293f Search Results


gfp expression in 293f  (Amaxa)
90
Amaxa gfp expression in 293f
Panel a – Expression and secretion of the laminin β3 from the pcDNA3.1-LAMB3 after transient transfection into H-JEB keratinocytes. Arrows on the left point to positions of molecular weight markers, arrows in the middle point to the β3 subunit. Panels b and c – GFP expression in <t>293F</t> after transient transfection using 293Fectin transfection reagent (b) or Amaxa-based nucleofection (c). Panels d and e – Western blot analysis of the recombinant β3 chain secretion into culture media after transient transfection (d) or nucleofection (e) at various time points. Lane 1 – 48 hr, mock transfected 293F cells. Lanes 2, 3, and 4 - 24, 48, and 72 hr after pcDNA3.1-LAMB3 transfection/nucleofection, respectively. For the analysis of proteins by Western blotting, 5 μg of total protein from cell lysates and 15 μl of culture media were loaded per lane.
Gfp Expression In 293f, supplied by Amaxa, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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suspension expi293f cells  (Fisher Scientific)
90
Fisher Scientific suspension expi293f cells
Panel a – Expression and secretion of the laminin β3 from the pcDNA3.1-LAMB3 after transient transfection into H-JEB keratinocytes. Arrows on the left point to positions of molecular weight markers, arrows in the middle point to the β3 subunit. Panels b and c – GFP expression in <t>293F</t> after transient transfection using 293Fectin transfection reagent (b) or Amaxa-based nucleofection (c). Panels d and e – Western blot analysis of the recombinant β3 chain secretion into culture media after transient transfection (d) or nucleofection (e) at various time points. Lane 1 – 48 hr, mock transfected 293F cells. Lanes 2, 3, and 4 - 24, 48, and 72 hr after pcDNA3.1-LAMB3 transfection/nucleofection, respectively. For the analysis of proteins by Western blotting, 5 μg of total protein from cell lysates and 15 μl of culture media were loaded per lane.
Suspension Expi293f Cells, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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freestyletm 293-f cell line expressing herg(s1-coil)  (Vectalys Inc)
90
Vectalys Inc freestyletm 293-f cell line expressing herg(s1-coil)
Panel a – Expression and secretion of the laminin β3 from the pcDNA3.1-LAMB3 after transient transfection into H-JEB keratinocytes. Arrows on the left point to positions of molecular weight markers, arrows in the middle point to the β3 subunit. Panels b and c – GFP expression in <t>293F</t> after transient transfection using 293Fectin transfection reagent (b) or Amaxa-based nucleofection (c). Panels d and e – Western blot analysis of the recombinant β3 chain secretion into culture media after transient transfection (d) or nucleofection (e) at various time points. Lane 1 – 48 hr, mock transfected 293F cells. Lanes 2, 3, and 4 - 24, 48, and 72 hr after pcDNA3.1-LAMB3 transfection/nucleofection, respectively. For the analysis of proteins by Western blotting, 5 μg of total protein from cell lysates and 15 μl of culture media were loaded per lane.
Freestyletm 293 F Cell Line Expressing Herg(S1 Coil), supplied by Vectalys Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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freestyle™ 293-f cells  (Sino Biological)
90
Sino Biological freestyle™ 293-f cells
Panel a – Expression and secretion of the laminin β3 from the pcDNA3.1-LAMB3 after transient transfection into H-JEB keratinocytes. Arrows on the left point to positions of molecular weight markers, arrows in the middle point to the β3 subunit. Panels b and c – GFP expression in <t>293F</t> after transient transfection using 293Fectin transfection reagent (b) or Amaxa-based nucleofection (c). Panels d and e – Western blot analysis of the recombinant β3 chain secretion into culture media after transient transfection (d) or nucleofection (e) at various time points. Lane 1 – 48 hr, mock transfected 293F cells. Lanes 2, 3, and 4 - 24, 48, and 72 hr after pcDNA3.1-LAMB3 transfection/nucleofection, respectively. For the analysis of proteins by Western blotting, 5 μg of total protein from cell lysates and 15 μl of culture media were loaded per lane.
Freestyle™ 293 F Cells, supplied by Sino Biological, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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expitm 293-f cells  (Polysciences inc)
90
Polysciences inc expitm 293-f cells
Panel a – Expression and secretion of the laminin β3 from the pcDNA3.1-LAMB3 after transient transfection into H-JEB keratinocytes. Arrows on the left point to positions of molecular weight markers, arrows in the middle point to the β3 subunit. Panels b and c – GFP expression in <t>293F</t> after transient transfection using 293Fectin transfection reagent (b) or Amaxa-based nucleofection (c). Panels d and e – Western blot analysis of the recombinant β3 chain secretion into culture media after transient transfection (d) or nucleofection (e) at various time points. Lane 1 – 48 hr, mock transfected 293F cells. Lanes 2, 3, and 4 - 24, 48, and 72 hr after pcDNA3.1-LAMB3 transfection/nucleofection, respectively. For the analysis of proteins by Western blotting, 5 μg of total protein from cell lysates and 15 μl of culture media were loaded per lane.
