32d Search Results


cell culture murine 32d  (ATCC)
94
ATCC cell culture murine 32d
Cell Culture Murine 32d, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/32d/pmc03972068-79-1-10?v=ATCC
Average 94 stars, based on 1 article reviews
cell culture murine 32d - by Bioz Stars, 2026-07
94/100 stars
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32d clone3 atcc crl 3594 software  (ATCC)
94
ATCC 32d clone3 atcc crl 3594 software
32d Clone3 Atcc Crl 3594 Software, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/32d/pm41709745-406-101-103?v=ATCC
Average 94 stars, based on 1 article reviews
32d clone3 atcc crl 3594 software - by Bioz Stars, 2026-07
94/100 stars
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32d  (DSMZ)
93
DSMZ 32d
OSM is induced by STAT5-activating oncogenes in a kinase-dependent manner and acts on bone marrow stromal cells via STAT3 phosphorylation. a <t>32D</t> cells were transduced with various oncogenes and starved of cytokines and serum for twelve hours. Quantitative PCR revealed Osm upregulation in BCR::ABL1-, FLT3-ITD-, NPM1::ALK-, and JAK2 p.V617F-positive cells. Osm expression values were normalized to Gapdh and empty vector (MiG-empty) expression values. N ≥ 3 experiments; p values (compared with MiG-empty cells): *** p < 0.001; ** p < 0.01. The data are presented as the means ± SEMs. b 32D cells transduced with oncogenes were serum-starved plus inhibitor-treated as indicated for twelve hours. Osm expression values were normalized to Gapdh and MiG-empty vector expression values. P values (compared with MiG-empty cells): *** p < 0.001; ** p < 0.01. The data are presented as the means ± SEMs. c Flow cytometric analysis of OSMR surface levels in primary human stromal cells, primary AML blasts, and human myeloid leukemic cell lines. The mean fluorescence intensity (MFI) of the isotype control was subtracted from the OSMR MFI values. Each dot represents one measurement. The data are presented as the means ± SEMs. d Primary murine hematopoietic BM cells (HCs) did not express Osmr transcripts after 40 PCR cycles, whereas mesenchymal stromal cell (MSC) populations expressed Osmr . MSC = mesenchymal stromal cells, OB = osteoblasts, ND = not detected. Each dot represents one measurement. The data are presented as the means ± SEMs. e Flow cytometric analysis of pSTAT3 levels in primary human stromal cells and human myeloid leukemic cell lines after huOSM treatment (2.5 h, 10 ng/mL). The mean fluorescence intensity (MFI) of the isotype control was subtracted from the pSTAT3 antibody MFI values. Each dot represents one measurement. The data are presented as the means ± SEMs. f , g OSM ( f ) and OSMR ( g ) expression across hematological and solid neoplasms utilizing Expression Public 24Q2 and lineage subtype grouping on the DepMap portal ( https://depmap.org )
32d, supplied by DSMZ, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/32d/pmc12696130-339-0-15?v=DSMZ
Average 93 stars, based on 1 article reviews
32d - by Bioz Stars, 2026-07
93/100 stars
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crl11346  (ATCC)
93
ATCC crl11346
OSM is induced by STAT5-activating oncogenes in a kinase-dependent manner and acts on bone marrow stromal cells via STAT3 phosphorylation. a <t>32D</t> cells were transduced with various oncogenes and starved of cytokines and serum for twelve hours. Quantitative PCR revealed Osm upregulation in BCR::ABL1-, FLT3-ITD-, NPM1::ALK-, and JAK2 p.V617F-positive cells. Osm expression values were normalized to Gapdh and empty vector (MiG-empty) expression values. N ≥ 3 experiments; p values (compared with MiG-empty cells): *** p < 0.001; ** p < 0.01. The data are presented as the means ± SEMs. b 32D cells transduced with oncogenes were serum-starved plus inhibitor-treated as indicated for twelve hours. Osm expression values were normalized to Gapdh and MiG-empty vector expression values. P values (compared with MiG-empty cells): *** p < 0.001; ** p < 0.01. The data are presented as the means ± SEMs. c Flow cytometric analysis of OSMR surface levels in primary human stromal cells, primary AML blasts, and human myeloid leukemic cell lines. The mean fluorescence intensity (MFI) of the isotype control