3090 Search Results


mrsa ccarm 3090  (ATCC)
95
ATCC mrsa ccarm 3090
Mrsa Ccarm 3090, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gallein  (Tocris)
95
Tocris gallein
Gallein, supplied by Tocris, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tyrosine hydroxylase  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology tyrosine hydroxylase
Down regulation of the dopamine biosynthesis pathway in the PC12 model of Huntington's disease . A : qRT-PCR was performed on samples from Q 23 and Q74 cells for 0, 1 and 5 days. All values are expressed as relative mRNA levels compared to expression levels of uninduced cells. SPR: Sepiapterin reductase, GCH1: GTP cyclohydroxylase 1, TH: Tyrosine <t>hydroxylase,</t> DDC: Dopa decarboxylase, DBH: Dopamine β-hydroxylase. * Significant differences between the day 1 and 5 days; * P < 0.05, ** P < 0.005, *** P < 0.0005 student's t-test. Significant differences between Q23 on day 1 and Q74 on day 1; + P < 0.05, ++ P < 0.005 student's t-test. B : Confocal microscopic images of Q23 and Q74 PC12 cells after 5 days of induction. Green represents huntingtin-GFP and red represents TH immunohistochemistry. In Q23 cells, both huntingtin and TH show a diffuse staining, while in Q74 cells, large aggregates are visible and a clear reduction of TH fluorescence. Scale bar = 25 μm. C : Western blot analysis on whole cell lysates of Q23 and Q74 cells with TH and β-actin as loading control. A clear reduction in TH level can be seen in the 5 day Q74 samples. D : HPLC measurements in whole cell lysates of Q23 and Q74 cells after 0, 1 and 5 days of huntingtin expression show a significant reduction in dopamine levels in the 5 day Q74 samples. (* P < 0.05, student's t-test).
Tyrosine Hydroxylase, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse anti rat kappa hrp  (SouthernBiotech)
93
SouthernBiotech mouse anti rat kappa hrp
Down regulation of the dopamine biosynthesis pathway in the PC12 model of Huntington's disease . A : qRT-PCR was performed on samples from Q 23 and Q74 cells for 0, 1 and 5 days. All values are expressed as relative mRNA levels compared to expression levels of uninduced cells. SPR: Sepiapterin reductase, GCH1: GTP cyclohydroxylase 1, TH: Tyrosine <t>hydroxylase,</t> DDC: Dopa decarboxylase, DBH: Dopamine β-hydroxylase. * Significant differences between the day 1 and 5 days; * P < 0.05, ** P < 0.005, *** P < 0.0005 student's t-test. Significant differences between Q23 on day 1 and Q74 on day 1; + P < 0.05, ++ P < 0.005 student's t-test. B : Confocal microscopic images of Q23 and Q74 PC12 cells after 5 days of induction. Green represents huntingtin-GFP and red represents TH immunohistochemistry. In Q23 cells, both huntingtin and TH show a diffuse staining, while in Q74 cells, large aggregates are visible and a clear reduction of TH fluorescence. Scale bar = 25 μm. C : Western blot analysis on whole cell lysates of Q23 and Q74 cells with TH and β-actin as loading control. A clear reduction in TH level can be seen in the 5 day Q74 samples. D : HPLC measurements in whole cell lysates of Q23 and Q74 cells after 0, 1 and 5 days of huntingtin expression show a significant reduction in dopamine levels in the 5 day Q74 samples. (* P < 0.05, student's t-test).
Mouse Anti Rat Kappa Hrp, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti pyk2  (Cell Signaling Technology Inc)
91
Cell Signaling Technology Inc anti pyk2
Down regulation of the dopamine biosynthesis pathway in the PC12 model of Huntington's disease . A : qRT-PCR was performed on samples from Q 23 and Q74 cells for 0, 1 and 5 days. All values are expressed as relative mRNA levels compared to expression levels of uninduced cells. SPR: Sepiapterin reductase, GCH1: GTP cyclohydroxylase 1, TH: Tyrosine <t>hydroxylase,</t> DDC: Dopa decarboxylase, DBH: Dopamine β-hydroxylase. * Significant differences between the day 1 and 5 days; * P < 0.05, ** P < 0.005, *** P < 0.0005 student's t-test. Significant differences between Q23 on day 1 and Q74 on day 1; + P < 0.05, ++ P < 0.005 student's t-test. B : Confocal microscopic images of Q23 and Q74 PC12 cells after 5 days of induction. Green represents huntingtin-GFP and red represents TH immunohistochemistry. In Q23 cells, both huntingtin and TH show a diffuse staining, while in Q74 cells, large aggregates are visible and a clear reduction of TH fluorescence. Scale bar = 25 μm. C : Western blot analysis on whole cell lysates of Q23 and Q74 cells with TH and β-actin as loading control. A clear reduction in TH level can be seen in the 5 day Q74 samples. D : HPLC measurements in whole cell lysates of Q23 and Q74 cells after 0, 1 and 5 days of huntingtin expression show a significant reduction in dopamine levels in the 5 day Q74 samples. (* P < 0.05, student's t-test).
Anti Pyk2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pcy3090 02  (Addgene inc)
93
Addgene inc pcy3090 02
