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Image Search Results
Journal: Immunology
Article Title: Frequency and phenotype of natural killer cells and natural killer cell subsets in bovine lymphoid compartments and blood
doi: 10.1111/imm.12708
Figure Lengend Snippet: CD2 − natural killer (NK) cells are the predominant subset of NK cells present within skin‐draining afferent lymph (AL). Lymphocytes derived from peripheral blood (PB), AL and the lymph nodes (LNs) of seven calves were labelled with monoclonal antibodies to NKp46, CD3 and CD2 and analysed by flow cytometry. FACS plots from one representative animal illustrate the expression of NKp46 and CD3 by PB‐, AL‐ and LN‐derived lymphocytes (a). FACS plots from one representative animal illustrate the expression of NKp46 and CD2 by PB‐, AL‐ and LN‐derived lymphocytes (b). Gates were set based on Fluorescence Minus One controls. Pooled data from seven calves indicate the average percentage of NKp46 + CD3 − NK cells ± SD present in PB (circles), AL (squares) and LNs (triangles) (c). Pooled data from seven calves illustrate the average percentage of CD2 + (lighter bars) and CD2 − (darker bars) NK cells ± SD within the total gated NKp46 + NK cell population from PB, AL or the LNs (d). Data were normally distributed ( P > 0·05). Significance between PB and AL was assessed using a paired t ‐test and significance between PB and LN was assessed using a two‐sample t ‐test. P < 0·001***. [Colour figure can be viewed at wileyonlinelibrary.com ]
Article Snippet: NK cells were identified by labelling cells with mouse
Techniques: Derivative Assay, Flow Cytometry, Expressing, Fluorescence
Journal: Immunology
Article Title: Frequency and phenotype of natural killer cells and natural killer cell subsets in bovine lymphoid compartments and blood
doi: 10.1111/imm.12708
Figure Lengend Snippet: Natural killer (NK) cells are present in bovine efferent lymph and CD2 − NK cells are the principal subset present. Lymphocytes derived from peripheral blood (PB), afferent lymph (AL), lymph nodes (LNs) and efferent lymph (EL) were labelled with monoclonal antibodies for NKp46, CD3 and CD2 and analysed by flow cytometry. Pooled data from seven calves for PB, AL and LNs and five calves for EL, show the average percentage of NKp46 + CD3 − NK cells ± SD present within PB (circles), AL (squares), LNs (triangles) and EL (diamonds) (a). Pooled data from seven (PB, AL and LNs) and five (EL) calves illustrate the average percentage of NKp46 + CD2 + (lighter bars) and NKp46 + CD2 − (darker bars) NK cells ± SD within the total gated NKp46 + NK cell population from PB, AL, LNs and five calves for EL (b). Data were normally distributed ( P > 0·05) and significance between PB and EL was assessed using a two‐sample t ‐test. P < 0·01**, P < 0·001***.
Article Snippet: NK cells were identified by labelling cells with mouse
Techniques: Derivative Assay, Flow Cytometry