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Image Search Results
Journal: International journal of molecular medicine
Article Title: Nrf2 activation diminishes during adipocyte differentiation of ST2 cells.
doi: 10.3892/ijmm.2011.761
Figure Lengend Snippet: Figure 1. The intracellular glutathione redox potential becomes more oxidized during adipocyte differentiation of ST2 cells. ST2 cells stably expressing Grx1- roGFP2 were induced to differentiate into adipocytes. Every 24 h the cells were excited with 390 and 480 nm lasers and the ratio of emissions in the green channel (535 nm) was calculated. The graph represents the mean ± SD. Three independent experiments were performed, and in each experiment 24 technical replicates were used for each timepoint. *p<0.0001 compared to day 0; †p<0.001; ††p<0.0001.
Article Snippet:
Techniques: Stable Transfection, Expressing
Journal: International journal of molecular medicine
Article Title: Nrf2 activation diminishes during adipocyte differentiation of ST2 cells.
doi: 10.3892/ijmm.2011.761
Figure Lengend Snippet: Figure 2. Nuclear Nrf2 protein abundance decreases in ST2 cells during adi- pocyte differentiation. ST2 cells were induced to differentiate into adipocytes. Nuclear protein extracts were prepared immediately before the initiation of dif- ferentiation (day 0) and every 24 h thereafter until day 5. Nrf2 expression was assayed by Western immunoblotting. The nuclear abundance of the transcrip- tion factor SP-1 was employed as a loading control. The image is representative of 3 independent experiments. The graph represents the mean ± SD. *p<0.0001 compared to day 0. †p<0.05; ††p<0.0001.
Article Snippet:
Techniques: Quantitative Proteomics, Expressing, Western Blot, Control
Journal: International journal of molecular medicine
Article Title: Nrf2 activation diminishes during adipocyte differentiation of ST2 cells.
doi: 10.3892/ijmm.2011.761
Figure Lengend Snippet: Figure 3. Nrf2 binding activity decreases during adipocyte differentiation of ST2 cells. ST2 cells were induced to differentiate into adipocytes. Nuclear protein extracts were prepared before the initiation of differentiation (day 0) and every 24 h thereafter until day 5, and were used for EMSAs with an ARE- containing oligonucleotide as a radiolabelled probe. The image is representative of 3 independent experiments. Binding competition experiments performed with ST2 nuclear extracts at day 0 (0d) of differentiation (maximal Nrf2 DNA binding activity) in the presence of a 10-, 50-, or 100-fold molar excess of unlabeled ARE-wt or ARE-mut oligonucleotides. The image is representative of 3 independent experiments.
Article Snippet:
Techniques: Binding Assay, Activity Assay
Journal: International journal of molecular medicine
Article Title: Nrf2 activation diminishes during adipocyte differentiation of ST2 cells.
doi: 10.3892/ijmm.2011.761
Figure Lengend Snippet: Figure 4. ARE-mediated transcription is reduced during differentiation of ARE-ST2 cells into adipocytes. ARE-ST2 cells were induced to differentiate into adipocytes and luciferase measurements were performed every 24 h until day 5 of differentiation. Luciferase activity was normalized to total protein concentration. The graph represents the means ± SD. Three independent exper- iments were performed, and in each experiment 8 technical replicates were used for each timepoint. *p<0.0001 compared to day 0; †p<0.05; ††p<0.0001.
Article Snippet:
Techniques: Luciferase, Activity Assay, Protein Concentration