeled using anti Hrs antibodies (small gold particles) followed by anti EGFR antibodies (large gold particles). Arrowheads indicate the Hrs-positive coat on the limiting membrane of early endosomes. Scale bar, 100 nm.

Article Snippet: Mouse anti-phosphorylated EGFR (pY1173) antibody was from Upstate (Lake Placid, NY), and sheep anti-EGFR antibody was from Fitzgerald (Concord, MA).

Techniques: Expressing, Incubation, Labeling, Membrane