Down regulation of the dopamine biosynthesis pathway in the PC12 model of Huntington's disease . A : qRT-PCR was performed on samples from Q 23 and Q74 cells for 0, 1 and 5 days. All values are expressed as relative mRNA levels compared to expression levels of uninduced cells. SPR: Sepiapterin reductase, GCH1: GTP cyclohydroxylase 1, TH: Tyrosine <t>hydroxylase,</t> DDC: Dopa decarboxylase, DBH: Dopamine β-hydroxylase. * Significant differences between the day 1 and 5 days; * P < 0.05, ** P < 0.005, *** P < 0.0005 student's t-test. Significant differences between Q23 on day 1 and Q74 on day 1; + P < 0.05, ++ P < 0.005 student's t-test. B : Confocal microscopic images of Q23 and Q74 PC12 cells after 5 days of induction. Green represents huntingtin-GFP and red represents TH immunohistochemistry. In Q23 cells, both huntingtin and TH show a diffuse staining, while in Q74 cells, large aggregates are visible and a clear reduction of TH fluorescence. Scale bar = 25 μm. C : Western blot analysis on whole cell lysates of Q23 and Q74 cells with TH and β-actin as loading control. A clear reduction in TH level can be seen in the 5 day Q74 samples. D : HPLC measurements in whole cell lysates of Q23 and Q74 cells after 0, 1 and 5 days of huntingtin expression show a significant reduction in dopamine levels in the 5 day Q74 samples. (* P < 0.05, student's t-test).
Pcy3090 02, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse kappa ab  (SouthernBiotech)
93
SouthernBiotech mouse kappa ab
Down regulation of the dopamine biosynthesis pathway in the PC12 model of Huntington's disease . A : qRT-PCR was performed on samples from Q 23 and Q74 cells for 0, 1 and 5 days. All values are expressed as relative mRNA levels compared to expression levels of uninduced cells. SPR: Sepiapterin reductase, GCH1: GTP cyclohydroxylase 1, TH: Tyrosine <t>hydroxylase,</t> DDC: Dopa decarboxylase, DBH: Dopamine β-hydroxylase. * Significant differences between the day 1 and 5 days; * P < 0.05, ** P < 0.005, *** P < 0.0005 student's t-test. Significant differences between Q23 on day 1 and Q74 on day 1; + P < 0.05, ++ P < 0.005 student's t-test. B : Confocal microscopic images of Q23 and Q74 PC12 cells after 5 days of induction. Green represents huntingtin-GFP and red represents TH immunohistochemistry. In Q23 cells, both huntingtin and TH show a diffuse staining, while in Q74 cells, large aggregates are visible and a clear reduction of TH fluorescence. Scale bar = 25 μm. C : Western blot analysis on whole cell lysates of Q23 and Q74 cells with TH and β-actin as loading control. A clear reduction in TH level can be seen in the 5 day Q74 samples. D : HPLC measurements in whole cell lysates of Q23 and Q74 cells after 0, 1 and 5 days of huntingtin expression show a significant reduction in dopamine levels in the 5 day Q74 samples. (* P < 0.05, student's t-test).
Mouse Kappa Ab, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti kappa pe  (SouthernBiotech)
85
SouthernBiotech anti kappa pe
Down regulation of the dopamine biosynthesis pathway in the PC12 model of Huntington's disease . A : qRT-PCR was performed on samples from Q 23 and Q74 cells for 0, 1 and 5 days. All values are expressed as relative mRNA levels compared to expression levels of uninduced cells. SPR: Sepiapterin reductase, GCH1: GTP cyclohydroxylase 1, TH: Tyrosine <t>hydroxylase,</t> DDC: Dopa decarboxylase, DBH: Dopamine β-hydroxylase. * Significant differences between the day 1 and 5 days; * P < 0.05, ** P < 0.005, *** P < 0.0005 student's t-test. Significant differences between Q23 on day 1 and Q74 on day 1; + P < 0.05, ++ P < 0.005 student's t-test. B : Confocal microscopic images of Q23 and Q74 PC12 cells after 5 days of induction. Green represents huntingtin-GFP and red represents TH immunohistochemistry. In Q23 cells, both huntingtin and TH show a diffuse staining, while in Q74 cells, large aggregates are visible and a clear reduction of TH fluorescence. Scale bar = 25 μm. C : Western blot analysis on whole cell lysates of Q23 and Q74 cells with TH and β-actin as loading control. A clear reduction in TH level can be seen in the 5 day Q74 samples. D : HPLC measurements in whole cell lysates of Q23 and Q74 cells after 0, 1 and 5 days of huntingtin expression show a significant reduction in dopamine levels in the 5 day Q74 samples. (* P < 0.05, student's t-test).
Anti Kappa Pe, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plasmid pcy3090 07  (Addgene inc)
91
Addgene inc plasmid pcy3090 07
Down regulation of the dopamine biosynthesis pathway in the PC12 model of Huntington's disease . A : qRT-PCR was performed on samples from Q 23 and Q74 cells for 0, 1 and 5 days. All values are expressed as relative mRNA levels compared to expression levels of uninduced cells. SPR: Sepiapterin reductase, GCH1: GTP cyclohydroxylase 1, TH: Tyrosine <t>hydroxylase,</t> DDC: Dopa decarboxylase, DBH: Dopamine β-hydroxylase. * Significant differences between the day 1 and 5 days; * P < 0.05, ** P < 0.005, *** P < 0.0005 student's t-test. Significant differences between Q23 on day 1 and Q74 on day 1; + P < 0.05, ++ P < 0.005 student's t-test. B : Confocal microscopic images of Q23 and Q74 PC12 cells after 5 days of induction. Green represents huntingtin-GFP and red represents TH immunohistochemistry. In Q23 cells, both huntingtin and TH show a diffuse staining, while in Q74 cells, large aggregates are visible and a clear reduction of TH fluorescence. Scale bar = 25 μm. C : Western blot analysis on whole cell lysates of Q23 and Q74 cells with TH and β-actin as loading control. A clear reduction in TH level can be seen in the 5 day Q74 samples. D : HPLC measurements in whole cell lysates of Q23 and Q74 cells after 0, 1 and 5 days of huntingtin expression show a significant reduction in dopamine levels in the 5 day Q74 samples. (* P < 0.05, student's t-test).