was subtracted from the OSMR MFI values. Each dot represents one measurement. The data are presented as the means ± SEMs. d Primary murine hematopoietic BM cells (HCs) did not express Osmr transcripts after 40 PCR cycles, whereas mesenchymal stromal cell (MSC) populations expressed Osmr . MSC = mesenchymal stromal cells, OB = osteoblasts, ND = not detected. Each dot represents one measurement. The data are presented as the means ± SEMs. e Flow cytometric analysis of pSTAT3 levels in primary human stromal cells and human myeloid leukemic cell lines after huOSM treatment (2.5 h, 10 ng/mL). The mean fluorescence intensity (MFI) of the isotype control was subtracted from the pSTAT3 antibody MFI values. Each dot represents one measurement. The data are presented as the means ± SEMs. f , g OSM ( f ) and OSMR ( g ) expression across hematological and solid neoplasms utilizing Expression Public 24Q2 and lineage subtype grouping on the DepMap portal ( https://depmap.org )
Crl11346, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/32d/pmc05336186-210-8-7?v=ATCC
Average 93 stars, based on 1 article reviews
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plasma cleaned pdc-32d  (Harrick Plasma Inc)
90
Harrick Plasma Inc plasma cleaned pdc-32d
OSM is induced by STAT5-activating oncogenes in a kinase-dependent manner and acts on bone marrow stromal cells via STAT3 phosphorylation. a <t>32D</t> cells were transduced with various oncogenes and starved of cytokines and serum for twelve hours. Quantitative PCR revealed Osm upregulation in BCR::ABL1-, FLT3-ITD-, NPM1::ALK-, and JAK2 p.V617F-positive cells. Osm expression values were normalized to Gapdh and empty vector (MiG-empty) expression values. N ≥ 3 experiments; p values (compared with MiG-empty cells): *** p < 0.001; ** p < 0.01. The data are presented as the means ± SEMs. b 32D cells transduced with oncogenes were serum-starved plus inhibitor-treated as indicated for twelve hours. Osm expression values were normalized to Gapdh and MiG-empty vector expression values. P values (compared with MiG-empty cells): *** p < 0.001; ** p < 0.01. The data are presented as the means ± SEMs. c Flow cytometric analysis of OSMR surface levels in primary human stromal cells, primary AML blasts, and human myeloid leukemic cell lines. The mean fluorescence intensity (MFI) of the isotype control was subtracted from the OSMR MFI values. Each dot represents one measurement. The data are presented as the means ± SEMs. d Primary murine hematopoietic BM cells (HCs) did not express Osmr transcripts after 40 PCR cycles, whereas mesenchymal stromal cell (MSC) populations expressed Osmr . MSC = mesenchymal stromal cells, OB = osteoblasts, ND = not detected. Each dot represents one measurement. The data are presented as the means ± SEMs. e Flow cytometric analysis of pSTAT3 levels in primary human stromal cells and human myeloid leukemic cell lines after huOSM treatment (2.5 h, 10 ng/mL). The mean fluorescence intensity (MFI) of the isotype control was subtracted from the pSTAT3 antibody MFI values. Each dot represents one measurement. The data are presented as the means ± SEMs. f , g OSM ( f ) and OSMR ( g ) expression across hematological and solid neoplasms utilizing Expression Public 24Q2 and lineage subtype grouping on the DepMap portal ( https://depmap.org )
Plasma Cleaned Pdc 32d, supplied by Harrick Plasma Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/32d/pm37963943-199-7-8?v=Harrick+Plasma+Inc
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32d-gen cell lines  (Myelo Therapeutics GmbH)
90
Myelo Therapeutics GmbH 32d-gen cell lines
