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Image Search Results
Journal: Frontiers in Immunology
Article Title: Degradation of IL-4Ralpha by Immunoproteasome: implication in airway type 2 inflammation and hyperresponsiveness
doi: 10.3389/fimmu.2025.1501898
Figure Lengend Snippet: Inhibition of immunoproteasome (IP) increases IL-4Rα and eotaxin-3 in IL-13-stimulated human airway epithelial cells. Tracheobronchial epithelial cells from n=6 healthy donors were pre-treated with immunoproteasome LMP7 inhibitor ONX-0914, then given anti-IL4Rα antibody followed by IL-13 treatment. IP inhibition in IL-13 treated cells significantly upregulated IL-4Rα protein levels five hours after ONX-0914 treatment (A) , as well as significantly increased eotaxin-3 levels after 72 hours (B) . Anti-IL4Rα antibody significantly inhibited IL-13-induced eotaxin-3.
Article Snippet: Equal amounts of proteins were loaded and separated by SDS-PAGE, transferred onto PVDF membranes, blocked with blocking buffer, and incubated with the following primary antibodies overnight at 4°C:
Techniques: Inhibition