Expitm 293 F Cells, supplied by Polysciences inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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recombinant human ctla-4 extracellular domain (ecd) protein with a his-tag  (WuXi Biologics)
90
WuXi Biologics recombinant human ctla-4 extracellular domain (ecd) protein with a his-tag
Panel a – Expression and secretion of the laminin β3 from the pcDNA3.1-LAMB3 after transient transfection into H-JEB keratinocytes. Arrows on the left point to positions of molecular weight markers, arrows in the middle point to the β3 subunit. Panels b and c – GFP expression in <t>293F</t> after transient transfection using 293Fectin transfection reagent (b) or Amaxa-based nucleofection (c). Panels d and e – Western blot analysis of the recombinant β3 chain secretion into culture media after transient transfection (d) or nucleofection (e) at various time points. Lane 1 – 48 hr, mock transfected 293F cells. Lanes 2, 3, and 4 - 24, 48, and 72 hr after pcDNA3.1-LAMB3 transfection/nucleofection, respectively. For the analysis of proteins by Western blotting, 5 μg of total protein from cell lysates and 15 μl of culture media were loaded per lane.
Recombinant Human Ctla 4 Extracellular Domain (Ecd) Protein With A His Tag, supplied by WuXi Biologics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hek 293-f cells  (Leidos Biomedical)
90
Leidos Biomedical hek 293-f cells
Panel a – Expression and secretion of the laminin β3 from the pcDNA3.1-LAMB3 after transient transfection into H-JEB keratinocytes. Arrows on the left point to positions of molecular weight markers, arrows in the middle point to the β3 subunit. Panels b and c – GFP expression in <t>293F</t> after transient transfection using 293Fectin transfection reagent (b) or Amaxa-based nucleofection (c). Panels d and e – Western blot analysis of the recombinant β3 chain secretion into culture media after transient transfection (d) or nucleofection (e) at various time points. Lane 1 – 48 hr, mock transfected 293F cells. Lanes 2, 3, and 4 - 24, 48, and 72 hr after pcDNA3.1-LAMB3 transfection/nucleofection, respectively. For the analysis of proteins by Western blotting, 5 μg of total protein from cell lysates and 15 μl of culture media were loaded per lane.
Hek 293 F Cells, supplied by Leidos Biomedical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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293f-expressed recombinant ha proteins from the following influenza strains  (Immune Technology Corp)
90
Immune Technology Corp 293f-expressed recombinant ha proteins from the following influenza strains
Panel a – Expression and secretion of the laminin β3 from the pcDNA3.1-LAMB3 after transient transfection into H-JEB keratinocytes. Arrows on the left point to positions of molecular weight markers, arrows in the middle point to the β3 subunit. Panels b and c – GFP expression in <t>293F</t> after transient transfection using 293Fectin transfection reagent (b) or Amaxa-based nucleofection (c). Panels d and e – Western blot analysis of the recombinant β3 chain secretion into culture media after transient transfection (d) or nucleofection (e) at various time points. Lane 1 – 48 hr, mock transfected 293F cells. Lanes 2, 3, and 4 - 24, 48, and 72 hr after pcDNA3.1-LAMB3 transfection/nucleofection, respectively. For the analysis of proteins by Western blotting, 5 μg of total protein from cell lysates and 15 μl of culture media were loaded per lane.
293f Expressed Recombinant Ha Proteins From The Following Influenza Strains, supplied by Immune Technology Corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dii labeled 293f exosomes  (Jackson Laboratory)
90
Jackson Laboratory dii labeled 293f exosomes
(A) MSC <t>exosomes</t> concentration and size distribution using nanoparticle tracking analysis (NTA). (B) Transmission electron microscopy images of exosomes. Scale bar, 100 nm. (C) Western blots showing the expression of exosomes and cellular markers, from MSC and MSC exosome lysates. (D) MYC mRNA expression in U87 cells transfected with Ctrl-siRNA#1 and Myc-siRNA#1 for 24 hours. One-way ANOVA with Dunnett’s multiple comparisons test was performed based on ΔCT values. (E) MYC mRNA expression in U87 cells transfected with Ctrl-siRNA#2 and Myc-siRNA#2 for 24 hours. One-way ANOVA with Dunnett’s multiple comparisons test was performed based on ΔCT values. (F) Cell viability of U87 cells, transfected with Ctrl-siRNA#2 and Myc-siRNA#1 Ctrl-siRNA#1 and Myc-siRNA#2 for 48 hours. One-way ANOVA with Dunnett’s multiple comparisons test was performed based on raw 560 nm absorbance values. (G) In vivo bioluminescence and ex vivo fluorescence imaging of non-tumor-bearing mice and luciferase-expressing GL261 intracranial tumor-bearing mice, 6 hours after i.v. injection of DiR-labeled MSC exosomes. Exact p-values are reported.