OSM is induced by STAT5-activating oncogenes in a kinase-dependent manner and acts on bone marrow stromal cells via STAT3 phosphorylation. a <t>32D</t> cells were transduced with various oncogenes and starved of cytokines and serum for twelve hours. Quantitative PCR revealed Osm upregulation in BCR::ABL1-, FLT3-ITD-, NPM1::ALK-, and JAK2 p.V617F-positive cells. Osm expression values were normalized to Gapdh and empty vector (MiG-empty) expression values. N ≥ 3 experiments; p values (compared with MiG-empty cells): *** p < 0.001; ** p < 0.01. The data are presented as the means ± SEMs. b 32D cells transduced with oncogenes were serum-starved plus inhibitor-treated as indicated for twelve hours. Osm expression values were normalized to Gapdh and MiG-empty vector expression values. P values (compared with MiG-empty cells): *** p < 0.001; ** p < 0.01. The data are presented as the means ± SEMs. c Flow cytometric analysis of OSMR surface levels in primary human stromal cells, primary AML blasts, and human myeloid leukemic cell lines. The mean fluorescence intensity (MFI) of the isotype control was subtracted from the OSMR MFI values. Each dot represents one measurement. The data are presented as the means ± SEMs. d Primary murine hematopoietic BM cells (HCs) did not express Osmr transcripts after 40 PCR cycles, whereas mesenchymal stromal cell (MSC) populations expressed Osmr . MSC = mesenchymal stromal cells, OB = osteoblasts, ND = not detected. Each dot represents one measurement. The data are presented as the means ± SEMs. e Flow cytometric analysis of pSTAT3 levels in primary human stromal cells and human myeloid leukemic cell lines after huOSM treatment (2.5 h, 10 ng/mL). The mean fluorescence intensity (MFI) of the isotype control was subtracted from the pSTAT3 antibody MFI values. Each dot represents one measurement. The data are presented as the means ± SEMs. f , g OSM ( f ) and OSMR ( g ) expression across hematological and solid neoplasms utilizing Expression Public 24Q2 and lineage subtype grouping on the DepMap portal ( https://depmap.org )
32d Gen Cell Lines, supplied by Myelo Therapeutics GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/32d/pm09519783-115-75-25?v=Myelo+Therapeutics+GmbH
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32d-gen cell lines - by Bioz Stars, 2026-07
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vit ek 2 e 32, d 16  (MBL Life science)
90
MBL Life science vit ek 2 e 32, d 16
OSM is induced by STAT5-activating oncogenes in a kinase-dependent manner and acts on bone marrow stromal cells via STAT3 phosphorylation. a <t>32D</t> cells were transduced with various oncogenes and starved of cytokines and serum for twelve hours. Quantitative PCR revealed Osm upregulation in BCR::ABL1-, FLT3-ITD-, NPM1::ALK-, and JAK2 p.V617F-positive cells. Osm expression values were normalized to Gapdh and empty vector (MiG-empty) expression values. N ≥ 3 experiments; p values (compared with MiG-empty cells): *** p < 0.001; ** p < 0.01. The data are presented as the means ± SEMs. b 32D cells transduced with oncogenes were serum-starved plus inhibitor-treated as indicated for twelve hours. Osm expression values were normalized to Gapdh and MiG-empty vector expression values. P values (compared with MiG-empty cells): *** p < 0.001; ** p < 0.01. The data are presented as the means ± SEMs. c Flow cytometric analysis of OSMR surface levels in primary human stromal cells, primary AML blasts, and human myeloid leukemic cell lines. The mean fluorescence intensity (MFI) of the isotype control was subtracted from the OSMR MFI values. Each dot represents one measurement. The data are presented as the means ± SEMs. d Primary murine hematopoietic BM cells (HCs) did not express Osmr transcripts after 40 PCR cycles, whereas mesenchymal stromal cell (MSC) populations expressed Osmr . MSC = mesenchymal stromal cells, OB = osteoblasts, ND = not detected. Each dot represents one measurement. The data are presented as the means ± SEMs. e Flow cytometric analysis of pSTAT3 levels in primary human stromal cells and human myeloid leukemic cell lines after huOSM treatment (2.5 h, 10 ng/mL). The mean fluorescence intensity (MFI) of the isotype control was subtracted from the pSTAT3 antibody MFI values. Each dot represents one measurement. The data are presented as the means ± SEMs. f , g OSM ( f ) and OSMR ( g ) expression across hematological and solid neoplasms utilizing Expression Public 24Q2 and lineage subtype grouping on the DepMap portal ( https://depmap.org )
Vit Ek 2 E 32, D 16, supplied by MBL Life science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/32d/pmc11001782__mmc3-42-36-19?v=MBL+Life+science
Average 90 stars, based on 1 article reviews
vit ek 2 e 32, d 16 - by Bioz Stars, 2026-07
90/100 stars
