29916 Search Results


atcc 29916  (ATCC)
92
ATCC atcc 29916
Atcc 29916, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/atcc 29916/product/ATCC
Average 92 stars, based on 1 article reviews
atcc 29916 - by Bioz Stars, 2026-02
92/100 stars
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ckiiβ  (Santa Cruz Biotechnology)
90
Santa Cruz Biotechnology ckiiβ
Silencing <t>the</t> <t>NMT1</t> or CKIIα genes blocked Tat.AG but not Tat.B-induced HIV-1 transactivation in U38 ( A – C ) and TZM-bl ( D – F ) cells. ( A , D ) RT-PCR confirmation of NMT1, NMT2, CKIIα and <t>CKIIβ</t> genes silencing. ( B , E ) Western blot confirmation of NMT1, NMT2, CKIIα and CKIIβ genes silencing. Control: non-transduced cells. S: cells transduced with scrambled shRNA. ( C , F ) Silencing the NMT1 or CKIIα genes blocked Tat.AG-induced increase in CAT enzyme levels ( C ) and luciferase activity ( F ), but had no effect on Tat.B-induced viral transactivation. Silencing the NMT2 or CKIIβ genes had no effect on Tat.AG or Tat.B-induced viral transactivation. *P < 0.05, **P < 0.01, # P < 0.0001, compared to cells treated with similar concentrations of scrambled (S) shRNA and Tat proteins. Errors bars represent SD.
Ckiiβ, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ckiiβ/product/Santa Cruz Biotechnology
Average 90 stars, based on 1 article reviews
ckiiβ - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
ck2β  (Santa Cruz Biotechnology)
90
Santa Cruz Biotechnology ck2β
Silencing <t>the</t> <t>NMT1</t> or CKIIα genes blocked Tat.AG but not Tat.B-induced HIV-1 transactivation in U38 ( A – C ) and TZM-bl ( D – F ) cells. ( A , D ) RT-PCR confirmation of NMT1, NMT2, CKIIα and <t>CKIIβ</t> genes silencing. ( B , E ) Western blot confirmation of NMT1, NMT2, CKIIα and CKIIβ genes silencing. Control: non-transduced cells. S: cells transduced with scrambled shRNA. ( C , F ) Silencing the NMT1 or CKIIα genes blocked Tat.AG-induced increase in CAT enzyme levels ( C ) and luciferase activity ( F ), but had no effect on Tat.B-induced viral transactivation. Silencing the NMT2 or CKIIβ genes had no effect on Tat.AG or Tat.B-induced viral transactivation. *P < 0.05, **P < 0.01, # P < 0.0001, compared to cells treated with similar concentrations of scrambled (S) shRNA and Tat proteins. Errors bars represent SD.
Ck2β, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ck2β/product/Santa Cruz Biotechnology
Average 90 stars, based on 1 article reviews
ck2β - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier

Image Search Results


Silencing the NMT1 or CKIIα genes blocked Tat.AG but not Tat.B-induced HIV-1 transactivation in U38 ( A – C ) and TZM-bl ( D – F ) cells. ( A , D ) RT-PCR confirmation of NMT1, NMT2, CKIIα and CKIIβ genes silencing. ( B , E ) Western blot confirmation of NMT1, NMT2, CKIIα and CKIIβ genes silencing. Control: non-transduced cells. S: cells transduced with scrambled shRNA. ( C , F ) Silencing the NMT1 or CKIIα genes blocked Tat.AG-induced increase in CAT enzyme levels ( C ) and luciferase activity ( F ), but had no effect on Tat.B-induced viral transactivation. Silencing the NMT2 or CKIIβ genes had no effect on Tat.AG or Tat.B-induced viral transactivation. *P < 0.05, **P < 0.01, # P < 0.0001, compared to cells treated with similar concentrations of scrambled (S) shRNA and Tat proteins. Errors bars represent SD.

Journal: Scientific Reports

Article Title: Epigenetics, N-myrystoyltransferase-1 and casein kinase-2-alpha modulates the increased replication of HIV-1 CRF02_AG, compared to subtype-B viruses

doi: 10.1038/s41598-019-47069-9

Figure Lengend Snippet: Silencing the NMT1 or CKIIα genes blocked Tat.AG but not Tat.B-induced HIV-1 transactivation in U38 ( A – C ) and TZM-bl ( D – F ) cells. ( A , D ) RT-PCR confirmation of NMT1, NMT2, CKIIα and CKIIβ genes silencing. ( B , E ) Western blot confirmation of NMT1, NMT2, CKIIα and CKIIβ genes silencing. Control: non-transduced cells. S: cells transduced with scrambled shRNA. ( C , F ) Silencing the NMT1 or CKIIα genes blocked Tat.AG-induced increase in CAT enzyme levels ( C ) and luciferase activity ( F ), but had no effect on Tat.B-induced viral transactivation. Silencing the NMT2 or CKIIβ genes had no effect on Tat.AG or Tat.B-induced viral transactivation. *P < 0.05, **P < 0.01, # P < 0.0001, compared to cells treated with similar concentrations of scrambled (S) shRNA and Tat proteins. Errors bars represent SD.

Article Snippet: Knockdown of NMT1, NMT2, CKIIα and CKIIβ genes was performed using transduction ready shRNA containing 3 target-specific constructs that encoded 19–25 nucleotide (plus hairpin) shRNA designed to knockdown gene expression: NMT1 (sc-61132-V), NMT2 (sc-61134-V), CKIIα (sc-29918-V), and CKIIβ (sc-29916-V) (Santa Cruz Biotechnology, Dallas, TX).

