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staphylococcus use  (ATCC)
90
ATCC staphylococcus use
Staphylococcus Use, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/staphylococcus use/product/ATCC
Average 90 stars, based on 1 article reviews
staphylococcus use - by Bioz Stars, 2026-02
90/100 stars
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anti stat5b antibody  (R&D Systems)
86
R&D Systems anti stat5b antibody
Fig. 7. STAT5 and Runx induce transcription of TCRc genes in a DP cell line. (A) DPK cells were transduced with indicated retrovirus, cultured with or without IL-7 for 72 h and analyzed for surface expression of CD4 and CD8 (left panels) and IL-7Ra (right histograms). Histograms show IL- 7Ra expression gated on CD4+CD8+ (DP) cells (left) and CD4CD8+ (8SP) cells (right), respectively. In histograms, thin and bold lines indicate cells with or without IL-7 stimulation, respectively. Shaded areas indicate isotype control. (B and C) Total RNA from indicated DPK cells cultured with or without IL-7 for 72 h was analyzed by PCR for the presence of germline (B) and rearranged Vc-Jc (C) transcripts. Genomic DNA from DPK cells or intraepithelial lymphocytes (IEL) served as controls. The data represent one of four independent experiments with similar results. (D–F) DPK cells were transduced with mock, WT- or <t>CA-STAT5a</t> retrovirus. Cells were cultured and analyzed as described in A–C.
Anti Stat5b Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti stat5b antibody/product/R&D Systems
Average 86 stars, based on 1 article reviews
anti stat5b antibody - by Bioz Stars, 2026-02
86/100 stars
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pdsred2 c1 vector  (Addgene inc)
86
Addgene inc pdsred2 c1 vector
Fig. 7. STAT5 and Runx induce transcription of TCRc genes in a DP cell line. (A) DPK cells were transduced with indicated retrovirus, cultured with or without IL-7 for 72 h and analyzed for surface expression of CD4 and CD8 (left panels) and IL-7Ra (right histograms). Histograms show IL- 7Ra expression gated on CD4+CD8+ (DP) cells (left) and CD4CD8+ (8SP) cells (right), respectively. In histograms, thin and bold lines indicate cells with or without IL-7 stimulation, respectively. Shaded areas indicate isotype control. (B and C) Total RNA from indicated DPK cells cultured with or without IL-7 for 72 h was analyzed by PCR for the presence of germline (B) and rearranged Vc-Jc (C) transcripts. Genomic DNA from DPK cells or intraepithelial lymphocytes (IEL) served as controls. The data represent one of four independent experiments with similar results. (D–F) DPK cells were transduced with mock, WT- or <t>CA-STAT5a</t> retrovirus. Cells were cultured and analyzed as described in A–C.
Pdsred2 C1 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pdsred2 c1 vector/product/Addgene inc
Average 86 stars, based on 1 article reviews
pdsred2 c1 vector - by Bioz Stars, 2026-02
86/100 stars
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Image Search Results


Fig. 7. STAT5 and Runx induce transcription of TCRc genes in a DP cell line. (A) DPK cells were transduced with indicated retrovirus, cultured with or without IL-7 for 72 h and analyzed for surface expression of CD4 and CD8 (left panels) and IL-7Ra (right histograms). Histograms show IL- 7Ra expression gated on CD4+CD8+ (DP) cells (left) and CD4CD8+ (8SP) cells (right), respectively. In histograms, thin and bold lines indicate cells with or without IL-7 stimulation, respectively. Shaded areas indicate isotype control. (B and C) Total RNA from indicated DPK cells cultured with or without IL-7 for 72 h was analyzed by PCR for the presence of germline (B) and rearranged Vc-Jc (C) transcripts. Genomic DNA from DPK cells or intraepithelial lymphocytes (IEL) served as controls. The data represent one of four independent experiments with similar results. (D–F) DPK cells were transduced with mock, WT- or CA-STAT5a retrovirus. Cells were cultured and analyzed as described in A–C.

Journal: International immunology

Article Title: The pre-TCR signal induces transcriptional silencing of the TCRγ locus by reducing the recruitment of STAT5 and Runx to transcriptional enhancers.

doi: 10.1093/intimm/dxr055

Figure Lengend Snippet: Fig. 7. STAT5 and Runx induce transcription of TCRc genes in a DP cell line. (A) DPK cells were transduced with indicated retrovirus, cultured with or without IL-7 for 72 h and analyzed for surface expression of CD4 and CD8 (left panels) and IL-7Ra (right histograms). Histograms show IL- 7Ra expression gated on CD4+CD8+ (DP) cells (left) and CD4CD8+ (8SP) cells (right), respectively. In histograms, thin and bold lines indicate cells with or without IL-7 stimulation, respectively. Shaded areas indicate isotype control. (B and C) Total RNA from indicated DPK cells cultured with or without IL-7 for 72 h was analyzed by PCR for the presence of germline (B) and rearranged Vc-Jc (C) transcripts. Genomic DNA from DPK cells or intraepithelial lymphocytes (IEL) served as controls. The data represent one of four independent experiments with similar results. (D–F) DPK cells were transduced with mock, WT- or CA-STAT5a retrovirus. Cells were cultured and analyzed as described in A–C.

Article Snippet: Briefly, soluble chromatin consisting 200- to 1000-bp fragments was immunoprecipitated with antiacetylated histone H3 antibody (Upstate Biotechnology), rabbit anti-pan-Runx antibody (30), mixture of anti-STAT5A antibody and anti-STAT5B antibody (R&D Systems, Minneapolis, MN, USA) or an equal amount of normal rabbit IgG (Upstate Biotechnology) overnight at 4 C. Purified ChIP and input DNAs were measured by PCR and real-time quantitative PCR.

Techniques: Transduction, Cell Culture, Expressing, Control