Dii Labeled 293f Exosomes, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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293f-expressed recombinant ha proteins  (eEnzyme Inc)
90
eEnzyme Inc 293f-expressed recombinant ha proteins
(A) MSC <t>exosomes</t> concentration and size distribution using nanoparticle tracking analysis (NTA). (B) Transmission electron microscopy images of exosomes. Scale bar, 100 nm. (C) Western blots showing the expression of exosomes and cellular markers, from MSC and MSC exosome lysates. (D) MYC mRNA expression in U87 cells transfected with Ctrl-siRNA#1 and Myc-siRNA#1 for 24 hours. One-way ANOVA with Dunnett’s multiple comparisons test was performed based on ΔCT values. (E) MYC mRNA expression in U87 cells transfected with Ctrl-siRNA#2 and Myc-siRNA#2 for 24 hours. One-way ANOVA with Dunnett’s multiple comparisons test was performed based on ΔCT values. (F) Cell viability of U87 cells, transfected with Ctrl-siRNA#2 and Myc-siRNA#1 Ctrl-siRNA#1 and Myc-siRNA#2 for 48 hours. One-way ANOVA with Dunnett’s multiple comparisons test was performed based on raw 560 nm absorbance values. (G) In vivo bioluminescence and ex vivo fluorescence imaging of non-tumor-bearing mice and luciferase-expressing GL261 intracranial tumor-bearing mice, 6 hours after i.v. injection of DiR-labeled MSC exosomes. Exact p-values are reported.
293f Expressed Recombinant Ha Proteins, supplied by eEnzyme Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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293f cells  (Alphamab Co Ltd)
90
Alphamab Co Ltd 293f cells
(A) MSC <t>exosomes</t> concentration and size distribution using nanoparticle tracking analysis (NTA). (B) Transmission electron microscopy images of exosomes. Scale bar, 100 nm. (C) Western blots showing the expression of exosomes and cellular markers, from MSC and MSC exosome lysates. (D) MYC mRNA expression in U87 cells transfected with Ctrl-siRNA#1 and Myc-siRNA#1 for 24 hours. One-way ANOVA with Dunnett’s multiple comparisons test was performed based on ΔCT values. (E) MYC mRNA expression in U87 cells transfected with Ctrl-siRNA#2 and Myc-siRNA#2 for 24 hours. One-way ANOVA with Dunnett’s multiple comparisons test was performed based on ΔCT values. (F) Cell viability of U87 cells, transfected with Ctrl-siRNA#2 and Myc-siRNA#1 Ctrl-siRNA#1 and Myc-siRNA#2 for 48 hours. One-way ANOVA with Dunnett’s multiple comparisons test was performed based on raw 560 nm absorbance values. (G) In vivo bioluminescence and ex vivo fluorescence imaging of non-tumor-bearing mice and luciferase-expressing GL261 intracranial tumor-bearing mice, 6 hours after i.v. injection of DiR-labeled MSC exosomes. Exact p-values are reported.
293f Cells, supplied by Alphamab Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human embryonic kidney 293-f suspension cells  (Novartis)
90
Novartis human embryonic kidney 293-f suspension cells
(A) MSC <t>exosomes</t> concentration and size distribution using nanoparticle tracking analysis (NTA). (B) Transmission electron microscopy images of exosomes. Scale bar, 100 nm. (C) Western blots showing the expression of exosomes and cellular markers, from MSC and MSC exosome lysates. (D) MYC mRNA expression in U87 cells transfected with Ctrl-siRNA#1 and Myc-siRNA#1 for 24 hours. One-way ANOVA with Dunnett’s multiple comparisons test was performed based on ΔCT values. (E) MYC mRNA expression in U87 cells transfected with Ctrl-siRNA#2 and Myc-siRNA#2 for 24 hours. One-way ANOVA with Dunnett’s multiple comparisons test was performed based on ΔCT values. (F) Cell viability of U87 cells, transfected with Ctrl-siRNA#2 and Myc-siRNA#1 Ctrl-siRNA#1 and Myc-siRNA#2 for 48 hours. One-way ANOVA with Dunnett’s multiple comparisons test was performed based on raw 560 nm absorbance values. (G) In vivo bioluminescence and ex vivo fluorescence imaging of non-tumor-bearing mice and luciferase-expressing GL261 intracranial tumor-bearing mice, 6 hours after i.v. injection of DiR-labeled MSC exosomes. Exact p-values are reported.
Human Embryonic Kidney 293 F Suspension Cells, supplied by Novartis, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Panel a – Expression and secretion of the laminin β3 from the pcDNA3.1-LAMB3 after transient transfection into H-JEB keratinocytes. Arrows on the left point to positions of molecular weight markers, arrows in the middle point to the β3 subunit. Panels b and c – GFP expression in 293F after transient transfection using 293Fectin transfection reagent (b) or Amaxa-based nucleofection (c). Panels d and e – Western blot analysis of the recombinant β3 chain secretion into culture media after transient transfection (d) or nucleofection (e) at various time points. Lane 1 – 48 hr, mock transfected 293F cells. Lanes 2, 3, and 4 - 24, 48, and 72 hr after pcDNA3.1-LAMB3 transfection/nucleofection, respectively. For the analysis of proteins by Western blotting, 5 μg of total protein from cell lysates and 15 μl of culture media were loaded per lane.