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nucleofector kits for 32d cells  (Lonza)
90
Lonza nucleofector kits for 32d cells
OSM is induced by STAT5-activating oncogenes in a kinase-dependent manner and acts on bone marrow stromal cells via STAT3 phosphorylation. a <t>32D</t> cells were transduced with various oncogenes and starved of cytokines and serum for twelve hours. Quantitative PCR revealed Osm upregulation in BCR::ABL1-, FLT3-ITD-, NPM1::ALK-, and JAK2 p.V617F-positive cells. Osm expression values were normalized to Gapdh and empty vector (MiG-empty) expression values. N ≥ 3 experiments; p values (compared with MiG-empty cells): *** p < 0.001; ** p < 0.01. The data are presented as the means ± SEMs. b 32D cells transduced with oncogenes were serum-starved plus inhibitor-treated as indicated for twelve hours. Osm expression values were normalized to Gapdh and MiG-empty vector expression values. P values (compared with MiG-empty cells): *** p < 0.001; ** p < 0.01. The data are presented as the means ± SEMs. c Flow cytometric analysis of OSMR surface levels in primary human stromal cells, primary AML blasts, and human myeloid leukemic cell lines. The mean fluorescence intensity (MFI) of the isotype control was subtracted from the OSMR MFI values. Each dot represents one measurement. The data are presented as the means ± SEMs. d Primary murine hematopoietic BM cells (HCs) did not express Osmr transcripts after 40 PCR cycles, whereas mesenchymal stromal cell (MSC) populations expressed Osmr . MSC = mesenchymal stromal cells, OB = osteoblasts, ND = not detected. Each dot represents one measurement. The data are presented as the means ± SEMs. e Flow cytometric analysis of pSTAT3 levels in primary human stromal cells and human myeloid leukemic cell lines after huOSM treatment (2.5 h, 10 ng/mL). The mean fluorescence intensity (MFI) of the isotype control was subtracted from the pSTAT3 antibody MFI values. Each dot represents one measurement. The data are presented as the means ± SEMs. f , g OSM ( f ) and OSMR ( g ) expression across hematological and solid neoplasms utilizing Expression Public 24Q2 and lineage subtype grouping on the DepMap portal ( https://depmap.org )
Nucleofector Kits For 32d Cells, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/32d/10__1128_slash_mcb__00758___14-48-8-15?v=Lonza
Average 90 stars, based on 1 article reviews
nucleofector kits for 32d cells - by Bioz Stars, 2026-07
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video microscopy chamber dutscher sa 32d ¥ 7h  (DUTSCHER DOMINIQUE)
90
DUTSCHER DOMINIQUE video microscopy chamber dutscher sa 32d ¥ 7h
OSM is induced by STAT5-activating oncogenes in a kinase-dependent manner and acts on bone marrow stromal cells via STAT3 phosphorylation. a <t>32D</t> cells were transduced with various oncogenes and starved of cytokines and serum for twelve hours. Quantitative PCR revealed Osm upregulation in BCR::ABL1-, FLT3-ITD-, NPM1::ALK-, and JAK2 p.V617F-positive cells. Osm expression values were normalized to Gapdh and empty vector (MiG-empty) expression values. N ≥ 3 experiments; p values (compared with MiG-empty cells): *** p < 0.001; ** p < 0.01. The data are presented as the means ± SEMs. b 32D cells transduced with oncogenes were serum-starved plus inhibitor-treated as indicated for twelve hours. Osm expression values were normalized to Gapdh and MiG-empty vector expression values. P values (compared with MiG-empty cells): *** p < 0.001; ** p < 0.01. The data are presented as the means ± SEMs. c Flow cytometric analysis of OSMR surface levels in primary human stromal cells, primary AML blasts, and human myeloid leukemic cell lines. The mean fluorescence intensity (MFI) of the isotype control was subtracted from the OSMR MFI values. Each dot represents one measurement. The data are presented as the means ± SEMs. d Primary murine hematopoietic BM cells (HCs) did not express Osmr transcripts after 40 PCR cycles, whereas mesenchymal stromal cell (MSC) populations expressed Osmr . MSC = mesenchymal stromal cells, OB = osteoblasts, ND = not detected. Each dot represents one measurement. The data are presented as the means ± SEMs. e Flow cytometric analysis of pSTAT3 levels in primary human stromal cells and human myeloid leukemic cell lines after huOSM treatment (2.5 h, 10 ng/mL). The mean fluorescence intensity (MFI) of the isotype control was subtracted from the pSTAT3 antibody MFI values. Each dot represents one measurement. The data are presented as the means ± SEMs. f , g OSM ( f ) and OSMR ( g ) expression across hematological and solid neoplasms utilizing Expression Public 24Q2 and lineage subtype grouping on the DepMap portal ( https://depmap.org )