Techniques: Reverse Transcription Polymerase Chain Reaction, Western Blot, Control, Transduction, shRNA, Luciferase, Activity Assay

Silencing the NMT1 gene blocked the infection of human MDM by HIV-1 CRF02_AG isolates, but had no effect on clade-B HIV-1 infection. Silencing of NMT1, NMT2, CKIIα and CKIIβ genes in primary human MDM was confirmed by RT-PCR ( A ) and Western blot analyses ( B ). For panels A and B , controls are non-transduced MDM. “S” are MDM transduced with scrambled shRNA. Following NMT1 and NMT2 gene silencing, MDM were infected with CRF02_AG or clade-B HIV-1 isolates (3 different isolates for each subtype, each isolate tested in triplicate) and RT activity measured from day-5 to day-21 p.i. ( C , D ) RT activity in MDM with NMT1 gene silenced. ( E , F ) RT activity in MDM with NMT2 gene silenced. For panels C – F , controls are non-HIV infected (mock-infected) MDM, “S” are MDM transduced with scrambled shRNA; “sh” are MDM transduced with NMT1 or NMT2 shRNA. **P < 0.01; # P < 0.0001, compared to infected MDM treated with similar concentrations of scrambled shRNA (S).

Journal: Scientific Reports

Article Title: Epigenetics, N-myrystoyltransferase-1 and casein kinase-2-alpha modulates the increased replication of HIV-1 CRF02_AG, compared to subtype-B viruses

doi: 10.1038/s41598-019-47069-9

Figure Lengend Snippet: Silencing the NMT1 gene blocked the infection of human MDM by HIV-1 CRF02_AG isolates, but had no effect on clade-B HIV-1 infection. Silencing of NMT1, NMT2, CKIIα and CKIIβ genes in primary human MDM was confirmed by RT-PCR ( A ) and Western blot analyses ( B ). For panels A and B , controls are non-transduced MDM. “S” are MDM transduced with scrambled shRNA. Following NMT1 and NMT2 gene silencing, MDM were infected with CRF02_AG or clade-B HIV-1 isolates (3 different isolates for each subtype, each isolate tested in triplicate) and RT activity measured from day-5 to day-21 p.i. ( C , D ) RT activity in MDM with NMT1 gene silenced. ( E , F ) RT activity in MDM with NMT2 gene silenced. For panels C – F , controls are non-HIV infected (mock-infected) MDM, “S” are MDM transduced with scrambled shRNA; “sh” are MDM transduced with NMT1 or NMT2 shRNA. **P < 0.01; # P < 0.0001, compared to infected MDM treated with similar concentrations of scrambled shRNA (S).

Article Snippet: Knockdown of NMT1, NMT2, CKIIα and CKIIβ genes was performed using transduction ready shRNA containing 3 target-specific constructs that encoded 19–25 nucleotide (plus hairpin) shRNA designed to knockdown gene expression: NMT1 (sc-61132-V), NMT2 (sc-61134-V), CKIIα (sc-29918-V), and CKIIβ (sc-29916-V) (Santa Cruz Biotechnology, Dallas, TX).

Techniques: Infection, Reverse Transcription Polymerase Chain Reaction, Western Blot, Transduction, shRNA, Activity Assay

Silencing the CKIIα gene blocked the infection of human MDM by HIV-1 CRF02_AG isolates, but had no effect on clade-B HIV-1 infection. ( A – D ) Following silencing of CKIIα and CKIIβ genes, MDM were infected with CRF02_AG or clade-B HIV-1 isolates (3 different isolates for each subtype, each isolate tested in triplicate) and RT activity measured from day-5 to day-21 p.i. ( A , B ) RT activity in MDM with CKIIα gene silenced. ( C , D ) RT activity in MDM with CKIIβ gene silenced. Controls are non-HIV infected (mock-infected) MDM, “S” are MDM transduced with scrambled shRNA; “sh” are MDM transduced with CKIIα or CKIIβ shRNA. *P < 0.05, # P < 0.0001, compared to infected MDM transduced with similar concentrations of scrambled shRNA (S).

Journal: Scientific Reports

Article Title: Epigenetics, N-myrystoyltransferase-1 and casein kinase-2-alpha modulates the increased replication of HIV-1 CRF02_AG, compared to subtype-B viruses

doi: 10.1038/s41598-019-47069-9

Figure Lengend Snippet: Silencing the CKIIα gene blocked the infection of human MDM by HIV-1 CRF02_AG isolates, but had no effect on clade-B HIV-1 infection. ( A – D ) Following silencing of CKIIα and CKIIβ genes, MDM were infected with CRF02_AG or clade-B HIV-1 isolates (3 different isolates for each subtype, each isolate tested in triplicate) and RT activity measured from day-5 to day-21 p.i. ( A , B ) RT activity in MDM with CKIIα gene silenced. ( C , D ) RT activity in MDM with CKIIβ gene silenced. Controls are non-HIV infected (mock-infected) MDM, “S” are MDM transduced with scrambled shRNA; “sh” are MDM transduced with CKIIα or CKIIβ shRNA. *P < 0.05, # P < 0.0001, compared to infected MDM transduced with similar concentrations of scrambled shRNA (S).

Article Snippet: Knockdown of NMT1, NMT2, CKIIα and CKIIβ genes was performed using transduction ready shRNA containing 3 target-specific constructs that encoded 19–25 nucleotide (plus hairpin) shRNA designed to knockdown gene expression: NMT1 (sc-61132-V), NMT2 (sc-61134-V), CKIIα (sc-29918-V), and CKIIβ (sc-29916-V) (Santa Cruz Biotechnology, Dallas, TX).

Techniques: Infection, Activity Assay, Transduction, shRNA