Journal: The Journal of Investigative Dermatology

Article Title: PROTEIN THERAPEUTICS FOR JUNCTIONAL EPIDERMOLYSIS BULLOSA: INCORPORATION OF RECOMBINANT ?3 CHAIN INTO LAMININ 332 IN ?3-/- KERATINOCYTES IN VITRO

doi: 10.1038/sj.jid.5701197

Figure Lengend Snippet: Panel a – Expression and secretion of the laminin β3 from the pcDNA3.1-LAMB3 after transient transfection into H-JEB keratinocytes. Arrows on the left point to positions of molecular weight markers, arrows in the middle point to the β3 subunit. Panels b and c – GFP expression in 293F after transient transfection using 293Fectin transfection reagent (b) or Amaxa-based nucleofection (c). Panels d and e – Western blot analysis of the recombinant β3 chain secretion into culture media after transient transfection (d) or nucleofection (e) at various time points. Lane 1 – 48 hr, mock transfected 293F cells. Lanes 2, 3, and 4 - 24, 48, and 72 hr after pcDNA3.1-LAMB3 transfection/nucleofection, respectively. For the analysis of proteins by Western blotting, 5 μg of total protein from cell lysates and 15 μl of culture media were loaded per lane.

Article Snippet: Panels b and c – GFP expression in 293F after transient transfection using 293Fectin transfection reagent (b) or Amaxa-based nucleofection (c).

