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ATCC
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ATCC
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Harrick Plasma Inc
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DUTSCHER DOMINIQUE
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Image Search Results
Journal: Oncotarget
Article Title: Mapping of Apoptin-interaction with BCR-ABL1, and development of apoptin-based targeted therapy
doi:
Figure Lengend Snippet: (a) Elevated level of cleaved PARP-1 in 32D p210 cells treated with apoptin. (b) Appearance of cleaved PARP-1 and induction of apoptosis in Bcr-Abl expressing 32D p210 cells when treated with apoptin or imatinib; (c) The effects of apoptin on the survival of Bcr-Abl expressing cells as determined by Nicoletti method. N=3. *P<0.03.
Article Snippet: As shown in figure apoptin derived decapeptide treatment does not show any significant cellular toxicity among
Techniques: Expressing
Journal: Oncotarget
Article Title: Mapping of Apoptin-interaction with BCR-ABL1, and development of apoptin-based targeted therapy
doi:
Figure Lengend Snippet: (a) . Schematic representation of apoptin deletion mutants tagged with N-terminal GFP. (b) . Immunoblot showing the expression of deletion mutants and wild type apoptin (1–121) transfected into PC-3 cells and immunoprecipitated with anti-GFP antibody 18 h post-transfection. (c) . Apoptin CO-IP experiment from transfected 32D DMSZ , 32D p210 and K562 cells with various mutant forms of apoptin; Bcr-Abl was identified in the immuno-precipitates of full-length apoptin and apoptin derivatives that harbored amino acids from 74-100 (includes the proline rich region, aa: 81-86) indicating a part of this region of apoptin is important for the interaction with Bcr-Abl p210 .
Article Snippet: As shown in figure apoptin derived decapeptide treatment does not show any significant cellular toxicity among
Techniques: Western Blot, Expressing, Transfection, Immunoprecipitation, Co-Immunoprecipitation Assay, Mutagenesis
Journal: Oncotarget
Article Title: Mapping of Apoptin-interaction with BCR-ABL1, and development of apoptin-based targeted therapy
doi:
Figure Lengend Snippet: (a) The effects of Tat-conjugated apoptin derived peptide on the survival of Bcr-Abl non-expressing 32D DSMZ cells (MTT assay). (b) the effects of Tat-conjugated apoptin derived peptide on the survival of Bcr-Abl expressing 32D p210 cells (MTT assay). (c) the effects of Tat-conjugated apoptin derived peptide on the survival of Bcr-Abl expressing K562 cells (MTT assay). (d, e, f) . Both Imatinib responsive and resistant patient samples are sensitive to apoptin derived decapeptide but not healthy donor samples. MTT assay results show a time dependent cell death by apoptin decapeptide at 16h, 36h and 48h. N=3. *p<0.05.
Article Snippet: As shown in figure apoptin derived decapeptide treatment does not show any significant cellular toxicity among
Techniques: Derivative Assay, Expressing, MTT Assay