Video Microscopy Chamber Dutscher Sa 32d ¥ 7h, supplied by DUTSCHER DOMINIQUE, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/32d/pm16008578-195-7-10?v=DUTSCHER+DOMINIQUE
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video microscopy chamber dutscher sa 32d ¥ 7h - by Bioz Stars, 2026-07
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cell line 32d-bcl-2  (Quelle GmbH)
90
Quelle GmbH cell line 32d-bcl-2
OSM is induced by STAT5-activating oncogenes in a kinase-dependent manner and acts on bone marrow stromal cells via STAT3 phosphorylation. a <t>32D</t> cells were transduced with various oncogenes and starved of cytokines and serum for twelve hours. Quantitative PCR revealed Osm upregulation in BCR::ABL1-, FLT3-ITD-, NPM1::ALK-, and JAK2 p.V617F-positive cells. Osm expression values were normalized to Gapdh and empty vector (MiG-empty) expression values. N ≥ 3 experiments; p values (compared with MiG-empty cells): *** p < 0.001; ** p < 0.01. The data are presented as the means ± SEMs. b 32D cells transduced with oncogenes were serum-starved plus inhibitor-treated as indicated for twelve hours. Osm expression values were normalized to Gapdh and MiG-empty vector expression values. P values (compared with MiG-empty cells): *** p < 0.001; ** p < 0.01. The data are presented as the means ± SEMs. c Flow cytometric analysis of OSMR surface levels in primary human stromal cells, primary AML blasts, and human myeloid leukemic cell lines. The mean fluorescence intensity (MFI) of the isotype control was subtracted from the OSMR MFI values. Each dot represents one measurement. The data are presented as the means ± SEMs. d Primary murine hematopoietic BM cells (HCs) did not express Osmr transcripts after 40 PCR cycles, whereas mesenchymal stromal cell (MSC) populations expressed Osmr . MSC = mesenchymal stromal cells, OB = osteoblasts, ND = not detected. Each dot represents one measurement. The data are presented as the means ± SEMs. e Flow cytometric analysis of pSTAT3 levels in primary human stromal cells and human myeloid leukemic cell lines after huOSM treatment (2.5 h, 10 ng/mL). The mean fluorescence intensity (MFI) of the isotype control was subtracted from the pSTAT3 antibody MFI values. Each dot represents one measurement. The data are presented as the means ± SEMs. f , g OSM ( f ) and OSMR ( g ) expression across hematological and solid neoplasms utilizing Expression Public 24Q2 and lineage subtype grouping on the DepMap portal ( https://depmap.org )
Cell Line 32d Bcl 2, supplied by Quelle GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/32d/pmc00316716-293-30-18?v=Quelle+GmbH
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cell line 32d-bcl-2 - by Bioz Stars, 2026-07
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32d-il-2/15rβ (32dβ) cells  (Altor BioScience)
90
Altor BioScience 32d-il-2/15rβ (32dβ) cells
OSM is induced by STAT5-activating oncogenes in a kinase-dependent manner and acts on bone marrow stromal cells via STAT3 phosphorylation. a <t>32D</t> cells were transduced with various oncogenes and starved of cytokines and serum for twelve hours. Quantitative PCR revealed Osm upregulation in BCR::ABL1-, FLT3-ITD-, NPM1::ALK-, and JAK2 p.V617F-positive cells. Osm expression values were normalized to Gapdh and empty vector (MiG-empty) expression values. N ≥ 3 experiments; p values (compared with MiG-empty cells): *** p < 0.001; ** p < 0.01. The data are presented as the means ± SEMs. b 32D cells transduced with oncogenes were serum-starved plus inhibitor-treated as indicated for twelve hours. Osm expression values were normalized to Gapdh and MiG-empty vector expression values. P values (compared with MiG-empty cells): *** p < 0.001; ** p < 0.01. The data are presented as the means ± SEMs. c Flow cytometric analysis of OSMR surface levels in primary human stromal cells, primary AML blasts, and human myeloid leukemic cell lines. The mean fluorescence intensity (MFI) of the isotype control was subtracted from the OSMR MFI values. Each dot represents one measurement. The data are presented as the means ± SEMs. d Primary murine hematopoietic BM cells (HCs) did not express Osmr transcripts after 40 PCR cycles, whereas mesenchymal stromal cell (MSC) populations expressed Osmr . MSC = mesenchymal stromal cells, OB = osteoblasts, ND = not detected. Each dot represents one measurement. The data are presented as the means ± SEMs. e Flow cytometric analysis of pSTAT3 levels in primary human stromal cells and human myeloid leukemic cell lines after huOSM treatment (2.5 h, 10 ng/mL). The mean fluorescence intensity (MFI) of the isotype control was subtracted from the pSTAT3 antibody MFI values. Each dot represents one measurement. The data are presented as the means ± SEMs. f , g OSM ( f ) and OSMR ( g ) expression across hematological and solid neoplasms utilizing Expression Public 24Q2 and lineage subtype grouping on the DepMap portal ( https://depmap.org )
32d Il 2/15rβ (32dβ) Cells, supplied by Altor BioScience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/32d/pmc08889352-169-1-7?v=Altor+BioScience
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32d-il-2/15rβ (32dβ) cells - by Bioz Stars, 2026-07
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32d cells  (Multiplexion GmbH)
90
Multiplexion GmbH 32d cells
OSM is induced by STAT5-activating oncogenes in a kinase-dependent manner and acts on bone marrow stromal cells via STAT3 phosphorylation. a <t>32D</t> cells were transduced with various oncogenes and starved of cytokines and serum for twelve hours. Quantitative PCR revealed Osm upregulation in BCR::ABL1-, FLT3-ITD-, NPM1::ALK-, and JAK2 p.V617F-positive cells. Osm expression values were normalized to Gapdh and empty vector (MiG-empty) expression values. N ≥ 3 experiments; p values (compared with MiG-empty cells): *** p < 0.001; ** p < 0.01. The data are presented as the means ± SEMs. b 32D cells transduced with oncogenes were serum-starved plus inhibitor-treated as indicated for twelve hours. Osm expression values were normalized to Gapdh and MiG-empty vector expression values. P values (compared with MiG-empty cells): *** p < 0.001; ** p < 0.01. The data are presented as the means ± SEMs. c Flow cytometric analysis of OSMR surface levels in primary human stromal cells, primary AML blasts, and human myeloid leukemic cell lines. The mean fluorescence intensity (MFI) of the isotype control was subtracted from the OSMR MFI values. Each dot represents one measurement. The data are presented as the means ± SEMs. d Primary murine hematopoietic BM cells (HCs) did not express Osmr transcripts after 40 PCR cycles, whereas mesenchymal stromal cell (MSC) populations expressed Osmr . MSC = mesenchymal stromal cells, OB = osteoblasts, ND = not detected. Each dot represents one measurement. The data are presented as the means ± SEMs. e Flow cytometric analysis of pSTAT3 levels in primary human stromal cells and human myeloid leukemic cell lines after huOSM treatment (2.5 h, 10 ng/mL). The mean fluorescence intensity (MFI) of the isotype control was subtracted from the pSTAT3 antibody MFI values. Each dot represents one measurement. The data are presented as the means ± SEMs. f , g OSM ( f ) and OSMR ( g ) expression across hematological and solid neoplasms utilizing Expression Public 24Q2 and lineage subtype grouping on the DepMap portal ( https://depmap.org )
32d Cells, supplied by Multiplexion GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


