Journal: Mediators of Inflammation

Article Title: Antagonistic Peptides That Specifically Bind to the First and Second Extracellular Loops of CCR5 and Anti-IL-23p19 Antibody Reduce Airway Inflammation by Suppressing the IL-23/Th17 Signaling Pathway

doi: 10.1155/2020/1719467

Figure Lengend Snippet: Effects of peptides of CCR5 and anti-IL-23p19 mAb on the airway inflammation. (a)–(g) show photomicrographs of HE staining (original magnification: 400×) of lung sections in the control group, sensitization group, model group, anti-IL-23p19 group, GH group, HY group, and anti-IL-23p19 with the HY group, respectively. (h) Inflammation score of mouse lung tissue in each group. Values are the means ± SD ( n = 8). ∗ P < 0.05 compared with the model group.

Article Snippet: The IL-23p19, IL-17A, and CCR5 antibodies were purchased from Santa Cruz Biotechnology (Dallas, TA, USA).

Techniques: Staining, Control

Journal: Mediators of Inflammation

Article Title: Antagonistic Peptides That Specifically Bind to the First and Second Extracellular Loops of CCR5 and Anti-IL-23p19 Antibody Reduce Airway Inflammation by Suppressing the IL-23/Th17 Signaling Pathway

doi: 10.1155/2020/1719467

Figure Lengend Snippet: Effects of peptides of CCR5 and anti-IL-23p19 mAb on the airway mucus secretion. (a)–(g) show photomicrographs of PAS staining (original magnification: 400x) of lung sections in the control group, sensitization group, model group, anti-IL-23p19 group, GH group, HY group, and anti-IL-23p19 with the HY group, respectively. (h) Mucus hypersecretion score of mouse lung tissue in each group. Values are the means ± SD ( n = 8). ∗ P < 0.05 compared with the model group.

Article Snippet: The IL-23p19, IL-17A, and CCR5 antibodies were purchased from Santa Cruz Biotechnology (Dallas, TA, USA).

Techniques: Staining, Control

Journal: Mediators of Inflammation

Article Title: Antagonistic Peptides That Specifically Bind to the First and Second Extracellular Loops of CCR5 and Anti-IL-23p19 Antibody Reduce Airway Inflammation by Suppressing the IL-23/Th17 Signaling Pathway

doi: 10.1155/2020/1719467

Figure Lengend Snippet: Effects of peptides of CCR5 and anti-IL-23p19 mAb on the inflammatory cell counts and level of IL-4 in the BALF. (a) Flow cytometry gating strategy for inflammatory cell populations in the BALF. Absolute cell counts (b) and cellular composition (c) in the BALF. (d) Quantification of IL-4 in the BALF by means of ELISA. Values are the means ± SD ( n = 8). ∗ P < 0.05 compared with the model group.

Article Snippet: The IL-23p19, IL-17A, and CCR5 antibodies were purchased from Santa Cruz Biotechnology (Dallas, TA, USA).

Techniques: Flow Cytometry, Enzyme-linked Immunosorbent Assay

Journal: Mediators of Inflammation

Article Title: Antagonistic Peptides That Specifically Bind to the First and Second Extracellular Loops of CCR5 and Anti-IL-23p19 Antibody Reduce Airway Inflammation by Suppressing the IL-23/Th17 Signaling Pathway

doi: 10.1155/2020/1719467

Figure Lengend Snippet: Effects of peptides of CCR5 and anti-IL-23p19 mAb on the expression of IL-23p19, IL-23R, IL-17A, and LTF. (a) IL-23p19, IL-23R, IL-17A, and LTF mRNA levels compared with each other among the 7 groups. (b) Fold change of expression levels of the IL-23p19, IL-23R, IL-17A, and LTF proteins. C: control group; S: sensitization group; M: model group; 23: anti-IL-23p19 group; G: GH group; H: HY group; and 23+H: anti-IL-23p19 with the HY group. Values are the means ± SD ( n = 8). ∗ P < 0.05 compared with the model group. # P < 0.05 compared with the anti-IL-23p19+HY group. △ P < 0.05 compared with the HY group.

Article Snippet: The IL-23p19, IL-17A, and CCR5 antibodies were purchased from Santa Cruz Biotechnology (Dallas, TA, USA).

Techniques: Expressing, Control

Journal: Mediators of Inflammation

Article Title: Antagonistic Peptides That Specifically Bind to the First and Second Extracellular Loops of CCR5 and Anti-IL-23p19 Antibody Reduce Airway Inflammation by Suppressing the IL-23/Th17 Signaling Pathway

doi: 10.1155/2020/1719467

Figure Lengend Snippet: Effects of peptides of CCR5 and anti-IL-23p19 mAb on the number of Th17 cells positively produced. (a) Representative results of the percentage of the Th17 cells in the CD3 + CD4 + gate of the spleen compared the (i) control, (ii) sensitization, (iii) model, (iv) anti-IL-23p19, (v) GH, (vi) HY, and (vii) anti-IL-23p19 with HY groups, as analyzed by using flow cytometry. (vii) The FMO control of IL-17 staining. (b) Histogram showing the proportion of Th17 cells in the thymus, spleen, and peripheral blood. Values are the means ± SD ( n = 8). ∗ P < 0.05 compared with the model group. # P < 0.05 compared with the anti-IL-23p19+HY group.

Article Snippet: The IL-23p19, IL-17A, and CCR5 antibodies were purchased from Santa Cruz Biotechnology (Dallas, TA, USA).

Techniques: Produced, Control, Flow Cytometry, Staining

Journal: Mediators of Inflammation

Article Title: Antagonistic Peptides That Specifically Bind to the First and Second Extracellular Loops of CCR5 and Anti-IL-23p19 Antibody Reduce Airway Inflammation by Suppressing the IL-23/Th17 Signaling Pathway

doi: 10.1155/2020/1719467

Figure Lengend Snippet: Effects of peptides of CCR5 and anti-IL-23p19 mAb on the relationship between CCR5 and IL-23p19. CCR5 mRNA was analyzed by PCR (a), and CCR5 protein was analyzed by western blotting (b) after the administration of the peptides and/or anti-IL-23p19 to mice. C: control group; S: sensitization group; M: model group; 23: anti-IL-23p19 group; G: GH group; H: HY group; 23+H: anti-IL-23p19 with HY group. Values are the means ± SD ( n = 8). ∗ P < 0.05 compared with the model group.

Article Snippet: The IL-23p19, IL-17A, and CCR5 antibodies were purchased from Santa Cruz Biotechnology (Dallas, TA, USA).

Techniques: Western Blot, Control

Journal: Mediators of Inflammation

Article Title: Antagonistic Peptides That Specifically Bind to the First and Second Extracellular Loops of CCR5 and Anti-IL-23p19 Antibody Reduce Airway Inflammation by Suppressing the IL-23/Th17 Signaling Pathway

doi: 10.1155/2020/1719467

Figure Lengend Snippet: Correlation between the expression of the CCR5 and IL-23p19 proteins in the treatment groups ( n = 8).

Article Snippet: The IL-23p19, IL-17A, and CCR5 antibodies were purchased from Santa Cruz Biotechnology (Dallas, TA, USA).

Techniques: Expressing