Plasmid Pcy3090 07, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fitc rat anti mouse kappa  (SouthernBiotech)
92
SouthernBiotech fitc rat anti mouse kappa
Down regulation of the dopamine biosynthesis pathway in the PC12 model of Huntington's disease . A : qRT-PCR was performed on samples from Q 23 and Q74 cells for 0, 1 and 5 days. All values are expressed as relative mRNA levels compared to expression levels of uninduced cells. SPR: Sepiapterin reductase, GCH1: GTP cyclohydroxylase 1, TH: Tyrosine <t>hydroxylase,</t> DDC: Dopa decarboxylase, DBH: Dopamine β-hydroxylase. * Significant differences between the day 1 and 5 days; * P < 0.05, ** P < 0.005, *** P < 0.0005 student's t-test. Significant differences between Q23 on day 1 and Q74 on day 1; + P < 0.05, ++ P < 0.005 student's t-test. B : Confocal microscopic images of Q23 and Q74 PC12 cells after 5 days of induction. Green represents huntingtin-GFP and red represents TH immunohistochemistry. In Q23 cells, both huntingtin and TH show a diffuse staining, while in Q74 cells, large aggregates are visible and a clear reduction of TH fluorescence. Scale bar = 25 μm. C : Western blot analysis on whole cell lysates of Q23 and Q74 cells with TH and β-actin as loading control. A clear reduction in TH level can be seen in the 5 day Q74 samples. D : HPLC measurements in whole cell lysates of Q23 and Q74 cells after 0, 1 and 5 days of huntingtin expression show a significant reduction in dopamine levels in the 5 day Q74 samples. (* P < 0.05, student's t-test).
Fitc Rat Anti Mouse Kappa, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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aspeuc1 309019 a tubingensis  (ATCC)
91
ATCC aspeuc1 309019 a tubingensis
Down regulation of the dopamine biosynthesis pathway in the PC12 model of Huntington's disease . A : qRT-PCR was performed on samples from Q 23 and Q74 cells for 0, 1 and 5 days. All values are expressed as relative mRNA levels compared to expression levels of uninduced cells. SPR: Sepiapterin reductase, GCH1: GTP cyclohydroxylase 1, TH: Tyrosine <t>hydroxylase,</t> DDC: Dopa decarboxylase, DBH: Dopamine β-hydroxylase. * Significant differences between the day 1 and 5 days; * P < 0.05, ** P < 0.005, *** P < 0.0005 student's t-test. Significant differences between Q23 on day 1 and Q74 on day 1; + P < 0.05, ++ P < 0.005 student's t-test. B : Confocal microscopic images of Q23 and Q74 PC12 cells after 5 days of induction. Green represents huntingtin-GFP and red represents TH immunohistochemistry. In Q23 cells, both huntingtin and TH show a diffuse staining, while in Q74 cells, large aggregates are visible and a clear reduction of TH fluorescence. Scale bar = 25 μm. C : Western blot analysis on whole cell lysates of Q23 and Q74 cells with TH and β-actin as loading control. A clear reduction in TH level can be seen in the 5 day Q74 samples. D : HPLC measurements in whole cell lysates of Q23 and Q74 cells after 0, 1 and 5 days of huntingtin expression show a significant reduction in dopamine levels in the 5 day Q74 samples. (* P < 0.05, student's t-test).
Aspeuc1 309019 A Tubingensis, supplied by ATCC, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Down regulation of the dopamine biosynthesis pathway in the PC12 model of Huntington's disease . A : qRT-PCR was performed on samples from Q 23 and Q74 cells for 0, 1 and 5 days. All values are expressed as relative mRNA levels compared to expression levels of uninduced cells. SPR: Sepiapterin reductase, GCH1: GTP cyclohydroxylase 1, TH: Tyrosine hydroxylase, DDC: Dopa decarboxylase, DBH: Dopamine β-hydroxylase. * Significant differences between the day 1 and 5 days; * P < 0.05, ** P < 0.005, *** P < 0.0005 student's t-test. Significant differences between Q23 on day 1 and Q74 on day 1; + P < 0.05, ++ P < 0.005 student's t-test. B : Confocal microscopic images of Q23 and Q74 PC12 cells after 5 days of induction. Green represents huntingtin-GFP and red represents TH immunohistochemistry. In Q23 cells, both huntingtin and TH show a diffuse staining, while in Q74 cells, large aggregates are visible and a clear reduction of TH fluorescence. Scale bar = 25 μm. C : Western blot analysis on whole cell lysates of Q23 and Q74 cells with TH and β-actin as loading control. A clear reduction in TH level can be seen in the 5 day Q74 samples. D : HPLC measurements in whole cell lysates of Q23 and Q74 cells after 0, 1 and 5 days of huntingtin expression show a significant reduction in dopamine levels in the 5 day Q74 samples. (* P < 0.05, student's t-test).