OSM is induced by STAT5-activating oncogenes in a kinase-dependent manner and acts on bone marrow stromal cells via STAT3 phosphorylation. a 32D cells were transduced with various oncogenes and starved of cytokines and serum for twelve hours. Quantitative PCR revealed Osm upregulation in BCR::ABL1-, FLT3-ITD-, NPM1::ALK-, and JAK2 p.V617F-positive cells. Osm expression values were normalized to Gapdh and empty vector (MiG-empty) expression values. N ≥ 3 experiments; p values (compared with MiG-empty cells): *** p < 0.001; ** p < 0.01. The data are presented as the means ± SEMs. b 32D cells transduced with oncogenes were serum-starved plus inhibitor-treated as indicated for twelve hours. Osm expression values were normalized to Gapdh and MiG-empty vector expression values. P values (compared with MiG-empty cells): *** p < 0.001; ** p < 0.01. The data are presented as the means ± SEMs. c Flow cytometric analysis of OSMR surface levels in primary human stromal cells, primary AML blasts, and human myeloid leukemic cell lines. The mean fluorescence intensity (MFI) of the isotype control was subtracted from the OSMR MFI values. Each dot represents one measurement. The data are presented as the means ± SEMs. d Primary murine hematopoietic BM cells (HCs) did not express Osmr transcripts after 40 PCR cycles, whereas mesenchymal stromal cell (MSC) populations expressed Osmr . MSC = mesenchymal stromal cells, OB = osteoblasts, ND = not detected. Each dot represents one measurement. The data are presented as the means ± SEMs. e Flow cytometric analysis of pSTAT3 levels in primary human stromal cells and human myeloid leukemic cell lines after huOSM treatment (2.5 h, 10 ng/mL). The mean fluorescence intensity (MFI) of the isotype control was subtracted from the pSTAT3 antibody MFI values. Each dot represents one measurement. The data are presented as the means ± SEMs. f , g OSM ( f ) and OSMR ( g ) expression across hematological and solid neoplasms utilizing Expression Public 24Q2 and lineage subtype grouping on the DepMap portal ( https://depmap.org )

Journal: Signal Transduction and Targeted Therapy

Article Title: Oncostatin M induced by STAT5-activating oncogenes promotes disease progression in hematologic malignancies

doi: 10.1038/s41392-025-02491-6

Figure Lengend Snippet: OSM is induced by STAT5-activating oncogenes in a kinase-dependent manner and acts on bone marrow stromal cells via STAT3 phosphorylation. a 32D cells were transduced with various oncogenes and starved of cytokines and serum for twelve hours. Quantitative PCR revealed Osm upregulation in BCR::ABL1-, FLT3-ITD-, NPM1::ALK-, and JAK2 p.V617F-positive cells. Osm expression values were normalized to Gapdh and empty vector (MiG-empty) expression values. N ≥ 3 experiments; p values (compared with MiG-empty cells): *** p < 0.001; ** p < 0.01. The data are presented as the means ± SEMs. b 32D cells transduced with oncogenes were serum-starved plus inhibitor-treated as indicated for twelve hours. Osm expression values were normalized to Gapdh and MiG-empty vector expression values. P values (compared with MiG-empty cells): *** p < 0.001; ** p < 0.01. The data are presented as the means ± SEMs. c Flow cytometric analysis of OSMR surface levels in primary human stromal cells, primary AML blasts, and human myeloid leukemic cell lines. The mean fluorescence intensity (MFI) of the isotype control was subtracted from the OSMR MFI values. Each dot represents one measurement. The data are presented as the means ± SEMs. d Primary murine hematopoietic BM cells (HCs) did not express Osmr transcripts after 40 PCR cycles, whereas mesenchymal stromal cell (MSC) populations expressed Osmr . MSC = mesenchymal stromal cells, OB = osteoblasts, ND = not detected. Each dot represents one measurement. The data are presented as the means ± SEMs. e Flow cytometric analysis of pSTAT3 levels in primary human stromal cells and human myeloid leukemic cell lines after huOSM treatment (2.5 h, 10 ng/mL). The mean fluorescence intensity (MFI) of the isotype control was subtracted from the pSTAT3 antibody MFI values. Each dot represents one measurement. The data are presented as the means ± SEMs. f , g OSM ( f ) and OSMR ( g ) expression across hematological and solid neoplasms utilizing Expression Public 24Q2 and lineage subtype grouping on the DepMap portal ( https://depmap.org )

Article Snippet: 32D, NIH/3T3 and OP9 cells were obtained from the German Resource Centre for Biological Material (DSMZ).

Techniques: Phospho-proteomics, Transduction, Real-time Polymerase Chain Reaction, Expressing, Plasmid Preparation, Fluorescence, Control