31333 Search Results


npra  (Novus Biologicals)
92
Novus Biologicals npra
Npra, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/npra/product/Novus Biologicals
Average 92 stars, based on 1 article reviews
npra - by Bioz Stars, 2026-02
92/100 stars
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psmd11  (Addgene inc)
88
Addgene inc psmd11
UBE3AT485A depletes multiple endogenous proteasome subunits. A, protein lysates from HEK293T cells expressing UBE3AT485A or UBE3AT485E mutants were analyzed by Western blotting for proteasome subunit protein levels. Analysis was performed on cells grown in L-cell CM or Wnt3a CM. B and C, quantification of Western blots for cells grown in L-cell CM (B) or Wnt3a CM (C). Mean percent abundance values in UBE3AT485A samples relative to UBE3AT485E samples are shown (n = 6). Error bars indicate S.D. Statistical analysis was performed using a one-sample t test (two-tailed). *, p < 0.05; **, p < 0.005. <t>PSMD11</t> was not analyzed due to its low abundance in HEK293T cells. D and E, representative Western blot (D) and quantification (E) of proteasome subunits in immortalized lymphocyte cell lines from the father, mother, and autism proband (Simon's Simplex Collection; Family ID 13873). Mean percent values are shown relative to the father's level (n = 3). Error bars indicate S.D. *, p < 0.05, one-sample t test.
Psmd11, supplied by Addgene inc, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/psmd11/product/Addgene inc
Average 88 stars, based on 1 article reviews
psmd11 - by Bioz Stars, 2026-02
88/100 stars
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31333-45  (Nacalai)
90
Nacalai 31333-45
UBE3AT485A depletes multiple endogenous proteasome subunits. A, protein lysates from HEK293T cells expressing UBE3AT485A or UBE3AT485E mutants were analyzed by Western blotting for proteasome subunit protein levels. Analysis was performed on cells grown in L-cell CM or Wnt3a CM. B and C, quantification of Western blots for cells grown in L-cell CM (B) or Wnt3a CM (C). Mean percent abundance values in UBE3AT485A samples relative to UBE3AT485E samples are shown (n = 6). Error bars indicate S.D. Statistical analysis was performed using a one-sample t test (two-tailed). *, p < 0.05; **, p < 0.005. <t>PSMD11</t> was not analyzed due to its low abundance in HEK293T cells. D and E, representative Western blot (D) and quantification (E) of proteasome subunits in immortalized lymphocyte cell lines from the father, mother, and autism proband (Simon's Simplex Collection; Family ID 13873). Mean percent values are shown relative to the father's level (n = 3). Error bars indicate S.D. *, p < 0.05, one-sample t test.
31333 45, supplied by Nacalai, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/31333-45/product/Nacalai
Average 90 stars, based on 1 article reviews
31333-45 - by Bioz Stars, 2026-02
90/100 stars
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Image Search Results


UBE3AT485A depletes multiple endogenous proteasome subunits. A, protein lysates from HEK293T cells expressing UBE3AT485A or UBE3AT485E mutants were analyzed by Western blotting for proteasome subunit protein levels. Analysis was performed on cells grown in L-cell CM or Wnt3a CM. B and C, quantification of Western blots for cells grown in L-cell CM (B) or Wnt3a CM (C). Mean percent abundance values in UBE3AT485A samples relative to UBE3AT485E samples are shown (n = 6). Error bars indicate S.D. Statistical analysis was performed using a one-sample t test (two-tailed). *, p < 0.05; **, p < 0.005. PSMD11 was not analyzed due to its low abundance in HEK293T cells. D and E, representative Western blot (D) and quantification (E) of proteasome subunits in immortalized lymphocyte cell lines from the father, mother, and autism proband (Simon's Simplex Collection; Family ID 13873). Mean percent values are shown relative to the father's level (n = 3). Error bars indicate S.D. *, p < 0.05, one-sample t test.

Journal: The Journal of Biological Chemistry

Article Title: The autism-linked UBE3A T485A mutant E3 ubiquitin ligase activates the Wnt/β-catenin pathway by inhibiting the proteasome

doi: 10.1074/jbc.M117.788448

Figure Lengend Snippet: UBE3AT485A depletes multiple endogenous proteasome subunits. A, protein lysates from HEK293T cells expressing UBE3AT485A or UBE3AT485E mutants were analyzed by Western blotting for proteasome subunit protein levels. Analysis was performed on cells grown in L-cell CM or Wnt3a CM. B and C, quantification of Western blots for cells grown in L-cell CM (B) or Wnt3a CM (C). Mean percent abundance values in UBE3AT485A samples relative to UBE3AT485E samples are shown (n = 6). Error bars indicate S.D. Statistical analysis was performed using a one-sample t test (two-tailed). *, p < 0.05; **, p < 0.005. PSMD11 was not analyzed due to its low abundance in HEK293T cells. D and E, representative Western blot (D) and quantification (E) of proteasome subunits in immortalized lymphocyte cell lines from the father, mother, and autism proband (Simon's Simplex Collection; Family ID 13873). Mean percent values are shown relative to the father's level (n = 3). Error bars indicate S.D. *, p < 0.05, one-sample t test.

Article Snippet: Ub G76V -GFP was obtained from Addgene (23969). pCMV6 Myc-DDK-tagged constructs for PSMB1 (RC201798), PSMC2 (RC200945), PSMC5 (RC201251), PSMC6 (RC202809), PSMD1 (RC210486), PSMD2 (RC203204), PSMD3 (RC202307), PSMD6 (RC202292), PSMD7 (RC203133), PSMD11 (RC201201), and untagged PSMD4 (SC111678) were all purchased from Origene.

Techniques: Expressing, Western Blot, Two Tailed Test