Techniques: Expressing, Transfection, Molecular Weight, Western Blot, Recombinant

Panel a, lane 1 – concentrated culture media collected from β3 producing 293F cells; lane 2 - fraction containing laminin β3 after gel filtration. Samples of culture media and purified protein were prepared under denaturing condition, separated on 4-12% gradient PAGE, and stained with GelCode Blue reagent. Arrows on the left point to positions of molecular weight markers, arrow on the right points to the β3 subunit of laminin 332. Panels b, c, d, e – delivery of the recombinant β-galactosidase into normal (b, d) and H-JEB (c, e) keratinocytes. Panels b and c – photographs of protein transfected wells stained for the β-galactosidase activity; Panels d and e – magnified fields of panels b and c, respectively.

Journal: The Journal of Investigative Dermatology

Article Title: PROTEIN THERAPEUTICS FOR JUNCTIONAL EPIDERMOLYSIS BULLOSA: INCORPORATION OF RECOMBINANT ?3 CHAIN INTO LAMININ 332 IN ?3-/- KERATINOCYTES IN VITRO

doi: 10.1038/sj.jid.5701197

Figure Lengend Snippet: Panel a, lane 1 – concentrated culture media collected from β3 producing 293F cells; lane 2 - fraction containing laminin β3 after gel filtration. Samples of culture media and purified protein were prepared under denaturing condition, separated on 4-12% gradient PAGE, and stained with GelCode Blue reagent. Arrows on the left point to positions of molecular weight markers, arrow on the right points to the β3 subunit of laminin 332. Panels b, c, d, e – delivery of the recombinant β-galactosidase into normal (b, d) and H-JEB (c, e) keratinocytes. Panels b and c – photographs of protein transfected wells stained for the β-galactosidase activity; Panels d and e – magnified fields of panels b and c, respectively.

Article Snippet: Panels b and c – GFP expression in 293F after transient transfection using 293Fectin transfection reagent (b) or Amaxa-based nucleofection (c).

Techniques: Filtration, Purification, Staining, Molecular Weight, Recombinant, Transfection, Activity Assay

(A) MSC exosomes concentration and size distribution using nanoparticle tracking analysis (NTA). (B) Transmission electron microscopy images of exosomes. Scale bar, 100 nm. (C) Western blots showing the expression of exosomes and cellular markers, from MSC and MSC exosome lysates. (D) MYC mRNA expression in U87 cells transfected with Ctrl-siRNA#1 and Myc-siRNA#1 for 24 hours. One-way ANOVA with Dunnett’s multiple comparisons test was performed based on ΔCT values. (E) MYC mRNA expression in U87 cells transfected with Ctrl-siRNA#2 and Myc-siRNA#2 for 24 hours. One-way ANOVA with Dunnett’s multiple comparisons test was performed based on ΔCT values. (F) Cell viability of U87 cells, transfected with Ctrl-siRNA#2 and Myc-siRNA#1 Ctrl-siRNA#1 and Myc-siRNA#2 for 48 hours. One-way ANOVA with Dunnett’s multiple comparisons test was performed based on raw 560 nm absorbance values. (G) In vivo bioluminescence and ex vivo fluorescence imaging of non-tumor-bearing mice and luciferase-expressing GL261 intracranial tumor-bearing mice, 6 hours after i.v. injection of DiR-labeled MSC exosomes. Exact p-values are reported.

Journal: Extracellular vesicle

Article Title: Engineered exosomes targeting MYC reverse the proneural-mesenchymal transition and extend survival of glioblastoma

doi: 10.1016/j.vesic.2022.100014

Figure Lengend Snippet: (A) MSC exosomes concentration and size distribution using nanoparticle tracking analysis (NTA). (B) Transmission electron microscopy images of exosomes. Scale bar, 100 nm. (C) Western blots showing the expression of exosomes and cellular markers, from MSC and MSC exosome lysates. (D) MYC mRNA expression in U87 cells transfected with Ctrl-siRNA#1 and Myc-siRNA#1 for 24 hours. One-way ANOVA with Dunnett’s multiple comparisons test was performed based on ΔCT values. (E) MYC mRNA expression in U87 cells transfected with Ctrl-siRNA#2 and Myc-siRNA#2 for 24 hours. One-way ANOVA with Dunnett’s multiple comparisons test was performed based on ΔCT values. (F) Cell viability of U87 cells, transfected with Ctrl-siRNA#2 and Myc-siRNA#1 Ctrl-siRNA#1 and Myc-siRNA#2 for 48 hours. One-way ANOVA with Dunnett’s multiple comparisons test was performed based on raw 560 nm absorbance values. (G) In vivo bioluminescence and ex vivo fluorescence imaging of non-tumor-bearing mice and luciferase-expressing GL261 intracranial tumor-bearing mice, 6 hours after i.v. injection of DiR-labeled MSC exosomes. Exact p-values are reported.