Journal: BMC Molecular Biology

Article Title: Mutant huntingtin activates Nrf2-responsive genes and impairs dopamine synthesis in a PC12 model of Huntington's disease

doi: 10.1186/1471-2199-9-84

Figure Lengend Snippet: Down regulation of the dopamine biosynthesis pathway in the PC12 model of Huntington's disease . A : qRT-PCR was performed on samples from Q 23 and Q74 cells for 0, 1 and 5 days. All values are expressed as relative mRNA levels compared to expression levels of uninduced cells. SPR: Sepiapterin reductase, GCH1: GTP cyclohydroxylase 1, TH: Tyrosine hydroxylase, DDC: Dopa decarboxylase, DBH: Dopamine β-hydroxylase. * Significant differences between the day 1 and 5 days; * P < 0.05, ** P < 0.005, *** P < 0.0005 student's t-test. Significant differences between Q23 on day 1 and Q74 on day 1; + P < 0.05, ++ P < 0.005 student's t-test. B : Confocal microscopic images of Q23 and Q74 PC12 cells after 5 days of induction. Green represents huntingtin-GFP and red represents TH immunohistochemistry. In Q23 cells, both huntingtin and TH show a diffuse staining, while in Q74 cells, large aggregates are visible and a clear reduction of TH fluorescence. Scale bar = 25 μm. C : Western blot analysis on whole cell lysates of Q23 and Q74 cells with TH and β-actin as loading control. A clear reduction in TH level can be seen in the 5 day Q74 samples. D : HPLC measurements in whole cell lysates of Q23 and Q74 cells after 0, 1 and 5 days of huntingtin expression show a significant reduction in dopamine levels in the 5 day Q74 samples. (* P < 0.05, student's t-test).

Article Snippet: Cells were fixed in 4% paraformaldehyde for 15 min and permeabilized in 50% methanol and 1% H 2 O 2 for 20 min. After three washes in PBS containing 0.2% Triton X-100 (PBS-Triton), cells were incubated overnight at 4°C with rabbit anti tyrosine hydroxylase (1:2000, Santa Cruz Biotechnology Inc., USA) in PBS-Triton with 1% normal goat serum and 0.4% Thimerosal (Sigma, St. Louis, USA).

Techniques: Quantitative RT-PCR, Expressing, Immunohistochemistry, Staining, Fluorescence, Western Blot, Control