Journal: Scientific reports

Article Title: Regulation of MYB mediated cisplatin resistance of ovarian cancer cells involves miR-21-wnt signaling axis.

doi: 10.1038/s41598-020-63396-8

Figure Lengend Snippet: Figure 4. miR-21 reverses effect of c-MYB silencing on wnt signaling. (A) Levels of miR-21 were assessed, by qRT-PCR, in two ovarian cancer cell lines (ES2 and OVCAR3). Expression of miR-21 in control cells was set as 1 (black bars) and the altered expression of miR-21 in c-MYB silenced cells is shown as gray bars. Wnt signaling was assessed through phosphorylation of β-catenin, as detected by ELISA, and endpoint measurement of absorbance at 450 nm. ES2 (B) and OVCAR3 (C) were transfected either with siRNA against c-MYB alone or with pre-miR-21, and then β-catenin was detected by ELISA. *p < 0.05 and #p < 0.01, compared to control.

Article Snippet: Cell lines were cultured in 5% CO2 humidified incubator with the temperature set to 37 °C. c-MYB and miR-21 transfections. c-myb cloned in pCMV6-XL5 was purchased from Origene and transfected using TurboFectin transfection reagent while siRNA against c-myb was purchased from SCBT (China).

Techniques: Quantitative RT-PCR, Expressing, Control, Phospho-proteomics, Enzyme-linked Immunosorbent Assay, Transfection