Article Snippet: DiI labeled 293F exosomes were injected (1x10 10 /100 μl/mouse) via retro-orbital sinus in non-tumor-bearing C57BL/6J mice (Jackson Laboratory).

Techniques: Concentration Assay, Transmission Assay, Electron Microscopy, Western Blot, Expressing, Transfection, In Vivo, Ex Vivo, Fluorescence, Imaging, Luciferase, Injection, Labeling

(A) Experimental design of treatment with intravenous (i.v.) injected exosomes. (B) Bioluminescence measurement per mouse in Exo-Ctrl#2 and iExo-Myc#2 treated groups over the dosing period. iExo-Ctrl#2, N=5; iExo-Myc#2, N=8. (C-D) Histological analyses of whole brain (C) and tumor regions (D) H&E stained tissues. (C) Scale bar, 1000 μm. (D) Scale bar, 50 μm. (E) Kaplan-Meier survival curves of iExo-Ctrl#2 and iExo-Myc#2 treated groups, compared using log-rank (Mantel-Cox) test. iExo-Ctrl#2, N=5; iExo-Myc#2, N=8. (F) Immunohistochemical detection of Myc, Ki67 and CD31 in U87 brain tumor sections of iExo-Ctrl#2 and iExo-Myc#2 treated samples. Scale bar, 200 μm. (F) Quantification of Myc, Ki67 and CD31 positive cells. Mann Whitney test was performed for Myc. Unpaired t test was performed for Ki67 and CD31. Myc: iExo-Ctrl#2, N=4; iExo-Myc#2, N=8. Ki67: iExo-Ctrl#2, N=4; iExo-Myc#2, N=4. CD31: iExo-Ctrl#2, N=4; iExo-Myc#2, N=4. Exact p-values are reported.

Journal: Extracellular vesicle

Article Title: Engineered exosomes targeting MYC reverse the proneural-mesenchymal transition and extend survival of glioblastoma

doi: 10.1016/j.vesic.2022.100014

Figure Lengend Snippet: (A) Experimental design of treatment with intravenous (i.v.) injected exosomes. (B) Bioluminescence measurement per mouse in Exo-Ctrl#2 and iExo-Myc#2 treated groups over the dosing period. iExo-Ctrl#2, N=5; iExo-Myc#2, N=8. (C-D) Histological analyses of whole brain (C) and tumor regions (D) H&E stained tissues. (C) Scale bar, 1000 μm. (D) Scale bar, 50 μm. (E) Kaplan-Meier survival curves of iExo-Ctrl#2 and iExo-Myc#2 treated groups, compared using log-rank (Mantel-Cox) test. iExo-Ctrl#2, N=5; iExo-Myc#2, N=8. (F) Immunohistochemical detection of Myc, Ki67 and CD31 in U87 brain tumor sections of iExo-Ctrl#2 and iExo-Myc#2 treated samples. Scale bar, 200 μm. (F) Quantification of Myc, Ki67 and CD31 positive cells. Mann Whitney test was performed for Myc. Unpaired t test was performed for Ki67 and CD31. Myc: iExo-Ctrl#2, N=4; iExo-Myc#2, N=8. Ki67: iExo-Ctrl#2, N=4; iExo-Myc#2, N=4. CD31: iExo-Ctrl#2, N=4; iExo-Myc#2, N=4. Exact p-values are reported.

Article Snippet: DiI labeled 293F exosomes were injected (1x10 10 /100 μl/mouse) via retro-orbital sinus in non-tumor-bearing C57BL/6J mice (Jackson Laboratory).

