Journal: Journal of Biological Chemistry

Article Title: Atypical Protein Kinase Cι Plays a Critical Role in Human Lung Cancer Cell Growth and Tumorigenicity

doi: 10.1074/jbc.m505402200

Figure Lengend Snippet: FIG. 2. PKC does not regulate NFB signaling in A549 cells. A, immunoblot analysis of A549 cell transfectants for cIAP2, Bcl-XL, and actin. B, analysis of A549 cell transfectants for PARP and cleaved PARP. HeLa cells treated with taxol for either 24 or 48 h served as a positive control for cleaved PARP. C, A549/pBabe and A549/kdPKC cells were treated with 50 g/ml TNF for the indicated times and cell lysates subjected to immunoblot analysis for detection of IB, phospho-(Ser32)-IB, and actin. D, A549/pBabe and A549/kdPKC cells were treated with 50 g/ml TNF for the indicated times and cell lysates subjected to immunoblot analysis for detection of NFB p65, phospho-(Ser536)-NFB, p65, and actin. E, transcriptional activity of an NFB-luciferase reporter in A549/pBabe and A549/kdPKC cells in the presence and absence of TNF. Data represent the mean S.E. of three independent determinations.

Article Snippet: This paper is available on line at http://www.jbc.org 31109 at K U N G L IG A T E K N ISK A H O G SK O L A N on M arch 8, 2015 http://w w w .jbc.org/ D ow nloaded from (Sigma, catalog number A8592); Rac1, 1:3000 (BD Transduction Laboratories, catalog number 610651); cIAP2, 1:500 (Santa Cruz Biotechnology, catalog number sc-7944); Bcl-XL, 1:1,000 (Cell Signaling Technologies, catalog number 2762); PARP/cleaved PARP, 1:1,000 (Cell Signaling Technologies, catalog number 9542); I B , 1:1,000 (Cell Signaling Technologies, catalog number 9242); phospho-(Ser32)-I B , 1:1,000 (Cell Signaling Technologies, catalog number 9241); NF B p65, 1:1,000 (Cell Signaling Technologies, catalog number 3034); phospho(Ser536)-NF B p65, 1:1,000 (Cell Signaling Technologies, catalog number 3031); Mek, phospho-(Ser217/221)-Mek, and phospho-(Ser298)Mek, each at 1:1,000 (Cell Signaling Technologies, catalog numbers 9122, 9121, and 9128, respectively), Erk and phospho-(Thr202/Tyr204)Erk, each at 1:1,000 (Cell Signaling Technologies, catalog numbers 9102 and 9101, respectively); CD31, 1:1,000 (or Pecam-1; Santa Cruz Biotechnology, catalog number sc-1506); BrdUrd, 1:100 (DAKO, catalog number M0744).

Techniques: Western Blot, Positive Control, Activity Assay, Luciferase

Journal: Antioxidants

Article Title: Regioselective and Stereoselective Epoxidation of n-3 and n-6 Fatty Acids by Fungal Peroxygenases

doi: 10.3390/antiox10121888

Figure Lengend Snippet: Formulae of the n-3 ( 1 – 8 ) and n-6 ( 9 – 11 ) fatty acids used in enzymatic epoxidation reactions: ( 1 ) Hexadeca-7,10,13-trienoic acid (HTA); ( 2 ) Octadeca-6,9,12,15-tetraenoic acid (SDA); ( 3 ) Eicosa-11,14,17-trienoic acid (ETE); ( 4 ) Eicosa-5,8,11,14,17-pentaenoic acid (EPA); ( 5 ) Heneicosa-6,9,12,15,18-pentaenoic acid (HPA); ( 6 ) Docosa-7,10,13,16,19-pentaenoic acid (DPA); ( 7 ) Docosa-4,7,10,13,16,19-hexaenoic acid (DHA); ( 8 ) Tetracosa-6,9,12,15,18,21-hexaenoic acid (nisinic acid); ( 9 ) Eicosa-8,11,14-trienoic acid (DGLA); ( 10 ) Eicosa-5,8,11,14-tetraenoic acid (AA); and ( 11 ) Docosa-7,10,13,16-tetraenoic acid (AdA).

Article Snippet: These compounds −namely hexadeca-7,10,13-trienoic acid (HTA; 1 ), octadeca-6,9,12,15-tetraenoic acid (stearidonic acid, SDA; 2 ), eicosa-11,14,17-trienoic acid (ETE; 3 ), eicosa-5,8,11,14,17-pentaenoic acid (EPA; 4 ), heneicosa-6,9,12,15,18-pentaenoic acid (HPA; 5 ), docosa-7,10,13,16,19-pentaenoic acid (DPA; 6 ), docosa-4,7,10,13,16,19-hexaenoic acid (DHA; 7 ), tetracosa-6,9,12,15,18,21-hexaenoic acid (nisinic acid; 8 ), eicosa-8,11,14-trienoic acid (dihomo-γ-linoleic acid, DGLA; 9 ), eicosa-5,8,11,14-tetraenoic acid (AA; 10 ) and docosa-7,10,13,16-tetraenoic acid (adrenic acid, AdA; 11 )− were supplied by Larodan ( cis isomers in all cases).

Techniques:

Journal: bioRxiv

Article Title: Arsenite methyltransferase 3 regulates hepatic energy metabolism which dictates the hepatic response to arsenic exposure

doi: 10.1101/2023.04.05.535637

Figure Lengend Snippet: A . Representative images of WT and as3mt -/- larvae and adults. B-C . Standard length of larvae (μM; B) and adults (cm; C). D . Average IC-ICP-MS counts of AsB, AsIII, DMA, and MMA from WT (grey) or as3mt -/- (pink) zebrafish extracts after 0 (dotted lines) or 1 mM iAs (6-120 hpf) challenge (solid lines) (n = 40, 2 clutches). E . Percent survival of WT (grey) and as3mt -/- (pink) across 0, 0.5, 1.0, 1.5 and 2.0 mM iAs exposure from 6-120 hpf. Intersect with green line denotes the LC 50 . n = ∼60, 3 clutches. F . Representative images of WT and as3mt -/- larvae during 0 mM, 1 mM acute (96-120 hpf), or 1 mM chronic (6-120 hpf) exposure to iAs. G . Images of 120 hpf zebrafish pigmentation at 0 mM or 1 mM chronic (6-120 hpf) iAs challenge (purple). Orange arrows mark pigmentation clusters. H . Quantification of pigmentation per larvae (n = 5-10, 1 clutch). I . Percent phenotype (normal (white), abnormal (grey) or dead (black) after 0-2 mM iAs exposure from 6-120 hpf (n = ∼60, 3 clutches).

Article Snippet: IC-ICP-MS standards were: As(III) (Arsenic (III) Standard, Fisher Scientific; 1327-53-3), As(V) (Arsenic V Speciation Standard, Fisher Scientfic; 7732-18-5, AsB (Arsenobetaine Standard Solution, LGC Standards; NIST-3033), DMA (Dimethylarsinic Acid Standard Solution, LGC Standards; NIST-3031) and MMA (Monomethylarsonic Acid Standard Solution, LGC Standards; NIST-3030).

Techniques: