2a 1 Search Results


mouse anti csp  (Developmental Studies Hybridoma Bank)
94
Developmental Studies Hybridoma Bank mouse anti csp
Mouse Anti Csp, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vinyl boronic ester  (Chem Impex International)
94
Chem Impex International vinyl boronic ester
Vinyl Boronic Ester, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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2a1 ci  (Developmental Studies Hybridoma Bank)
95
Developmental Studies Hybridoma Bank 2a1 ci
2a1 Ci, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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inhoffenstrasse 7 b  (DSMZ)
86
DSMZ inhoffenstrasse 7 b
Inhoffenstrasse 7 B, supplied by DSMZ, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti ralbp1  (Novus Biologicals)
91
Novus Biologicals anti ralbp1
Anti Ralbp1, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hy p3278a  (MedChemExpress)
93
MedChemExpress hy p3278a
Hy P3278a, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti gad  (Developmental Studies Hybridoma Bank)
94
Developmental Studies Hybridoma Bank anti gad
Anti Gad, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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2a1  (Hycult Biotech)
92
Hycult Biotech 2a1
2a1, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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p akt  (Bioss)
92
Bioss p akt
P Akt, supplied by Bioss, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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btn2a1 fc fusion protein r d systems  (R&D Systems)
92
R&D Systems btn2a1 fc fusion protein r d systems
Btn2a1 Fc Fusion Protein R D Systems, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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monoclonal anti bdnf  (Bioss)
94
Bioss monoclonal anti bdnf
Monoclonal Anti Bdnf, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti tfr1  (Hycult Biotech)
90
Hycult Biotech anti tfr1
HEK293 cells transiently transfected with a vector encoding either HA-SphK1 or HA-SphK2 together with control, SphK1-siRNA or SphK2-siRNA were cultured for 72 hr. After cell lysis, the lysates were subjected to SDS-PAGE followed by immunoblot analysis using anti-HA and anti-β-tubulin antibodies (A). K562 cells transfected with control, SphK1-siRNA or SphK2-siRNA were cultured for 48 hr. Cell medium was then changed to exosome-depleted one and cultured for 12 hr. Exosomes were purified from the media and analyzed for cargo contents by immunoblot analysis <t>using</t> <t>anti-TfR1,</t> anti-HSP70, and anti-flotillin 2 antibodies. Cell lysates were also subjected to immunoblot analysis with anti-flotillin 2 antibody (B).
Anti Tfr1, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


HEK293 cells transiently transfected with a vector encoding either HA-SphK1 or HA-SphK2 together with control, SphK1-siRNA or SphK2-siRNA were cultured for 72 hr. After cell lysis, the lysates were subjected to SDS-PAGE followed by immunoblot analysis using anti-HA and anti-β-tubulin antibodies (A). K562 cells transfected with control, SphK1-siRNA or SphK2-siRNA were cultured for 48 hr. Cell medium was then changed to exosome-depleted one and cultured for 12 hr. Exosomes were purified from the media and analyzed for cargo contents by immunoblot analysis using anti-TfR1, anti-HSP70, and anti-flotillin 2 antibodies. Cell lysates were also subjected to immunoblot analysis with anti-flotillin 2 antibody (B).

Journal: Kobe Journal of Medical Sciences

Article Title: Essential Role of Sphingosine Kinase 2 in the Regulation of Cargo Contents in the Exosomes from K562 Cells

doi:

Figure Lengend Snippet: HEK293 cells transiently transfected with a vector encoding either HA-SphK1 or HA-SphK2 together with control, SphK1-siRNA or SphK2-siRNA were cultured for 72 hr. After cell lysis, the lysates were subjected to SDS-PAGE followed by immunoblot analysis using anti-HA and anti-β-tubulin antibodies (A). K562 cells transfected with control, SphK1-siRNA or SphK2-siRNA were cultured for 48 hr. Cell medium was then changed to exosome-depleted one and cultured for 12 hr. Exosomes were purified from the media and analyzed for cargo contents by immunoblot analysis using anti-TfR1, anti-HSP70, and anti-flotillin 2 antibodies. Cell lysates were also subjected to immunoblot analysis with anti-flotillin 2 antibody (B).

Article Snippet: Anti-EEA1 antibody (catalog number 610456) was purchased from BD Biosciences; anti-TfR1 (catalog number HM2134) from Hycult Biotech; anti-HSP70 (catalog number SPA-815) from Stressgen Biotechnologies; anti-flotillin 2 (catalog number sc-48398) from Santa Cruz Biotechnology.

Techniques: Transfection, Plasmid Preparation, Cell Culture, Lysis, SDS Page, Western Blot, Purification

K562 cells transiently transfected with a vector encoding either siRNA-resistant HA-SphK2 or HA-SphK2(G248D) together with control or SphK2-siRNA were cultured for 48 hr. Exosomes were prepared as in Fig. 2B and analyzed for cargo contents by immunoblot analysis using anti-flotillin 2 antibody (A). K562 cells were cultured in exosome-depleted medium for 12 hr in the absence or presence of either 10 μM DMS or 50 μM HACPT. Exosomes were purified from the media and analyzed for cargo contents by immunoblot analysis using anti-TfR1, anti-HSP70, and anti-flotillin 2 antibodies (B).

Journal: Kobe Journal of Medical Sciences

Article Title: Essential Role of Sphingosine Kinase 2 in the Regulation of Cargo Contents in the Exosomes from K562 Cells

doi:

Figure Lengend Snippet: K562 cells transiently transfected with a vector encoding either siRNA-resistant HA-SphK2 or HA-SphK2(G248D) together with control or SphK2-siRNA were cultured for 48 hr. Exosomes were prepared as in Fig. 2B and analyzed for cargo contents by immunoblot analysis using anti-flotillin 2 antibody (A). K562 cells were cultured in exosome-depleted medium for 12 hr in the absence or presence of either 10 μM DMS or 50 μM HACPT. Exosomes were purified from the media and analyzed for cargo contents by immunoblot analysis using anti-TfR1, anti-HSP70, and anti-flotillin 2 antibodies (B).

Article Snippet: Anti-EEA1 antibody (catalog number 610456) was purchased from BD Biosciences; anti-TfR1 (catalog number HM2134) from Hycult Biotech; anti-HSP70 (catalog number SPA-815) from Stressgen Biotechnologies; anti-flotillin 2 (catalog number sc-48398) from Santa Cruz Biotechnology.

Techniques: Transfection, Plasmid Preparation, Cell Culture, Western Blot, Purification