Techniques: Injection, Staining, Immunohistochemical staining, MANN-WHITNEY

(A) Experimental design of treatment with i.v. injected exosomes (B) Bioluminescence measurement per mouse over the dosing period with mice divided into two groups: small tumors (BLI at day 23 < 1x106) and large tumors (BLI at day 23 < 1x106). Plasmalyte + Myc siRNA#1, N=9; iExo-Ctrl#1, N=10; iExo-Myc#1, N=10. (C-D) Histological analyses of whole brain (C) and tumor regions (D) H&E stained tissues. (C) Scale bar, 500 μm. (D) Scale bar, 50 μm. (E) Kaplan-Meier survival curve of animals treated via i.v. injection. Log-rank (Mantel-Cox) test was performed. Plasmalyte + Myc siRNA#1, N=9; iExo-Ctrl#1, N=10; iExo-Myc#1, N=10. (F) Immunohistochemical detection of Myc, Ki67 and CD31 in U87 brain tumor sections treated with iExosomes administered i.v. Scale bar, 50 μm. (G) Quantification of Myc, Ki67 and CD31 positive cells. IRS, immunoreactive score. Kruskall-Wallis with Dunn’s multiple comparisons test was performed for Myc and CD31. One-way ANOVA with Dunnett’s multiple comparisons test was performed for Ki67. Myc: Plasmalyte + Myc siRNA#1, N=6; iExo-Ctrl#1, N=8; iExo-Myc#1, N=8. Ki67: Plasmalyte + Myc siRNA#1, N=6; iExo-Ctrl#1, N=6; and iExo-Myc#1, N=6. CD31: Plasmalyte + Myc siRNA#1, N=6; iExo-Ctrl#1, N=6; iExo-Myc#1, N=6. (H) Experimental design of treatment with intranasal (i.n.) administered exosomes. (I) Kaplan-Meier survival curve of mice treated i.n. Log-rank (Mantel-Cox) test was performed. Untreated control group, N=5; iExo-Ctrl#1, N=7; iExo-Myc#1, N=7. Exact p-values are reported.

Journal: Extracellular vesicle

Article Title: Engineered exosomes targeting MYC reverse the proneural-mesenchymal transition and extend survival of glioblastoma

doi: 10.1016/j.vesic.2022.100014

Figure Lengend Snippet: (A) Experimental design of treatment with i.v. injected exosomes (B) Bioluminescence measurement per mouse over the dosing period with mice divided into two groups: small tumors (BLI at day 23 < 1x106) and large tumors (BLI at day 23 < 1x106). Plasmalyte + Myc siRNA#1, N=9; iExo-Ctrl#1, N=10; iExo-Myc#1, N=10. (C-D) Histological analyses of whole brain (C) and tumor regions (D) H&E stained tissues. (C) Scale bar, 500 μm. (D) Scale bar, 50 μm. (E) Kaplan-Meier survival curve of animals treated via i.v. injection. Log-rank (Mantel-Cox) test was performed. Plasmalyte + Myc siRNA#1, N=9; iExo-Ctrl#1, N=10; iExo-Myc#1, N=10. (F) Immunohistochemical detection of Myc, Ki67 and CD31 in U87 brain tumor sections treated with iExosomes administered i.v. Scale bar, 50 μm. (G) Quantification of Myc, Ki67 and CD31 positive cells. IRS, immunoreactive score. Kruskall-Wallis with Dunn’s multiple comparisons test was performed for Myc and CD31. One-way ANOVA with Dunnett’s multiple comparisons test was performed for Ki67. Myc: Plasmalyte + Myc siRNA#1, N=6; iExo-Ctrl#1, N=8; iExo-Myc#1, N=8. Ki67: Plasmalyte + Myc siRNA#1, N=6; iExo-Ctrl#1, N=6; and iExo-Myc#1, N=6. CD31: Plasmalyte + Myc siRNA#1, N=6; iExo-Ctrl#1, N=6; iExo-Myc#1, N=6. (H) Experimental design of treatment with intranasal (i.n.) administered exosomes. (I) Kaplan-Meier survival curve of mice treated i.n. Log-rank (Mantel-Cox) test was performed. Untreated control group, N=5; iExo-Ctrl#1, N=7; iExo-Myc#1, N=7. Exact p-values are reported.

Article Snippet: DiI labeled 293F exosomes were injected (1x10 10 /100 μl/mouse) via retro-orbital sinus in non-tumor-bearing C57BL/6J mice (Jackson Laboratory).

Techniques: Injection, Staining, Immunohistochemical staining, Control