Journal: International Journal of Microbiology

Article Title: Antifungal Activity of Selected Indigenous Pseudomonas and Bacillus from the Soybean Rhizosphere

doi: 10.1155/2009/572049

Figure Lengend Snippet: Bacterial species source and origin.

Article Snippet: B. amyloliquefaciens DSM1060 , DSMZ †.

Techniques:

Journal: bioRxiv

Article Title: pH-dependent spontaneous hydrolysis rather than gut bacterial metabolism reduces levels of the ADHD treatment, Methylphenidate

doi: 10.1101/2020.07.06.189191

Figure Lengend Snippet: (A) MPH hydrolysis in growth medium prepared at different pH values. (B, C) pH effect on MPH hydrolysis in E. faecium W54 (B) , and E. coli BW25113 (C) (black bars; right y-axis) shown as the ratio of [RA] 24h /[MPH] 0h quantified in ng/μL and normalized to d10-RA and pH measurements after 24 h of incubation with 50 μM MPH (grey bars; right y axis).

Article Snippet: L. salivarius W1, L. plantarum W24 and E. faecium W54 strains were obtained from Winclove Probiotic B.V. C. ammoniagenes DSM20306, E. coli DSM1058 and E. coli DSM12250 were obtained from the German Collection of Microorganisms and Cell Cultures (DSMZ).

Techniques: Incubation

Journal: bioRxiv

Article Title: pH-dependent spontaneous hydrolysis rather than gut bacterial metabolism reduces levels of the ADHD treatment, Methylphenidate

doi: 10.1101/2020.07.06.189191

Figure Lengend Snippet: Optical density measured as the absorbance at 600 nm is plotted over time in aerobic cultures of (A) E. coli strains (BW25113, DSM11250 and DSM1058) grown at 37 °C, 220 rpm; (B) Enterococcus strains ( E. faecalis V583 and E. faecium W54) strains grown at 37 °C without agitation (C) Lactobacillus strains ( L. plantarum W1 and L. salivarius W24) grown at 37 °C, 220 rpm Enterococcus strains ( E. faecalis V583 and E. faecium W54) strains grown at 37 °C without agitation; (D) C. ammoniagenes DSM20306 grown at 37 °C, 220 rpm and (E) P. fluorescens MFY63 grown at 37 °C, 220 rpm. Arrows indicate the late exponential phase when MPH was added.

Article Snippet: L. salivarius W1, L. plantarum W24 and E. faecium W54 strains were obtained from Winclove Probiotic B.V. C. ammoniagenes DSM20306, E. coli DSM1058 and E. coli DSM12250 were obtained from the German Collection of Microorganisms and Cell Cultures (DSMZ).

Techniques:

Journal: bioRxiv

Article Title: pH-dependent spontaneous hydrolysis rather than gut bacterial metabolism reduces levels of the ADHD treatment, Methylphenidate

doi: 10.1101/2020.07.06.189191

Figure Lengend Snippet: (A, B) Calibration curves obtained in the two different biological matrices used in this study: (A) pure bacterial cultures of E. coli BW25113 and (B) pool of small intestinal content of 5 WTG rats. Peak areas of methylphenidate (MPH) and ritalinic acid (RA) are normalized to the peak area of the internal standard d10-Ritalinic acid (d10-RA). (C) Linearity of the calibration curves fitted with a linear regression model. Data represents 3 biological replicates and error bars represent standard deviation.

Article Snippet: L. salivarius W1, L. plantarum W24 and E. faecium W54 strains were obtained from Winclove Probiotic B.V. C. ammoniagenes DSM20306, E. coli DSM1058 and E. coli DSM12250 were obtained from the German Collection of Microorganisms and Cell Cultures (DSMZ).

Techniques: Standard Deviation

Journal: bioRxiv

Article Title: pH-dependent spontaneous hydrolysis rather than gut bacterial metabolism reduces levels of the ADHD treatment, Methylphenidate

doi: 10.1101/2020.07.06.189191

Figure Lengend Snippet: (A) Phylogenetic trees created using iTOL online tool showing gut bacterial strains harboring homologue enzymes of human CES1, E. coli yjfP and B. subtilis pnbA respectively. (B) Screening of gut bacterial pure cultures for the metabolism of MPH. Metabolic activity is shown as the ratio of [RA] 24h /[MPH] 0h quantified in ng/μL and normalized to d10-RA (black bars; left y-axis) together with pH measurements in the cultures after 24 h of incubation (grey bars; right y-axis) with 50 μM MPH. Error bars represent standard deviation (n = 3).

Article Snippet: L. salivarius W1, L. plantarum W24 and E. faecium W54 strains were obtained from Winclove Probiotic B.V. C. ammoniagenes DSM20306, E. coli DSM1058 and E. coli DSM12250 were obtained from the German Collection of Microorganisms and Cell Cultures (DSMZ).

Techniques: Activity Assay, Incubation, Standard Deviation

Journal: bioRxiv

Article Title: pH-dependent spontaneous hydrolysis rather than gut bacterial metabolism reduces levels of the ADHD treatment, Methylphenidate

doi: 10.1101/2020.07.06.189191

Figure Lengend Snippet: MPH hydrolysis in supernatants of (A) E. faecium W54 and (B) E. coli BW25113 after adjusting the pH to different values. MPH hydrolysis in bacterial supernatants at different pH values, shown as the ratio of [RA] 24h /[MPH] 0h quantified in ng/μL and normalized to d10-RA after 24 h of incubation with 50 μM MPH.

Article Snippet: L. salivarius W1, L. plantarum W24 and E. faecium W54 strains were obtained from Winclove Probiotic B.V. C. ammoniagenes DSM20306, E. coli DSM1058 and E. coli DSM12250 were obtained from the German Collection of Microorganisms and Cell Cultures (DSMZ).

Techniques: Incubation