32160 Search Results


c 4 2 kp 2210291 mcr 1  (ATCC)
90
ATCC c 4 2 kp 2210291 mcr 1
C 4 2 Kp 2210291 Mcr 1, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/c 4 2 kp 2210291 mcr 1/product/ATCC
Average 90 stars, based on 1 article reviews
c 4 2 kp 2210291 mcr 1 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
k63r  (Addgene inc)
86
Addgene inc k63r
a, Parkin WT and KO MEFs were treated with T/C/Z for 4 h. Ubiquitinated proteins were pull down under denaturing conditions by Ni-NTA agarose and analyzed by immunoblot. b , Cells were transfected with the indicated plasmids (Ubiquitin: WT, K6, K11, K27, K29, K33, K48, or K63 only) and then treated with T/C/Z for 4 h. Cell lysates were then subjected to IP and immunoblotted as indicated. SE, short exposure; LE, long exposure. c-d, Cells were transfected with the indicated plasmids (Ubiquitin: WT, K33R, K48R, <t>K63R,</t> or K33 only) and then treated with T/C/Z for 4 h. Cell lysates were then subjected to IP and immunoblotted as indicated. SE, short exposure; LE, long exposure. e, Cells were transfected with the indicated constructs (Parkin WT, R420H, or H461R) and then treated with T/C/Z. Ubiquitinated proteins were pulled down under denaturing conditions by Ni-NTA agarose and analyzed by immunoblot. f-g, U2OS cells were transfected with the indicated constructs and then treated with T/C/Z for 4 h. The distribution of RIPK3 (Red) was detected using immunofluorescence (f). Quantification of RIPK3 punctation (g). Scale bar, 20 μm (magnification, 200 μm). All experiments were repeated independently at least three times with similar results. Source data are provided in . Uncropped images of blots for (a,b,c,d,e) are shown in . Scale bars, 20 μm (magnification, 200 μm, f).
K63r, supplied by Addgene inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/k63r/product/Addgene inc
Average 86 stars, based on 1 article reviews
k63r - by Bioz Stars, 2026-03
86/100 stars
  Buy from Supplier

Image Search Results


a, Parkin WT and KO MEFs were treated with T/C/Z for 4 h. Ubiquitinated proteins were pull down under denaturing conditions by Ni-NTA agarose and analyzed by immunoblot. b , Cells were transfected with the indicated plasmids (Ubiquitin: WT, K6, K11, K27, K29, K33, K48, or K63 only) and then treated with T/C/Z for 4 h. Cell lysates were then subjected to IP and immunoblotted as indicated. SE, short exposure; LE, long exposure. c-d, Cells were transfected with the indicated plasmids (Ubiquitin: WT, K33R, K48R, K63R, or K33 only) and then treated with T/C/Z for 4 h. Cell lysates were then subjected to IP and immunoblotted as indicated. SE, short exposure; LE, long exposure. e, Cells were transfected with the indicated constructs (Parkin WT, R420H, or H461R) and then treated with T/C/Z. Ubiquitinated proteins were pulled down under denaturing conditions by Ni-NTA agarose and analyzed by immunoblot. f-g, U2OS cells were transfected with the indicated constructs and then treated with T/C/Z for 4 h. The distribution of RIPK3 (Red) was detected using immunofluorescence (f). Quantification of RIPK3 punctation (g). Scale bar, 20 μm (magnification, 200 μm). All experiments were repeated independently at least three times with similar results. Source data are provided in . Uncropped images of blots for (a,b,c,d,e) are shown in . Scale bars, 20 μm (magnification, 200 μm, f).

Journal: Nature cell biology

Article Title: The AMPK-Parkin axis negatively regulates necroptosis and tumorigenesis by inhibiting the necrosome

doi: 10.1038/s41556-019-0356-8

Figure Lengend Snippet: a, Parkin WT and KO MEFs were treated with T/C/Z for 4 h. Ubiquitinated proteins were pull down under denaturing conditions by Ni-NTA agarose and analyzed by immunoblot. b , Cells were transfected with the indicated plasmids (Ubiquitin: WT, K6, K11, K27, K29, K33, K48, or K63 only) and then treated with T/C/Z for 4 h. Cell lysates were then subjected to IP and immunoblotted as indicated. SE, short exposure; LE, long exposure. c-d, Cells were transfected with the indicated plasmids (Ubiquitin: WT, K33R, K48R, K63R, or K33 only) and then treated with T/C/Z for 4 h. Cell lysates were then subjected to IP and immunoblotted as indicated. SE, short exposure; LE, long exposure. e, Cells were transfected with the indicated constructs (Parkin WT, R420H, or H461R) and then treated with T/C/Z. Ubiquitinated proteins were pulled down under denaturing conditions by Ni-NTA agarose and analyzed by immunoblot. f-g, U2OS cells were transfected with the indicated constructs and then treated with T/C/Z for 4 h. The distribution of RIPK3 (Red) was detected using immunofluorescence (f). Quantification of RIPK3 punctation (g). Scale bar, 20 μm (magnification, 200 μm). All experiments were repeated independently at least three times with similar results. Source data are provided in . Uncropped images of blots for (a,b,c,d,e) are shown in . Scale bars, 20 μm (magnification, 200 μm, f).

Article Snippet: HA-tagged ubiquitin and ubiquitin lysine mutants, such as K-6 only, K-11 only, K-27 only, K-29 only, K-33 only, K-48 only, K-63 only, K33R, K48R, and K63R were obtained from Addgene.

Techniques: Western Blot, Transfection, Ubiquitin Proteomics, Construct, Immunofluorescence